MicroRNA‑494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression

  • Authors:
    • Kun Han
    • Zhao Jian Li
    • Peng Sun
  • View Affiliations

  • Published online on: October 25, 2018     https://doi.org/10.3892/or.2018.6823
  • Pages: 351-360
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Abstract

The aim of the present study was to analyze the possible association between microRNA‑494 (miR‑494) and cell proliferation in glioma cancer. Firstly, the expression of miR‑494 was revealed to be upregulated in patients with glioma, compared with the normal group. Next, anti‑miR‑494 mimics were used to decrease the expression of miR‑494 in glioma cancer cells, which subsequently induced apoptosis, and inhibited cell growth and migration. Downregulation of miR‑494 expression induced phosphatase and tensin homolog (PTEN) and suppressed the protein kinase B/mechanistic target of rapamycin pathway (Akt/mTOR) pathway in glioma cancer cells. By contrast, overexpression of miR‑494 by miR‑494 mimics promoted cell growth and migration, and suppressed the apoptosis of glioma cancer via the Akt/mTOR pathway by PTEN expression. Furthermore, a PTEN inhibitor was used to attenuate the function of miR‑494 in glioma cancer autophagy through Akt/mTOR pathway. The promotion of PTEN promoted the function of anti‑miR‑494 on glioma cancer cell growth through Akt/mTOR pathway. Collectively, these results demonstrate that the effect of miRNA‑494 on the proliferation and migration glioma cancer cells was mediated through Akt/mTOR pathway by PTEN expression.
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January-2019
Volume 41 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Han K, Li ZJ and Sun P: MicroRNA‑494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression. Oncol Rep 41: 351-360, 2019
APA
Han, K., Li, Z.J., & Sun, P. (2019). MicroRNA‑494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression. Oncology Reports, 41, 351-360. https://doi.org/10.3892/or.2018.6823
MLA
Han, K., Li, Z. J., Sun, P."MicroRNA‑494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression". Oncology Reports 41.1 (2019): 351-360.
Chicago
Han, K., Li, Z. J., Sun, P."MicroRNA‑494 promotes the proliferation and migration of human glioma cancer cells through the protein kinase B/mechanistic target of rapamycin pathway by phosphatase and tensin homolog expression". Oncology Reports 41, no. 1 (2019): 351-360. https://doi.org/10.3892/or.2018.6823