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Article Open Access

Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing

  • Authors:
    • Qiang Huang
    • Jiechao Yang
    • Juan Zheng
    • Chiyao Hsueh
    • Yang Guo
    • Liang Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2584-2594
    |
    Published online on: August 24, 2018
       https://doi.org/10.3892/or.2018.6672
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Abstract

Exosomes are nanometer‑scale extracellular vesicles derived from almost all types of cells and key signaling mediators between cancer cells and their microenvironment. Certain microRNAs (miRNAs) are selected for exosome packing and exclusion from parental cells, while other miRNAs are selectively retained by cells, suggesting a biological role for these miRNAs in tumor malignant progression. In the present study, we isolated and characterized the exosomes derived from the laryngeal squamous cell carcinoma (LSCC) cell line AMC‑HN‑8 for the first time, and identified a subset of miRNAs enriched in the exosomes compared with parental cells, such as miR‑1246, miR‑1290, miR‑335‑5p, miR‑127‑3p and miR‑122‑5p through small RNA sequencing combined with reverse transcription‑quantitative PCR (RT‑qPCR) analysis. Potential target prediction, Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed possible functions associated with these selective exosomal miRNAs. In conclusion, the present study demonstrated that the LSCC cell line AMC‑HN‑8 can release exosomes and cells can selectively pack certain miRNAs into exosomes.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Q, Yang J, Zheng J, Hsueh C, Guo Y and Zhou L: Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing. Oncol Rep 40: 2584-2594, 2018.
APA
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., & Zhou, L. (2018). Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing. Oncology Reports, 40, 2584-2594. https://doi.org/10.3892/or.2018.6672
MLA
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., Zhou, L."Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing". Oncology Reports 40.5 (2018): 2584-2594.
Chicago
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., Zhou, L."Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing". Oncology Reports 40, no. 5 (2018): 2584-2594. https://doi.org/10.3892/or.2018.6672
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Q, Yang J, Zheng J, Hsueh C, Guo Y and Zhou L: Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing. Oncol Rep 40: 2584-2594, 2018.
APA
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., & Zhou, L. (2018). Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing. Oncology Reports, 40, 2584-2594. https://doi.org/10.3892/or.2018.6672
MLA
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., Zhou, L."Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing". Oncology Reports 40.5 (2018): 2584-2594.
Chicago
Huang, Q., Yang, J., Zheng, J., Hsueh, C., Guo, Y., Zhou, L."Characterization of selective exosomal microRNA expression profile derived from laryngeal squamous cell carcinoma detected by next generation sequencing". Oncology Reports 40, no. 5 (2018): 2584-2594. https://doi.org/10.3892/or.2018.6672
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