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Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer

  • Authors:
    • Huaping Zhou
    • Allen Chen
    • Jianfei Shen
    • Xiaoxue Zhang
    • Min Hou
    • Jin Li
    • Jingyi Chen
    • Huan Zou
    • Yingfei Zhang
    • Qianren Deng
    • Kelin She
    • Xiaoshun Shi
    • Jianxing He
  • View Affiliations / Copyright

    Affiliations: Southern Medical University, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Mathematics, University of California, Berkeley, CA 94720, USA, Department of Cardiothoracic Surgery, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang 317000, P.R. China, Department of the Second Division of Cardiovascular Medicine, Guangdong No. 2 Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Pathology, Affiliated Cancer Hospital andInstitute of Guangzhou Medical University, Guangzhou, Guangdong 510530, P.R. China, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou, Guangdong 510120, P.R. China, SaiQing Biotech Co. Ltd., Guangzhou, Guangdong 510599, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 15-26
    |
    Published online on: October 31, 2018
       https://doi.org/10.3892/or.2018.6839
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Abstract

Recently, LOC285194 has shown a potential tumor-suppressor function in several types of human cancers, but its function in non-small cell lung cancer (NSCLC) remains unknown. This study intended to investigate the biological role of LOC285194 and its clinical significance in NSCLC. LOC285194 was detected by qRT-PCR, and its correlation with clinicopathological features of NSCLC was analyzed. The expression of LOC285194 was knocked down or ectopically expressed in lung cancer cells (A549 and H1299) and tumor cell growth, migration and invasion in vitro were investigated. In addition, the interaction of LOC285194 and target proteins was assessed by RNA pulldown and RNA immunoprecipitation in vitro. The results revealed that the expression of LOC285194 was significantly lower in tumor tissues when compared with the corresponding non-tumor tissues (P<0.001). Its expression was correlated with the tumor size (P=0.027). Kaplan-Meier analysis revealed that patients with lower LOC285194 expression had worse disease-free survival and overall survival rates (P<0.05). RNA protein interaction analysis revealed that p53 was the direct binding target of LOC285194 in NSCLC. Bioinformatics analyses suggested that depletion of LOC285149 could affect its antitumor function through the KRAS/BRAF/SMEK pathway. Our findings indicated that LOC285194 was a novel non-coding prognostic indicator and contributed to tumor suppression by targeting p53 in NSCLC, suggesting that it may be a non-coding target for NSCLC gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou H, Chen A, Shen J, Zhang X, Hou M, Li J, Chen J, Zou H, Zhang Y, Deng Q, Deng Q, et al: Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncol Rep 41: 15-26, 2019.
APA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J. ... He, J. (2019). Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncology Reports, 41, 15-26. https://doi.org/10.3892/or.2018.6839
MLA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41.1 (2019): 15-26.
Chicago
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41, no. 1 (2019): 15-26. https://doi.org/10.3892/or.2018.6839
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou H, Chen A, Shen J, Zhang X, Hou M, Li J, Chen J, Zou H, Zhang Y, Deng Q, Deng Q, et al: Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncol Rep 41: 15-26, 2019.
APA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J. ... He, J. (2019). Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncology Reports, 41, 15-26. https://doi.org/10.3892/or.2018.6839
MLA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41.1 (2019): 15-26.
Chicago
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41, no. 1 (2019): 15-26. https://doi.org/10.3892/or.2018.6839
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