miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expression

  • Authors:
    • Tianlong Ling
    • Fengrong Yu
    • Hui Cao
  • View Affiliations

  • Published online on: October 3, 2018     https://doi.org/10.3892/or.2018.6765
  • Pages: 3705-3713
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Abstract

Gastrointestinal stromal tumors (GISTs) are the most common type of mesenchymal tumor of the digestive tract. MicroRNAs (miRNAs) are short non-coding RNAs, which control gene expression at a post-transcriptional level. Dysregulated miRNAs are involved in various types of human disease, including cancer. In the present study, it was revealed that miRNA-182 (miR-182) expression was significantly upregulated in human GISTs compared with adjacent normal tissues. Overexpression of miR-182 enhanced GIST-T1 cell growth, with increased proliferation and decreased apoptosis. miR-182 upregulation also promoted colony formation and migration of GIST-T1 cells. In addition, cylindromatosis (CYLD) was identified as a direct target of miR-182. Overexpression of miR-182 suppressed CYLD expression and enhanced downstream nuclear factor (NF)-κB activation. It was also determined that the expression of CYLD was downregulated in association with upregulated miR-182 in human GISTs. In conclusion, these results demonstrated that miR-182 promoted GIST cell growth by negatively regulating CYLD expression. These findings indicated that miR-182 antagonist may be a promising therapeutic strategy for the treatment of human GIST.
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December-2018
Volume 40 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ling T, Yu F and Cao H: miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expression. Oncol Rep 40: 3705-3713, 2018
APA
Ling, T., Yu, F., & Cao, H. (2018). miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expression. Oncology Reports, 40, 3705-3713. https://doi.org/10.3892/or.2018.6765
MLA
Ling, T., Yu, F., Cao, H."miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expression". Oncology Reports 40.6 (2018): 3705-3713.
Chicago
Ling, T., Yu, F., Cao, H."miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expression". Oncology Reports 40, no. 6 (2018): 3705-3713. https://doi.org/10.3892/or.2018.6765