Identification of a novel biomarker in tangeretin‑induced cell death in AGS human gastric cancer cells

Yumnam,Silvia; Raha,Suchismita; Kim,Seong   Min; Venkatarame Gowda Saralamma,Venu; Lee,Ho Jeong; Ha,Sang Eun; Heo,Jeong Doo; Lee,Sang Joon; Kim,Eun Hee; Lee,Won Sup; Kim,Jin A.; Kim,Gon Sup

doi:10.3892/or.2018.6730

Identification of a novel biomarker in tangeretin‑induced cell death in AGS human gastric cancer cells

Authors:
- Silvia Yumnam
- Suchismita Raha
- Seong Min Kim
- Venu Venkatarame Gowda Saralamma
- Ho Jeong Lee
- Sang Eun Ha
- Jeong Doo Heo
- Sang Joon Lee
- Eun Hee Kim
- Won Sup Lee
- Jin A. Kim
- Gon Sup Kim
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Affiliations: Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongsang 52828, Republic of Korea, Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongsang 52828, Republic of Korea, Gyeongnam Department of Environment Toxicology and Chemistry, Toxicology Screening Research Center, Korea Institute of Toxicology, Jinju, Gyeongsang 52828, Republic of Korea, Gyeongnam Department of Environment Toxicology and Chemistry, Toxicology Screening Research Center, Korea Institute of Toxicology, Jinju, Gyeongsang 52828, Republic of Korea, Department of Nursing Science, International University of Korea, Jinju, Gyeongsang 52833, Republic of Korea, Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University, School of Medicine, Jinju, Gyeongsang 52727, Republic of Korea, Department of Physical Therapy, International University of Korea, Jinju, Gyeongsang 52833, Republic of Korea
Published online on: September 24, 2018 https://doi.org/10.3892/or.2018.6730
Pages: 3249-3260
Copyright: © Yumnam et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proteomic analysis serves as an important biological tool for identifying biological events. Novel biomarkers of a specific disease such as cancer may be identified using these promising techniques. The aim of the present study was to investigate the effect of tangeretin and to identify potential biomarkers in AGS gastric cancer cells using a proteomics approach. The results of the present study revealed that tangeretin inhibited AGS cell viability dose‑dependently with a half‑maximal inhibitory concentration of 100 µM. Two‑dimensional gel electrophoresis was performed to determine the potential biomarker between control and tangeretin (100 µM)‑treated AGS cells. A total of 16 proteins was identified from 36 significant protein spots using matrix‑assisted laser‑desorption/ionization time‑of‑flight‑mass spectrometry using peptide fingerprinting. The bioinformatics tools Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to identify the functional properties and association of the proteins obtained. Using western blot analysis, the regulatory pattern of four selected proteins, protein kinase Cε, mitogen‑activated protein kinase 4, phosphoinositide 4‑kinase and poly(ADP‑ribose) polymerase 14, were successfully verified in replicate sample sets. These selected proteins are primarily involved in apoptosis signaling, angiogenesis, cell cycle regulation, receptor kinase binding, intracellular cytoplasmic and nuclear alterations. Therefore, aim of the present study was to identify potential diagnostic biomarkers from the functional categories of altered protein expression in tangeretin‑inhibited AGS gastric cancer cell viability.

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