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Article

Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis

  • Authors:
    • Jinhua Wang
    • Xiaohui Yang
    • Rong Li
    • Liang Wang
    • Yinmin Gu
    • Yuhui Zhao
    • Kuo Hsiang Huang
    • Tianyou Cheng
    • Yi Yuan
    • Shan Gao
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China, CAS Key Laboratory of Bio-medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, P.R. China, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China, Shanxi Academy of Advanced Research and Innovation, Taiyuan, Shanxi 030032, P.R. China, Medical College, Guizhou University, Guiyang, Guizhou 550025, P.R. China
  • Pages: 2814-2825
    |
    Published online on: August 20, 2018
       https://doi.org/10.3892/or.2018.6661
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Abstract

Long non-coding RNAs (lncRNAs) play critical roles in tumorigenesis and cancer progression. The c-Myc upregulated lncRNA MYU (VPS9D1 antisense RNA1, annotated as VPS9D1-AS1) has been reported in several common types of human cancers, which has revealed that lncRNA MYU could function as either an oncogene or a tumor-suppressor gene in different cancer types. However, the function of lncRNA MYU in prostate cancer remains unknown. In the present study, we demonstrated that lncRNA MYU is significantly upregulated in prostate cancer tissues. MYU knockdown impaired prostate cancer cell growth and migration as shown from cell viability, colony formation, Transwell and wound healing assays. In contrast, MYU overexpression displayed opposite effects. No correlation was noted between MYU and its cognate VPS9D1 expression level. Moreover, lncRNA MYU did not regulate the expression of VPS9D1 either at the mRNA or protein level as detected using qRT-PCR and western blotting assays. Furthermore, lncRNA MYU was able to be transported into the extracellular milieu by means of exosomes, and then promoted adjacent cell proliferation and migration. Mechanistically, lncRNA MYU upregulated c-Myc by competitively binding miR-184 and then induced the proliferation of prostate cancer. Thus, this study demonstrated that lncRNA MYU functions as an oncogene in prostate cancer at least in part through the miR-184/c-Myc axis, and may serve as a potential diagnostic biomarker and therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Yang X, Li R, Wang L, Gu Y, Zhao Y, Huang KH, Cheng T, Yuan Y, Gao S, Gao S, et al: Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis. Oncol Rep 40: 2814-2825, 2018.
APA
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y. ... Gao, S. (2018). Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis. Oncology Reports, 40, 2814-2825. https://doi.org/10.3892/or.2018.6661
MLA
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y., Huang, K. H., Cheng, T., Yuan, Y., Gao, S."Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis". Oncology Reports 40.5 (2018): 2814-2825.
Chicago
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y., Huang, K. H., Cheng, T., Yuan, Y., Gao, S."Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis". Oncology Reports 40, no. 5 (2018): 2814-2825. https://doi.org/10.3892/or.2018.6661
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Yang X, Li R, Wang L, Gu Y, Zhao Y, Huang KH, Cheng T, Yuan Y, Gao S, Gao S, et al: Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis. Oncol Rep 40: 2814-2825, 2018.
APA
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y. ... Gao, S. (2018). Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis. Oncology Reports, 40, 2814-2825. https://doi.org/10.3892/or.2018.6661
MLA
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y., Huang, K. H., Cheng, T., Yuan, Y., Gao, S."Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis". Oncology Reports 40.5 (2018): 2814-2825.
Chicago
Wang, J., Yang, X., Li, R., Wang, L., Gu, Y., Zhao, Y., Huang, K. H., Cheng, T., Yuan, Y., Gao, S."Long non-coding RNA MYU promotes prostate cancer proliferation by mediating the miR-184/c-Myc axis". Oncology Reports 40, no. 5 (2018): 2814-2825. https://doi.org/10.3892/or.2018.6661
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