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Article

JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells

  • Authors:
    • Weiguo Liao
    • Jie Liu
    • Bin Liu
    • Xiaojie Huang
    • Yongxin Yin
    • De Cai
    • Mingyi Li
    • Runzhi Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China, Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China, Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
  • Pages: 3812-3820
    |
    Published online on: September 26, 2018
       https://doi.org/10.3892/or.2018.6737
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Abstract

JIB‑04 is a structurally unique small molecule, known to exhibit anticancer activity and to inhibit the growth of human lung cancer and prostate cancer cell lines. However, the anticancer effect of JIB‑04 against human hepatic carcinoma, and its underlying mechanisms, are still unclear. In the present study, MHCC97H and HepG2 cells were employed to investigate the anticancer effects of JIB‑04 on cell viability and apoptosis. Annexin V/PI staining, a CCK‑8 assay and western blot analysis demonstrated that JIB‑04 induced apoptosis in MHCC97H and HepG2 cells, which was evidenced by the expression of proapoptotic and apoptotic proteins including p53, Bak, Bax, caspase‑3 and caspase‑9. Subsequently, the expression trends of Bcl‑2 and p53 were reversed after co‑treatment with pifithrin‑α (PFT‑α, a p53 inhibitor). The results revealed that JIB‑04 suppressed the cell viability of MHCC97H and HepG2 cells in a concentration‑dependent manner. Meanwhile, it was also demonstrated that JIB‑04 effectively triggered MHCC97H and HepG2 cell apoptosis by downregulating Bcl‑2/Bax expression, and upregulating proapoptotic and apoptotic protein expression via the p53/Bcl2/caspase signaling pathway. JIB‑04 had effects on the inhibition of cell viability and the induction of apoptosis in MHCC97H and HepG2 cells. The underlying mechanism of action of JIB‑04 was associated with the p53/Bcl‑2/caspase signaling pathway. Our findings provide a foundation for understanding the anticancer effect of JIB‑04 on MHCC97H and HepG2 cells, and suggested that JIB‑04 may be a promising therapeutic agent in human liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liao W, Liu J, Liu B, Huang X, Yin Y, Cai D, Li M and Zhu R: JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells. Oncol Rep 40: 3812-3820, 2018.
APA
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D. ... Zhu, R. (2018). JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells. Oncology Reports, 40, 3812-3820. https://doi.org/10.3892/or.2018.6737
MLA
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D., Li, M., Zhu, R."JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells". Oncology Reports 40.6 (2018): 3812-3820.
Chicago
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D., Li, M., Zhu, R."JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells". Oncology Reports 40, no. 6 (2018): 3812-3820. https://doi.org/10.3892/or.2018.6737
Copy and paste a formatted citation
x
Spandidos Publications style
Liao W, Liu J, Liu B, Huang X, Yin Y, Cai D, Li M and Zhu R: JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells. Oncol Rep 40: 3812-3820, 2018.
APA
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D. ... Zhu, R. (2018). JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells. Oncology Reports, 40, 3812-3820. https://doi.org/10.3892/or.2018.6737
MLA
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D., Li, M., Zhu, R."JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells". Oncology Reports 40.6 (2018): 3812-3820.
Chicago
Liao, W., Liu, J., Liu, B., Huang, X., Yin, Y., Cai, D., Li, M., Zhu, R."JIB‑04 induces cell apoptosis via activation of the p53/Bcl‑2/caspase pathway in MHCC97H and HepG2 cells". Oncology Reports 40, no. 6 (2018): 3812-3820. https://doi.org/10.3892/or.2018.6737
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