Open Access

MicroRNA‑154 functions as a tumor suppressor in bladder cancer by directly targeting ATG7

  • Authors:
    • Junfeng Zhang
    • Shiyu Mao
    • Longsheng Wang
    • Wentao Zhang
    • Ziwei Zhang
    • Yadong Guo
    • Yuan Wu
    • Faxian Yi
    • Xudong Yao
  • View Affiliations

  • Published online on: November 21, 2018     https://doi.org/10.3892/or.2018.6879
  • Pages: 819-828
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant expression of miR‑154 is usually found in cancer studies; however, the role of miR‑154 has seldom been reported in bladder cancer (BCa). In this study, we observed that miR‑154 expression was significantly downregulated in BCa tissues and cell lines, and was associated with several clinicopathological characteristics, including advanced T stage, lymphatic invasion, and distant metastasis. Low expression level of miR‑154 was associated with poor survival outcomes in BCa patients. Overexpression of miR‑154 led to significant decrease in the proliferation, migration, and invasion of BCa cells, while knockdown of miR‑154 yielded the opposite effect. ATG7 was identified as a direct target of miR‑154. ATG7 expression was negatively correlated with miR‑154 expression in BCa tissues. Silencing of ATG7 achieved a similar effect to miR‑154 overexpression; overexpression of ATG7 reversed the inhibitory effect of miR‑154 on BCa cell proliferation, migration and invasion. A xenograft study revealed that miR‑154 inhibited BCa cell growth in vivo, and suppressed ATG7 expression. Altogether, this study demonstrated that miR‑154 may function as a tumor suppressor in BCa and indicated that miR‑154 may be a potential therapeutic target for BCa patients.
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February-2019
Volume 41 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang J, Mao S, Wang L, Zhang W, Zhang Z, Guo Y, Wu Y, Yi F and Yao X: MicroRNA‑154 functions as a tumor suppressor in bladder cancer by directly targeting ATG7. Oncol Rep 41: 819-828, 2019
APA
Zhang, J., Mao, S., Wang, L., Zhang, W., Zhang, Z., Guo, Y. ... Yao, X. (2019). MicroRNA‑154 functions as a tumor suppressor in bladder cancer by directly targeting ATG7. Oncology Reports, 41, 819-828. https://doi.org/10.3892/or.2018.6879
MLA
Zhang, J., Mao, S., Wang, L., Zhang, W., Zhang, Z., Guo, Y., Wu, Y., Yi, F., Yao, X."MicroRNA‑154 functions as a tumor suppressor in bladder cancer by directly targeting ATG7". Oncology Reports 41.2 (2019): 819-828.
Chicago
Zhang, J., Mao, S., Wang, L., Zhang, W., Zhang, Z., Guo, Y., Wu, Y., Yi, F., Yao, X."MicroRNA‑154 functions as a tumor suppressor in bladder cancer by directly targeting ATG7". Oncology Reports 41, no. 2 (2019): 819-828. https://doi.org/10.3892/or.2018.6879