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Article

Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1

  • Authors:
    • Anaz Mohammed
    • Caixin Zhang
    • Shuwen Zhang
    • Qin Shen
    • Jieying Li
    • Zhiyuan Tang
    • Hua Liu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Pathology, Medical School of Nantong University, Nantong, Jiangsu 226001, P.R. China
  • Pages: 973-980
    |
    Published online on: November 9, 2018
       https://doi.org/10.3892/or.2018.6857
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Abstract

Lysophospholipase1 (LYPLA1) also known as acyl‑protein thioesterase1 (APT1) belongs to the superfamily of α/β hydrolase. It has been found to have the properties of a homodimer by manifesting depalmitoylation as well as lysophospholipase activity. LYPLAs are under the control of both microRNAs, miR‑138 and miR‑424. They were observed to be significantly overexpressed in chronic lymphocytic leukemia cells. To date, LYPLAs are the sole enzymes recognized to activate depalmitoylation. In this study, we provide the expression pattern of LYPLA1 in non‑small cell lung cancer (NSCLC) using four different NSCLC cell lines. Western blot analysis and RT‑PCR were performed to detect the protein expression and mRNA expression of LYPLA1 in NSCLC cell lines. We detected the highest LYPLA1 protein expression level in SPC‑A‑1 cells followed by A549 cells, and the highest LYPLA1 mRNA expression level was detected in the SPC‑A‑1 cells followed by the H1299 cell line. We found that suppression of LYPLA1 expression using small‑interfering RNA significantly inhibited proliferation, migration and invasion of the LYPLA1‑transfected NSCLC cells. Furthermore, we explored the involvement of LYPLA1 in the regulation of epithelial‑mesenchymal transition (EMT). The epithelial marker E‑cadherin was significantly increased, while mesenchymal markers N‑cadherin, vimentin and SNAIL were markedly decreased in the LYPLA1‑silenced cells. Collectively the results of the present study suggest that the LYPLA1 gene plays a tumor‑promotor role in NSCLC cells in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Mohammed A, Zhang C, Zhang S, Shen Q, Li J, Tang Z and Liu H: Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1. Oncol Rep 41: 973-980, 2019.
APA
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., & Liu, H. (2019). Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1. Oncology Reports, 41, 973-980. https://doi.org/10.3892/or.2018.6857
MLA
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., Liu, H."Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1". Oncology Reports 41.2 (2019): 973-980.
Chicago
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., Liu, H."Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1". Oncology Reports 41, no. 2 (2019): 973-980. https://doi.org/10.3892/or.2018.6857
Copy and paste a formatted citation
x
Spandidos Publications style
Mohammed A, Zhang C, Zhang S, Shen Q, Li J, Tang Z and Liu H: Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1. Oncol Rep 41: 973-980, 2019.
APA
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., & Liu, H. (2019). Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1. Oncology Reports, 41, 973-980. https://doi.org/10.3892/or.2018.6857
MLA
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., Liu, H."Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1". Oncology Reports 41.2 (2019): 973-980.
Chicago
Mohammed, A., Zhang, C., Zhang , S., Shen, Q., Li, J., Tang, Z., Liu, H."Inhibition of cell proliferation and migration in non‑small cell lung cancer cells through the suppression of LYPLA1". Oncology Reports 41, no. 2 (2019): 973-980. https://doi.org/10.3892/or.2018.6857
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