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Article Open Access

Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling

  • Authors:
    • Ge Zhang
    • Tao Fang
    • Meijia Chang
    • Jing Li
    • Qunying Hong
    • Chunxue Bai
    • Jian Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pulmonary Medicine, Shanghai Respiratory Research Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Oncology, Shengli Oilfield Central Hospital, Dongying, Shandong 257034, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1937-1946
    |
    Published online on: August 2, 2018
       https://doi.org/10.3892/or.2018.6625
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Abstract

Gefitinib, an epidermal growth factor receptor (EGFR)‑specific drug, is effective for ~1 year, after which resistance is inevitable. Calpain 2 (CAPN2) is known to serve a role in the drug response and resistance in certain cancer therapies. However, the full function of CAPN2, particularly in non‑small cell lung cancer, has not yet been elucidated. In the present study, CAPN2 expression in gefitinib‑resistant lung adenocarcinoma cells was investigated. CAPN2 function in these cells was further evaluated using gene knockdown both in vitro and in vivo. The results demonstrated that CAPN2 was strongly associated with gefitinib‑resistance, and CAPN2 mRNA and protein expression levels were significantly increased in gefitinib‑resistant cell lines. Furthermore, CAPN2 knockdown inhibited gefitinib‑resistant cell proliferation in vitro and in vivo. CAPN2 conferred gefitinib‑resistance by inhibiting cell apoptosis and arresting the cell cycle. CAPN2 knockdown also induced caspase activation and mitochondrial dysfunction, and its function in gefitinib resistance appeared to be largely mediated by EGFR/protein kinase B/survivin signaling pathway activation. These results suggest that CAPN2 is responsible for EGFR‑tyrosine kinase inhibitor resistance, and CAPN2 inhibition may be used to provide therapeutic benefits in the treatment of gefitinib resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang G, Fang T, Chang M, Li J, Hong Q, Bai C and Zhou J: Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncol Rep 40: 1937-1946, 2018.
APA
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., & Zhou, J. (2018). Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncology Reports, 40, 1937-1946. https://doi.org/10.3892/or.2018.6625
MLA
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., Zhou, J."Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling". Oncology Reports 40.4 (2018): 1937-1946.
Chicago
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., Zhou, J."Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling". Oncology Reports 40, no. 4 (2018): 1937-1946. https://doi.org/10.3892/or.2018.6625
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang G, Fang T, Chang M, Li J, Hong Q, Bai C and Zhou J: Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncol Rep 40: 1937-1946, 2018.
APA
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., & Zhou, J. (2018). Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling. Oncology Reports, 40, 1937-1946. https://doi.org/10.3892/or.2018.6625
MLA
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., Zhou, J."Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling". Oncology Reports 40.4 (2018): 1937-1946.
Chicago
Zhang, G., Fang, T., Chang, M., Li, J., Hong, Q., Bai, C., Zhou, J."Calpain 2 knockdown promotes cell apoptosis and restores gefitinib sensitivity through epidermal growth factor receptor/protein kinase B/survivin signaling". Oncology Reports 40, no. 4 (2018): 1937-1946. https://doi.org/10.3892/or.2018.6625
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