Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
January-2019 Volume 41 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2019 Volume 41 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Fascin is involved in cancer cell invasion and is regulated by stromal factors

  • Authors:
    • Xianglan Zhang
    • Il‑Hoon Cho
    • Ji Hyeon Park
    • Min Kyeong Lee
    • Young Sun Hwang
  • View Affiliations / Copyright

    Affiliations: Yonsei University, College of Dentistry, Seoul 03722, Republic of Korea, Departments of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam, Gyunggi‑Do 13135, Republic of Korea, Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Gyunggi‑Do 13135, Republic of Korea
  • Pages: 465-474
    |
    Published online on: November 2, 2018
       https://doi.org/10.3892/or.2018.6847
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The tumor microenvironment plays an important role in cancer growth, invasion and metastasis. The stroma surrounding a tumor is known to contain a variety of factors that can increase angiogenesis, cancer growth and tumor progression. The aim of the present study was to determine the role of fascin in cancer growth and invasion and identify stromal factors involved in cancer progression. A fascin‑depleted cell line (fascindep) was used to observe the role of fascin in cancer invasion. Compared with wild‑type Mock cells, cancer cell invasion in Matrigel‑coated Transwell and three‑dimensional (3D) culture system were reduced by fascin depletion. Tumor cell growth in vivo was also significantly reduced in mice injected with fascindep cells. Notably, fascin expression was increased during Transwell invasion with Matrigel compared to Transwell invasion without Matrigel. TGF‑β1, EGF and IL‑1β significantly stimulated fascin expression. Such increased expression of fascin was also observed in cultured cells using conditioned media (CM) from cancer‑associated fibroblasts (CAFs). However, no significant change in fascin expression was observed using CM from normal fibroblasts (NFs). Stimulated expression of fascin by Matrigel and CAFs was reduced by biological specific inhibitor of TGF‑β1, EGF and IL‑1β. Compared with wild‑type Mock cells, the fascindep cell line showed low RhoA and NF‑κB activity, suggesting that RhoA and NF‑κB signals are involved in fascin expression. In conclusion, stromal factors are involved in cancer invasion and progression by activating intracellular signaling of cancer cells to increase fascin expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Guan X: Cancer metastases: Challenges and opportunities. Acta Pharm Sin B. 5:402–418. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Eddy RJ, Weidmann MD, Sharma VP and Condeelis JS: Tumor cell invadopodia: Invasive protrusions that orchestrate metastasis. Trends Cell Biol. 27:595–607. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Brabletz T, Kalluri R, Nieto MA and Weinberg RA: EMT in cancer. Nat Rev Cancer. 18:128–134. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Gould CM and Courtneidge SA: Regulation of invadopodia by the tumor microenvironment. Cell Adh Migr. 8:226–235. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Li A, Dawson JC, Forero-Vargas M, Spence HJ, Yu X, König I, Anderson K and Machesky LM: The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion. Curr Biol. 20:339–345. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung DW, Jeon NK, Choi YW, Lee EJ and Kim J: Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma. Cancer Lett. 275:61–71. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Toth M, Sohail A and Fridman R: Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography. Methods Mol Biol. 878:121–135. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Hwang YS, Park KK and Chung WY: Stromal transforming growth factor-beta 1 is crucial for reinforcing the invasive potential of low invasive cancer. Arch Oral Biol. 59:687–694. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Bowden ET, Coopman PJ and Mueller SC: Invadopodia: Unique methods for measurement of extracellular matrix degradation in vitro. Methods Cell Biol. 63:613–627. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Hwang YS, Park KK, Cha IH, Kim J and Chung WY: Role of insulin-like growth factor-II mRNA-binding protein-3 in invadopodia formation and the growth of oral squamous cell carcinoma in athymic nude mice. Head Neck. 34:1329–1339. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Hodge JC, Bub J, Kaul S, Kajdacsy-Balla A and Lindholm PF: Requirement of RhoA activity for increased nuclear factor kappaB activity and PC-3 human prostate cancer cell invasion. Cancer Res. 63:1359–1364. 2003.PubMed/NCBI

12 

Struckhoff AP, Rana MK and Worthylake RA: RhoA can lead the way in tumor cell invasion and metastasis. Front Biosci (Landmark Ed). 16:1915–1926. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Bremnes RM, Dønnem T, Al-Saad S, Al-Shibli K, Andersen S, Sirera R, Camps C, Marinez I and Busund LT: The role of tumor stroma in cancer progression and prognosis: Emphasis on carcinoma-associated fibroblasts and non-small cell lung cancer. J Thorac Oncol. 6:209–217. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Shiga K, Hara M, Nagasaki T, Sato T, Takahashi H and Takeyama H: Cancer-associated fibroblasts: Their characteristics and their roles in tumor growth. Cancers. 7:2443–2458. 2015. View Article : Google Scholar : PubMed/NCBI

15 

De Wever O and Mareel M: Role of tissue stroma in cancer cell invasion. J Pathol. 200:429–447. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Li J, Jia Z, Kong J, Zhang F, Fang S, Li X, Li W, Yang X, Luo Y, Lin B, et al: Carcinoma-associated fibroblasts lead the invasion of salivary gland adenoid cystic carcinoma cells by creating an invasive track. PLoS One. 11:e01502472016. View Article : Google Scholar : PubMed/NCBI

17 

Wen S, Niu Y, Yeh S and Chang C: BM-MSCs promote prostate cancer progression via the conversion of normal fibroblasts to cancer-associated fibroblasts. Int J Oncol. 47:719–727. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Taniwaki K, Fukamachi H, Komori K, Ohtake Y, Nonaka T, Sakamoto T, Shiomi T, Okada Y, Itoh T, Itohara S, et al: Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice. Cancer Res. 67:4311–4319. 2007. View Article : Google Scholar : PubMed/NCBI

19 

van Beers EH and Nederlof PM: Array-CGH and breast cancer. Breast Cancer Res. 8:2102006. View Article : Google Scholar : PubMed/NCBI

20 

Al-Alwan M, Olabi S, Ghebeh H, Barhoush E, Tulbah A, Al-Tweigeri T, Ajarim D and Adra C: Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One. 6:e273392011. View Article : Google Scholar : PubMed/NCBI

21 

Huang FK, Han S, Xing B, Huang J, Liu B, Bordeleau F, Reinhart-King CA, Zhang JJ and Huang XY: Targeted inhibition of fascin function blocks tumour invasion and metastatic colonization. Nat Commun. 6:74652015. View Article : Google Scholar : PubMed/NCBI

22 

Min KW, Chae SW, Kim DH, Do SI, Kim K, Lee HJ, Sohn JH, Pyo JS, Kim DH, Oh SJ, et al: Fascin expression predicts an aggressive clinical course in patients with advanced breast cancer. Oncol Lett. 10:121–130. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Darnel AD, Behmoaram E, Vollmer RT, Corcos J, Bijian K, Sircar K, Su J, Jiao J, Alaoui-Jamali MA and Bismar TA: Fascin regulates prostate cancer cell invasion and is associated with metastasis and biochemical failure in prostate cancer. Clin Cancer Res. 15:1376–1383. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Wang CQ, Tang CH, Chang HT, Li XN, Zhao YM, Su CM, Hu GN, Zhang T, Sun XX, Zeng Y, et al: Fascin-1 as a novel diagnostic marker of triple-negative breast cancer. Cancer Med. 5:1983–1988. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Takikita M, Hu N, Shou JZ, Giffen C, Wang QH, Wang C, Hewitt SM and Taylor PR: Fascin and CK4 as biomarkers for esophageal squamous cell carcinoma. Anticancer Res. 31:945–952. 2011.PubMed/NCBI

26 

Li L, Cao F, Liu B, Luo X, Ma X and Hu Z: TGF-β induces fascin expression in gastric cancer via phosphorylation of smad3 linker area. Am J Cancer Res. 5:1890–1896. 2015.PubMed/NCBI

27 

Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S and Yang S: GATA3 transcription factor abrogates Smad4 transcription factor-mediated fascin overexpression, invadopodium formation, and breast cancer cell invasion. J Biol Chem. 288:36971–36982. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Ghebeh H, Al-Khaldi S, Olabi S, Al-Dhfyan A, Al-Mohanna F, Barnawi R, Tulbah A, Al-Tweigeri T, Ajarim D and Al-Alwan M: Fascin is involved in the chemotherapeutic resistance of breast cancer cells predominantly via the PI3K/Akt pathway. Br J Cancer. 111:1552–1561. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Hashimoto Y, Loftis DW and Adams JC: Fascin-1 promoter activity is regulated by CREB and the aryl hydrocarbon receptor in human carcinoma cells. PLoS One. 4:e51302009. View Article : Google Scholar : PubMed/NCBI

30 

Langhans SA: Three-dimensional in vitro cell culture models in drug discovery and drug repositioning. Front Pharmacol. 9:62018. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Cho IH, Park JH, Lee MK and Hwang YS: Fascin is involved in cancer cell invasion and is regulated by stromal factors. Oncol Rep 41: 465-474, 2019.
APA
Zhang, X., Cho, I., Park, J.H., Lee, M.K., & Hwang, Y.S. (2019). Fascin is involved in cancer cell invasion and is regulated by stromal factors. Oncology Reports, 41, 465-474. https://doi.org/10.3892/or.2018.6847
MLA
Zhang, X., Cho, I., Park, J. H., Lee, M. K., Hwang, Y. S."Fascin is involved in cancer cell invasion and is regulated by stromal factors". Oncology Reports 41.1 (2019): 465-474.
Chicago
Zhang, X., Cho, I., Park, J. H., Lee, M. K., Hwang, Y. S."Fascin is involved in cancer cell invasion and is regulated by stromal factors". Oncology Reports 41, no. 1 (2019): 465-474. https://doi.org/10.3892/or.2018.6847
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Cho IH, Park JH, Lee MK and Hwang YS: Fascin is involved in cancer cell invasion and is regulated by stromal factors. Oncol Rep 41: 465-474, 2019.
APA
Zhang, X., Cho, I., Park, J.H., Lee, M.K., & Hwang, Y.S. (2019). Fascin is involved in cancer cell invasion and is regulated by stromal factors. Oncology Reports, 41, 465-474. https://doi.org/10.3892/or.2018.6847
MLA
Zhang, X., Cho, I., Park, J. H., Lee, M. K., Hwang, Y. S."Fascin is involved in cancer cell invasion and is regulated by stromal factors". Oncology Reports 41.1 (2019): 465-474.
Chicago
Zhang, X., Cho, I., Park, J. H., Lee, M. K., Hwang, Y. S."Fascin is involved in cancer cell invasion and is regulated by stromal factors". Oncology Reports 41, no. 1 (2019): 465-474. https://doi.org/10.3892/or.2018.6847
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team