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Article Open Access

TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway

  • Authors:
    • Jianbing Hou
    • Ailing Lv
    • Qing Deng
    • Guanghui Zhang
    • Xiaosong Hu
    • Hongjuan Cui
  • View Affiliations / Copyright

    Affiliations: Cell Biology Laboratory, State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
    Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 753-764
    |
    Published online on: November 9, 2018
       https://doi.org/10.3892/or.2018.6859
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Abstract

Trophoblast cell surface antigen 2 (TROP2), a single transmembrane domain protein, is often found to be highly expressed in various types of human cancers. However, the biological function and molecular mechanism of TROP2 in glioblastoma have not been fully elucidated, particularly in regards to cell proliferation and metastasis of glioblastoma cells. In the present study, it was demonstrated that TROP2 expression was increased in glioblastoma tissues and glioblastoma cell lines by immunohistochemical analysis and western blot analysis. High TROP2 expression was significantly correlated with the poor survival of glioblastoma patients. MTT assay, BrdU incorporation assay, flow cytometry and Transwell assay were performed to demonstrate that knockdown of TROP2 in glioblastoma cells inhibited cell proliferation and metastasis. We found that the effects of TROP2‑knockdown on glioblastoma cells were associated with the inhibition of JAK2 and STAT3 phosphorylation and decreased transcription of STAT3 target genes. In addition, blocking the activation of JAK2/STAT3 signaling by WP1066 negated the effects of TROP2 overexpression. Furthermore, exogenous IL‑6, which functions as a potent activator of JAK2/STAT3 signaling, was able to rescue the phosphorylation of JAK2 and STAT3 in TROP2‑silenced glioblastoma cells and regulate phenotypic changes in these cells. Therefore, we revealed a novel mechanism by which TROP2 activates the JAK2/STAT3 pathway to promote the growth and metastasis of glioblastoma cells. These data offer insight into the function of TROP2 in glioblastoma and indicate that TROP2 is a promising biomarker and therapeutic target for glioblastoma patients.
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Copy and paste a formatted citation
Spandidos Publications style
Hou J, Lv A, Deng Q, Zhang G, Hu X and Cui H: TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway. Oncol Rep 41: 753-764, 2019.
APA
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., & Cui, H. (2019). TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway. Oncology Reports, 41, 753-764. https://doi.org/10.3892/or.2018.6859
MLA
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., Cui, H."TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway". Oncology Reports 41.2 (2019): 753-764.
Chicago
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., Cui, H."TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway". Oncology Reports 41, no. 2 (2019): 753-764. https://doi.org/10.3892/or.2018.6859
Copy and paste a formatted citation
x
Spandidos Publications style
Hou J, Lv A, Deng Q, Zhang G, Hu X and Cui H: TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway. Oncol Rep 41: 753-764, 2019.
APA
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., & Cui, H. (2019). TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway. Oncology Reports, 41, 753-764. https://doi.org/10.3892/or.2018.6859
MLA
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., Cui, H."TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway". Oncology Reports 41.2 (2019): 753-764.
Chicago
Hou, J., Lv, A., Deng, Q., Zhang, G., Hu, X., Cui, H."TROP2 promotes the proliferation and metastasis of glioblastoma cells by activating the JAK2/STAT3 signaling pathway". Oncology Reports 41, no. 2 (2019): 753-764. https://doi.org/10.3892/or.2018.6859
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