Open Access

Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer

  • Authors:
    • Huaping Zhou
    • Allen Chen
    • Jianfei Shen
    • Xiaoxue Zhang
    • Min Hou
    • Jin Li
    • Jingyi Chen
    • Huan Zou
    • Yingfei Zhang
    • Qianren Deng
    • Kelin She
    • Xiaoshun Shi
    • Jianxing He
  • View Affiliations

  • Published online on: October 31, 2018     https://doi.org/10.3892/or.2018.6839
  • Pages: 15-26
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recently, LOC285194 has shown a potential tumor-suppressor function in several types of human cancers, but its function in non-small cell lung cancer (NSCLC) remains unknown. This study intended to investigate the biological role of LOC285194 and its clinical significance in NSCLC. LOC285194 was detected by qRT-PCR, and its correlation with clinicopathological features of NSCLC was analyzed. The expression of LOC285194 was knocked down or ectopically expressed in lung cancer cells (A549 and H1299) and tumor cell growth, migration and invasion in vitro were investigated. In addition, the interaction of LOC285194 and target proteins was assessed by RNA pulldown and RNA immunoprecipitation in vitro. The results revealed that the expression of LOC285194 was significantly lower in tumor tissues when compared with the corresponding non-tumor tissues (P<0.001). Its expression was correlated with the tumor size (P=0.027). Kaplan-Meier analysis revealed that patients with lower LOC285194 expression had worse disease-free survival and overall survival rates (P<0.05). RNA protein interaction analysis revealed that p53 was the direct binding target of LOC285194 in NSCLC. Bioinformatics analyses suggested that depletion of LOC285149 could affect its antitumor function through the KRAS/BRAF/SMEK pathway. Our findings indicated that LOC285194 was a novel non-coding prognostic indicator and contributed to tumor suppression by targeting p53 in NSCLC, suggesting that it may be a non-coding target for NSCLC gene therapy.
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January-2019
Volume 41 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhou H, Chen A, Shen J, Zhang X, Hou M, Li J, Chen J, Zou H, Zhang Y, Deng Q, Deng Q, et al: Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncol Rep 41: 15-26, 2019
APA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J. ... He, J. (2019). Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer. Oncology Reports, 41, 15-26. https://doi.org/10.3892/or.2018.6839
MLA
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41.1 (2019): 15-26.
Chicago
Zhou, H., Chen, A., Shen, J., Zhang, X., Hou, M., Li, J., Chen, J., Zou, H., Zhang, Y., Deng, Q., She, K., Shi, X., He, J."Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer". Oncology Reports 41, no. 1 (2019): 15-26. https://doi.org/10.3892/or.2018.6839