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Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

  • Authors:
    • Sayaka Kobayashi
    • Masanao Saio
    • Toshio Fukuda
    • Kiminori Kimura
    • Junko Hirato
    • Tetsunari Oyama
  • View Affiliations / Copyright

    Affiliations: Laboratory of Histopathology and Cytopathology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Gunma 371-8514, Japan, Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Disease Center, Komagome Hospital, Tokyo 113-8677, Japan, Department of Pathology, Gunma University Hospital, Gunma 371-8511, Japan
    Copyright: © Kobayashi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 133-142
    |
    Published online on: November 2, 2018
       https://doi.org/10.3892/or.2018.6848
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Abstract

Nuclear size and shape are important components in the diagnosis of pathological specimens. However, a qualitative evaluation is typically applied rather than a quantitative evaluation technique. In the present study, we sought to evaluate the nuclear morphological characteristics of lung adenocarcinoma using whole-slide imaging (WSI) and computer-assisted image analysis (IA). We evaluated the nuclear characteristics of 106 cases of surgically resected lung adenocarcinoma according to Feulgen staining and immunohistochemistry (IHC) for the inner nuclear membrane protein emerin. According to the Feulgen reaction, although the nuclear area (size) of the carcinoma cells was correlated with the nuclear perimeter (NP) (R=0.8973), the nuclear staining intensity of carcinoma cells was not correlated with the nuclear area. Using emerin IHC, we used IA software that was able to detect both the NP and the emerin-stained nuclear membrane length (ENML) in the nucleus, and found that the more nuclei exhibited a longer ENML relative to the NP, the more nuclear grooves and intranuclear cytoplasmic inclusions were present. In addition, the nuclear area was correlated with the percentage of nuclei that had a longer ENML compared to the NP against the total nuclei (R=0.7759). Furthermore, the emerin low expression group showed an enlarged nuclear area (P=0.0264), elongated NP (P=0.0091), and lower shape factor (P=0.0486) compared with the normal emerin expression group. Our data indicated the usefulness of WSI and IA for pathological specimen analysis. In addition, this study is the first to report that the low expression of emerin in cancer cell results in an oval shape of nuclei and nuclear enlargement in clinical samples.
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Copy and paste a formatted citation
Spandidos Publications style
Kobayashi S, Saio M, Fukuda T, Kimura K, Hirato J and Oyama T: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncol Rep 41: 133-142, 2019.
APA
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., & Oyama, T. (2019). Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncology Reports, 41, 133-142. https://doi.org/10.3892/or.2018.6848
MLA
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., Oyama, T."Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma". Oncology Reports 41.1 (2019): 133-142.
Chicago
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., Oyama, T."Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma". Oncology Reports 41, no. 1 (2019): 133-142. https://doi.org/10.3892/or.2018.6848
Copy and paste a formatted citation
x
Spandidos Publications style
Kobayashi S, Saio M, Fukuda T, Kimura K, Hirato J and Oyama T: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncol Rep 41: 133-142, 2019.
APA
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., & Oyama, T. (2019). Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncology Reports, 41, 133-142. https://doi.org/10.3892/or.2018.6848
MLA
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., Oyama, T."Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma". Oncology Reports 41.1 (2019): 133-142.
Chicago
Kobayashi, S., Saio, M., Fukuda, T., Kimura, K., Hirato, J., Oyama, T."Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma". Oncology Reports 41, no. 1 (2019): 133-142. https://doi.org/10.3892/or.2018.6848
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