Cancer stem cell biomarkers for head and neck squamous cell carcinoma: A bioinformatic analysis

Xiao,Meng; Liu,Liu; Zhang,Shijian; Yang,Xi; Wang,Yan'an

doi:10.3892/or.2018.6771

Cancer stem cell biomarkers for head and neck squamous cell carcinoma: A bioinformatic analysis

Authors:
- Meng Xiao
- Liu Liu
- Shijian Zhang
- Xi Yang
- Yan'an Wang
View Affiliations

Affiliations: Department of Oral and Maxillofacial‑Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China
Published online on: October 8, 2018 https://doi.org/10.3892/or.2018.6771
Pages: 3843-3851

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Several putative biomarkers have been reported to identify cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC). Herein, we aimed to demonstrate the validity and the underlying relationship for these biomarkers in HNSCC. Bioinformatic analyses for the reported CSC biomarkers of HNSCC were performed based on the TCGA primary HNSCC cohort using the UCSC Xena browser. Targeted strategies for the validated biomarkers were searched and summarized. A total of 27 reported CSC biomarkers for HNSCC were identified and comprehensively evaluated. In regards to the expression pattern of CD44 in HNSCC, the expression patterns for the remaining 26 biomarkers presented 3 different tendencies. We managed to include all the 27 CSC biomarkers for HNSCC into 3 groups. Moreover, the biomarkers in each group indicated distinct clinicopathological features and a different overall survival status for HNSCC patients. The above information suggested the existence of CSC subpopulations in HNSCC. Accordingly, we demonstrated that precisely targeted strategies based on the CSC subgrouping clusters might effectively supplement conventional therapies, and benefit HNSCC patients. Further relevant studies are still necessary to improve treatment strategies for HNSCC based on the CSC area.

View Figures

View References

1	Sun S and Wang Z: Head neck squamous cell carcinoma c-Met⁺ cells display cancer stem cell properties and are responsible for cisplatin-resistance and metastasis. Int J Cancer. 129:2337–2348. 2011. View Article : Google Scholar : PubMed/NCBI
2	Pearson AT, Jackson TL and Nör JE: Modeling head and neck cancer stem cell-mediated tumorigenesis. Cell Mol Life Sci. 73:3279–3289. 2016. View Article : Google Scholar : PubMed/NCBI
3	Leemans CR, Snijders PJF and Brakenhoff RH: The molecular landscape of head and neck cancer. Nat Rev Cancer. 18:269–282. 2018. View Article : Google Scholar : PubMed/NCBI
4	Yu CC, Lo WL, Chen YW, Huang PI, Hsu HS, Tseng LM, Hung SC, Kao SY, Chang CJ and Chiou SH: Bmi-1 regulates snail expression and promotes metastasis ability in head and neck squamous cancer-derived ALDH1 positive cells. J Oncol. 2011:1–16. 2011. View Article : Google Scholar
5	Ringash J: Survivorship and quality of life in head and neck cancer. J Clin Oncol. 33:3322–3327. 2015. View Article : Google Scholar : PubMed/NCBI
6	Prince ME and Ailles LE: Cancer stem cells in head and neck squamous cell cancer. J Clin Oncol. 26:2871–2875. 2008. View Article : Google Scholar : PubMed/NCBI
7	Plaks V, Kong N and Werb Z: The cancer stem cell niche: How essential is the niche in regulating stemness of tumor cells? Cell Stem Cell. 16:225–238. 2015. View Article : Google Scholar : PubMed/NCBI
8	Sayed SI, Dwivedi RC, Katna R, Garg A, Pathak KA, Nutting CM, Rhys-Evans P, Harrington KJ and Kazi R: Implications of understanding cancer stem cell (CSC) biology in head and neck squamous cell cancer. Oral Oncol. 47:237–243. 2011. View Article : Google Scholar : PubMed/NCBI
9	Abbaszadegan MR, Bagheri V, Razavi MS, Momtazi AA, Sahebkar A and Gholamin M: Isolation, identification, and characterization of cancer stem cells (Review). J Cell Physiol. 232:2008–2018. 2017. View Article : Google Scholar : PubMed/NCBI
10	Wolmarans E, Boy SC, Nel S, Mercier AE and Pepper MS: Cancer stem cells in head and neck carcinomas: Identification and possible therapeutic implications. Adv Exp Med Biol. Nov 15–2017.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
11	Shaikh MV, Kala M and Nivsarkar M: CD90 a potential cancer stem cell marker and a therapeutic target. Cancer Biomark. 16:301–307. 2016. View Article : Google Scholar : PubMed/NCBI
12	Zhang Q, Shi S, Yen Y, Brown J, Ta JQ and Le AD: A subpopulation of CD133⁺ cancer stem-like cells characterized in human oral squamous cell carcinoma confer resistance to chemotherapy. Cancer Lett. 289:151–160. 2010. View Article : Google Scholar : PubMed/NCBI
13	Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF and Ailles LE: Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci USA. 104:973–978. 2007. View Article : Google Scholar : PubMed/NCBI
14	Zhou C and Sun B: The prognostic role of the cancer stem cell marker aldehyde dehydrogenase 1 in head and neck squamous cell carcinomas: A meta-analysis. Oral Oncol. 50:1144–1148. 2014. View Article : Google Scholar : PubMed/NCBI
15	Pozzi V, Sartini D, Rocchetti R, Santarelli A, Rubini C, Morganti S, Giuliante R, Calabrese S, Di Ruscio G, Orlando F, et al: Identification and characterization of cancer stem cells from head and neck squamous cell carcinoma cell lines. Cell Physiol Biochem. 36:784–798. 2015. View Article : Google Scholar : PubMed/NCBI
16	Sun W, Bunn P, Jin C, Little P, Zhabotynsky V, Perou CM, Hayes DN, Chen M and Lin DY: The association between copy number aberration, DNA methylation and gene expression in tumor samples. Nucleic Acids Res. 46:3009–3018. 2018. View Article : Google Scholar : PubMed/NCBI
17	Marzouka NA, Eriksson P, Rovira C, Liedberg F, Sjödahl G and Höglund M: A validation and extended description of the Lund taxonomy for urothelial carcinoma using the TCGA cohort. Sci Rep. 8:37372018. View Article : Google Scholar : PubMed/NCBI
18	Zhu J, Sanborn JZ, Benz S, Szeto C, Hsu F, Kuhn RM, Karolchik D, Archie J, Lenburg ME, Esserman LJ, et al: The UCSC cancer genomics browser. Nat Methods. 6:239–240. 2009. View Article : Google Scholar : PubMed/NCBI
19	Kokko LL, Hurme S, Maula SM, Alanen K, Grénman R, Kinnunen I and Ventelä S: Significance of site-specific prognosis of cancer stem cell marker CD44 in head and neck squamous-cell carcinoma. Oral Oncol. 47:510–516. 2011. View Article : Google Scholar : PubMed/NCBI
20	Judd NP, Winkler AE, Murillo-Sauca O, Brotman JJ, Law JH, Lewis JS Jr, Dunn GP, Bui JD, Sunwoo JB and Uppaluri R: ERK1/2 regulation of CD44 modulates oral cancer aggressiveness. Cancer Res. 72:365–374. 2012. View Article : Google Scholar : PubMed/NCBI
21	Major AG, Pitty LP and Farah CS: Cancer stem cell markers in head and neck squamous cell carcinoma. Stem Cells Int. 2013:3194892013. View Article : Google Scholar : PubMed/NCBI
22	Kiang A, Yu MA and Ongkeko WM: Progress and pitfalls in the identification of cancer stem cell-targeting therapies in head and neck squamous cell carcinoma. Curr Med Chem. 19:6056–6064. 2012. View Article : Google Scholar : PubMed/NCBI
23	Gilormini M, Wozny AS, Battiston-Montagne P, Ardail D, Alphonse G and Rodriguez-Lafrasse C: Isolation and characterization of a head and neck squamous cell carcinoma subpopulation having stem cell characteristics. J Vis Exp. 2016:e539582016.
24	Zimmerer RM, Ludwig N, Kampmann A, Bittermann G, Spalthoff S, Jungheim M, Gellrich NC and Tavassol F: CD24⁺ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo. Microvasc Res. 112:101–108. 2017. View Article : Google Scholar : PubMed/NCBI
25	Martens-de Kemp SR, Brink A, Stigter-Van Walsum M, Damen JM, Rustenburg F, Wu T, Van Wieringen WN, Schuurhuis GJ, Braakhuis BJ, Slijper M, et al: CD98 marks a subpopulation of head and neck squamous cell carcinoma cells with stem cell properties. Stem Cell Res. 10:477–488. 2013. View Article : Google Scholar : PubMed/NCBI
26	Waldron NN, Barsky SH, Dougherty PR and Vallera DA: A bispecific EpCAM/CD133-targeted toxin is effective against carcinoma. Target Oncol. 9:239–249. 2014. View Article : Google Scholar : PubMed/NCBI
27	Lim YC, Kang HJ and Moon JH: c-Met pathway promotes self-renewal and tumorigenecity of head and neck squamous cell carcinoma stem-like cell. Oral Oncol. 50:633–639. 2014. View Article : Google Scholar : PubMed/NCBI
28	Yan M, Yang X, Wang L, Clark D, Zuo H, Ye D, Chen W and Zhang P: Plasma membrane proteomics of tumor spheres identify CD166 as a novel marker for cancer stem-like cells in head and neck squamous cell carcinoma. Mol Cell Proteomics. 12:3271–3284. 2013. View Article : Google Scholar : PubMed/NCBI
29	Lee SH, Do SI, Lee HJ, Kang HJ, Koo BS and Lim YC: Notch1 signaling contributes to stemness in head and neck squamous cell carcinoma. Lab Invest. 96:508–516. 2016. View Article : Google Scholar : PubMed/NCBI
30	Fukusumi T, Ishii H, Konno M, Yasui T, Nakahara S, Takenaka Y, Yamamoto Y, Nishikawa S, Kano Y, Ogawa H, et al: CD10 as a novel marker of therapeutic resistance and cancer stem cells in head and neck squamous cell carcinoma. Br J Cancer. 111:506–514. 2014. View Article : Google Scholar : PubMed/NCBI
31	Yang CC, Zhu LF, Xu XH, Ning TY, Ye JH and Liu LK: Membrane Type 1 Matrix metalloproteinase induces an epithelial to mesenchymal transition and cancer stem cell-like properties in SCC9 cells. BMC Cancer. 13:1712013. View Article : Google Scholar : PubMed/NCBI
32	Chou MY, Hu FW, Yu CH and Yu CC: Sox2 expression involvement in the oncogenicity and radiochemoresistance of oral cancer stem cells. Oral Oncol. 51:31–39. 2015. View Article : Google Scholar : PubMed/NCBI
33	Chiou SH, Yu CC, Huang CY, Lin SC, Liu CJ, Tsai TH, Chou SH, Chien CS, Ku HH and Lo JF: Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma. Clin Cancer Res. 14:4085–4095. 2008. View Article : Google Scholar : PubMed/NCBI
34	Chen D, Wu M, Li Y, Chang I, Yuan Q, Ekimyan-Salvo M, Deng P, Yu B, Yu Y, Dong J, et al: Targeting BMI1⁺ cancer stem cells overcomes chemoresistance and inhibits metastases in squamous cell carcinoma. Cell Stem Cell. 20:621–634.e6. 2017. View Article : Google Scholar : PubMed/NCBI
35	Oliveira-Costa JP, Oliveira LR, da Silveira GG, Soave DF, Soares FA and Ribeiro-Silva A: Topoisomerase expression in oral squamous cell carcinoma: Relationship with cancer stem cells profiles and lymph node metastasis. J Oral Pathol Med. 41:762–768. 2012. View Article : Google Scholar : PubMed/NCBI
36	Li Z, Wang Y, Zhu Y, Yuan C, Wang D, Zhang W, Qi B, Qiu J, Song X, Ye J, et al: The Hippo transducer TAZ promotes epithelial to mesenchymal transition and cancer stem cell maintenance in oral cancer. Mol Oncol. 9:1091–1105. 2015. View Article : Google Scholar : PubMed/NCBI
37	Lee CR, Lee SH, Rigas NK, Kim RH, Kang MK, Park NH and Shin KH: Elevated expression of JMJD6 is associated with oral carcinogenesis and maintains cancer stemness properties. Carcinogenesis. 37:119–128. 2016. View Article : Google Scholar : PubMed/NCBI
38	Shen B, Dong P, Li D and Gao S: Expression and function of ABCG2 in head and neck squamous cell carcinoma and cell lines. Exp Ther Med. 2:1151–1157. 2011. View Article : Google Scholar : PubMed/NCBI
39	Lee DG, Lee JH, Choi BK, Kim MJ, Kim SM, Kim KS, Chang K, Park SH, Bae YS and Kwon BS: H⁺-myo-inositol transporter SLC2A13 as a potential marker for cancer stem cells in an oral squamous cell carcinoma. Curr Cancer Drug Targets. 11:966–975. 2011. View Article : Google Scholar : PubMed/NCBI
40	Wu MJ, Jan CI, Tsay YG, Yu YH, Huang CY, Lin SC, Liu CJ, Chen YS, Lo JF and Yu CC: Elimination of head and neck cancer initiating cells through targeting glucose regulated protein78 signaling. Mol Cancer. 9:2832010. View Article : Google Scholar : PubMed/NCBI
41	Da Cruz Paula A and Lopes C: Implications of different cancer stem cell phenotypes in breast cancer. Anticancer Res. 37:2173–2183. 2017. View Article : Google Scholar : PubMed/NCBI
42	Eun K, Ham SW and Kim H: Cancer stem cell heterogeneity: Origin and new perspectives on CSC targeting. BMB Rep. 50:117–125. 2017. View Article : Google Scholar : PubMed/NCBI
43	Han J, Fujisawa T, Husain SR and Puri RK: Identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma. BMC Cancer. 14:1732014. View Article : Google Scholar : PubMed/NCBI