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Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation

  • Authors:
    • Jingling Jin
    • Junliang Jin
    • Sarah E. Woodfield
    • Roma H. Patel
    • Nan Ge Jin
    • Yan Shi
    • Bin Liu
    • Wenjing Sun
    • Xiangmei Chen
    • Yang Yu
    • Sanjeev A. Vasudevan
  • View Affiliations / Copyright

    Affiliations: Divisions of Pediatric Surgery and Surgical Research, Michael E. DeBakey, Department of Surgery, Baylor College of Medicine, Texas Children's Hospital, Houston, TX 77030, USA, Carnegie Vanguard High School, Houston, TX 77019, USA, Ruiz Department of Ophthalmology and Visual Science, University of Texas Health Science Center at Houston, Houston, TX 77030, USA, Department of Pediatrics‑Oncology, Baylor College of Medicine, Texas Children's Hospital, Houston, TX 77030, USA, Department of Microbiology & Infectious Disease Center, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 143-153
    |
    Published online on: October 15, 2018
       https://doi.org/10.3892/or.2018.6793
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Abstract

Hepatoblastoma is the most common malignant liver tumor in children. Since it is often unresectable and exhibits drug resistance, the treatment of advanced hepatoblastoma is challenging. The orphan nuclear receptor liver receptor homolog‑1 (LRH‑1) serves prominent roles in malignancy; however, to the best of our knowledge, the role of LRH‑1 in hepatoblastoma remains unknown. In the present study, human hepatoblastoma cell lines were analyzed; the mRNA and protein expression levels of LRH‑1 were significantly higher in HepG2 and HuH6 cells compared with those in HepT1 cells and control THLE‑2 cells. Knockdown of LRH‑1 resulted in decreased HepG2 and HuH6 cell proliferation via downregulation of cyclin D1 (CCND1) and c‑Myc. Furthermore, treatment with an LRH‑1 antagonist (LRA) inhibited the proliferation and colony formation of cell lines in a dose‑dependent manner, and induced cell cycle arrest at G1 phase through inhibition of CCND1 expression. Finally, LRA treatment enhanced the cytotoxic effects of doxorubicin on hepatoblastoma cells. Collectively, these findings suggested that LRH‑1 may have an important role in the progression of hepatoblastoma and implicated LRA as a novel, potential therapeutic agent for the treatment of hepatoblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Jin J, Jin J, Woodfield SE, Patel RH, Jin NG, Shi Y, Liu B, Sun W, Chen X, Yu Y, Yu Y, et al: Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation. Oncol Rep 41: 143-153, 2019.
APA
Jin, J., Jin, J., Woodfield, S.E., Patel, R.H., Jin, N.G., Shi, Y. ... Vasudevan, S.A. (2019). Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation. Oncology Reports, 41, 143-153. https://doi.org/10.3892/or.2018.6793
MLA
Jin, J., Jin, J., Woodfield, S. E., Patel, R. H., Jin, N. G., Shi, Y., Liu, B., Sun, W., Chen, X., Yu, Y., Vasudevan, S. A."Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation". Oncology Reports 41.1 (2019): 143-153.
Chicago
Jin, J., Jin, J., Woodfield, S. E., Patel, R. H., Jin, N. G., Shi, Y., Liu, B., Sun, W., Chen, X., Yu, Y., Vasudevan, S. A."Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation". Oncology Reports 41, no. 1 (2019): 143-153. https://doi.org/10.3892/or.2018.6793
Copy and paste a formatted citation
x
Spandidos Publications style
Jin J, Jin J, Woodfield SE, Patel RH, Jin NG, Shi Y, Liu B, Sun W, Chen X, Yu Y, Yu Y, et al: Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation. Oncol Rep 41: 143-153, 2019.
APA
Jin, J., Jin, J., Woodfield, S.E., Patel, R.H., Jin, N.G., Shi, Y. ... Vasudevan, S.A. (2019). Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation. Oncology Reports, 41, 143-153. https://doi.org/10.3892/or.2018.6793
MLA
Jin, J., Jin, J., Woodfield, S. E., Patel, R. H., Jin, N. G., Shi, Y., Liu, B., Sun, W., Chen, X., Yu, Y., Vasudevan, S. A."Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation". Oncology Reports 41.1 (2019): 143-153.
Chicago
Jin, J., Jin, J., Woodfield, S. E., Patel, R. H., Jin, N. G., Shi, Y., Liu, B., Sun, W., Chen, X., Yu, Y., Vasudevan, S. A."Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation". Oncology Reports 41, no. 1 (2019): 143-153. https://doi.org/10.3892/or.2018.6793
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