Ubiquitin‑specific protease 7 promotes osteosarcoma cell metastasis by inducing epithelial‑mesenchymal transition

Zeng,Qiuming; Li,Zongyuan; Zhao,Xi; Guo,Liang; Yu,Chao; Qin,Jun; Zhang,Shiqiong; Zhang,Yongle; Yang,Xuhui

doi:10.3892/or.2018.6835

Ubiquitin‑specific protease 7 promotes osteosarcoma cell metastasis by inducing epithelial‑mesenchymal transition

Authors:
- Qiuming Zeng
- Zongyuan Li
- Xi Zhao
- Liang Guo
- Chao Yu
- Jun Qin
- Shiqiong Zhang
- Yongle Zhang
- Xuhui Yang
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Affiliations: Department of Orthopaedics, University‑Town Hospital of Chongqing Medical University, Chongqing, Sichuan 401331, P.R. China, Department of Orthopaedics, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China
Published online on: October 31, 2018 https://doi.org/10.3892/or.2018.6835
Pages: 543-551

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Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumour among adolescents and young adults; however, its molecular pathogenesis has not been completely elucidated. Ubiquitin‑specific protease 7 (USP7), a member of the deubiquitinating enzyme family, plays a role in the malignancy process of various cancer types by targeting the key oncoprotein; however, its biological function and mechanism in OS have not been elucidated. The present study demonstrated that USP7 expression in OS tumour tissues was markedly higher than that in the paired surrounding tissues, and high USP7 expression was positively correlated with the TNM stage and metastasis in patients with OS. Next, biological function assays demonstrated that USP7 knockdown markedly inhibited OS cell migration and invasion, whereas USP7 overexpression enhanced it. Notably, USP7 can directly bind with β‑catenin to activate the Wnt/β‑catenin signalling pathway and induce epithelial‑mesenchymal transition (EMT) of OS cells. Overall, USP7 overexpression could promote OS cell metastasis by activating the Wnt/β‑catenin signalling pathway by inducing EMT, suggesting that USP7 is a potential therapeutic target for OS.

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