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Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer

  • Authors:
    • Jia‑Shun Wu
    • Li Li
    • Sha‑Sha Wang
    • Xin Pang
    • Jing‑Biao Wu
    • Su‑Rui Sheng
    • Ya‑Jie Tang
    • Ya‑Ling Tang
    • Min Zheng
    • Xin‑Hua Liang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Stomatology, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, Zhejiang 316021, P.R. China, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, Hubei 430068, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3381-3391
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    Published online on: September 28, 2018
       https://doi.org/10.3892/or.2018.6747
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Abstract

It has previously been demonstrated that autophagy and inflammation act synergistically to promote carcinogenesis. However, the precise roles of autophagy in multistep oral carcinogenesis are still unclear, particularly regarding its association with tumor inflammation. The present study established a 4NQO‑induced oral cancer mouse model and investigated autophagy status in the multistep process of oral carcinogenesis using immunohistochemistry, western blotting and immunofluorescence staining. Furthermore, the number of Gr‑1+CD11b+ myeloid derived suppressor cells (MDSCs) and CD4+ Foxp3+ regulatory T cells (Tregs) during oral carcinogenesis and the association with autophagy status was also examined. The results revealed that the expression of autophagy biomarkers, including dihydrosphingosine 1-phosphate phosphatase LCB3 (LC3B), p62/SQSTM1 (p62) and Beclin 1 increased during 4NQO‑induced carcinogenesis and in human oral cancer. The number of MDSCs and Tregs also increased during oral carcinogenesis. Furthermore, the expression of LC3B and p62 significantly correlated with the accumulation of MDSCs and the expression of Beclin 1 correlated with the increase of Tregs. These data indicated that autophagy may be activated by the tumor inflammation microenvironment during oral carcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Wu JS, Li L, Wang SS, Pang X, Wu JB, Sheng SR, Tang YJ, Tang YL, Zheng M, Liang XH, Liang XH, et al: Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer. Oncol Rep 40: 3381-3391, 2018.
APA
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S. ... Liang, X. (2018). Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer. Oncology Reports, 40, 3381-3391. https://doi.org/10.3892/or.2018.6747
MLA
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S., Tang, Y., Tang, Y., Zheng, M., Liang, X."Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer". Oncology Reports 40.6 (2018): 3381-3391.
Chicago
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S., Tang, Y., Tang, Y., Zheng, M., Liang, X."Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer". Oncology Reports 40, no. 6 (2018): 3381-3391. https://doi.org/10.3892/or.2018.6747
Copy and paste a formatted citation
x
Spandidos Publications style
Wu JS, Li L, Wang SS, Pang X, Wu JB, Sheng SR, Tang YJ, Tang YL, Zheng M, Liang XH, Liang XH, et al: Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer. Oncol Rep 40: 3381-3391, 2018.
APA
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S. ... Liang, X. (2018). Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer. Oncology Reports, 40, 3381-3391. https://doi.org/10.3892/or.2018.6747
MLA
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S., Tang, Y., Tang, Y., Zheng, M., Liang, X."Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer". Oncology Reports 40.6 (2018): 3381-3391.
Chicago
Wu, J., Li, L., Wang, S., Pang, X., Wu, J., Sheng, S., Tang, Y., Tang, Y., Zheng, M., Liang, X."Autophagy is positively associated with the accumulation of myeloid‑derived suppressor cells in 4‑nitroquinoline‑1‑oxide‑induced oral cancer". Oncology Reports 40, no. 6 (2018): 3381-3391. https://doi.org/10.3892/or.2018.6747
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