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Article

TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells

  • Authors:
    • Sangmin Kim
    • Daeun You
    • Yisun Jeong
    • Jonghan Yu
    • Seok Won Kim
    • Seok Jin Nam
    • Jeong Eon Lee
  • View Affiliations / Copyright

    Affiliations: Breast Cancer Center, Samsung Medical Center, Gangnam‑gu, Seoul 06351, Republic of Korea, Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Sungkyunkwan University, Gangnam‑gu, Seoul 06351, Republic of Korea
  • Pages: 1075-1082
    |
    Published online on: December 6, 2018
       https://doi.org/10.3892/or.2018.6910
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Abstract

In a previous study, we reported that α‑smooth muscle actin (α‑SMA), one of the mesenchymal marker proteins, is highly expressed in tamoxifen‑resistant breast cancer (TamR) cells. However, the exact mechanism of α‑SMA expression in TamR cells is not fully understood. Here, we investigated the effect of TP53 on α‑SMA expression in breast cancer cells. The levels of α‑SMA mRNA and protein expression were analyzed by real‑time PCR and western blotting, respectively. In estrogen receptor‑positive [ER(+)] breast cancer patients, aberrant α‑SMA expression was found to be associated with a poor prognosis. The level of α‑SMA expression was significantly increased in established TamR cells compared to tamoxifen‑sensitive (TamS) cells. To verify the regulatory mechanism of α‑SMA expression, we analyzed diverse kinase activities between TamS and TamR cells. The activity of TP53 was markedly increased in the TamR cells. When TamS cells were treated with TP53 activator, Nutlin3 (Nut3), α‑SMA expression was increased in the TamS cells. In addition, α‑SMA expression was significantly increased by TP53 overexpression in breast cancer cells. On the contrary, the basal level of α‑SMA expression was decreased by the TP53 inhibitor, pifithrin‑α (PFT‑α). Taken together, we demonstrated that α‑SMA expression is regulated by TP53 activity in TamR cells.
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1 

Lambrechts A, Van Troys M and Ampe C: The actin cytoskeleton in normal and pathological cell motility. Int J Biochem Cell Biol. 36:1890–1909. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Jeon M, You D, Bae SY, Kim SW, Nam SJ, Kim HH, Kim S and Lee JE: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction. Oncotarget. 8:50570–50581. 2016.PubMed/NCBI

3 

Kim S, Lee J, Oh SJ, Nam SJ and Lee JE: Differential effect of EGFR inhibitors on tamoxifen-resistant breast cancer cells. Oncol Rep. 34:1613–1619. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Lee HW, Park YM, Lee SJ, Cho HJ, Kim DH, Lee JI, Kang MS, Seol HJ, Shim YM, Nam DH, et al: Alpha-smooth muscle actin (ACTA2) is required for metastatic potential of human lung adenocarcinoma. Clin Cancer Res. 19:5879–5889. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Sinn M, Denkert C, Striefler JK, Pelzer U, Stieler JM, Bahra M, Lohneis P, Dorken B, Oettle H, Riess H and Sinn BV: α-Smooth muscle actin expression and desmoplastic stromal reaction in pancreatic cancer: results from the CONKO-001 study. Br J Cancer. 111:1917–1923. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Tsujino T, Seshimo I, Yamamoto H, Ngan CY, Ezumi K, Takemasa I, Ikeda M, Sekimoto M, Matsuura N and Monden M: Stromal myofibroblasts predict disease recurrence for colorectal cancer. Clin Cancer Res. 13:2082–2090. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Aoki H, Ohnishi H, Hama K, Shinozaki S, Kita H, Osawa H, Yamamoto H, Sato K, Tamada K and Sugano K: Cyclooxygenase-2 is required for activated pancreatic stellate cells to respond to proinflammatory cytokines. Am J Physiol Cell Physiol. 292:C259–C268. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Lim MJ, Ahn J, Yi JY, Kim MH, Son AR, Lee SL, Lim DS, Kim SS, Kang MA, Han Y, et al: Induction of galectin-1 by TGF-β1 accelerates fibrosis through enhancing nuclear retention of Smad2. Exp Cell Res. 326:125–135. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Petitjean A, Mathe E, Kato S, Ishioka C, Tavtigian SV, Hainaut P and Olivier M: Impact of mutant p53 functional properties on TP53 mutation patterns and tumor phenotype: Lessons from recent developments in the IARC TP53 database. Hum Mutat. 28:622–629. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Davidoff AM, Humphrey PA, Iglehart JD and Marks JR: Genetic basis for p53 overexpression in human breast cancer. Proc Natl Acad Sci USA. 88:5006–5010. 1991. View Article : Google Scholar : PubMed/NCBI

11 

Sugrue MM, Shin DY, Lee SW and Aaronson SA: Wild-type p53 triggers a rapid senescence program in human tumor cells lacking functional p53. Proc Natl Acad Sci USA. 94:9648–9653. 1997. View Article : Google Scholar : PubMed/NCBI

12 

Royds JA and Iacopetta B: p53 and disease: When the guardian angel fails. Cell Death Differ. 13:1017–1026. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Sur S, Pagliarini R, Bunz F, Rago C, Diaz LA Jr, Kinzler KW, Vogelstein B and Papadopoulos N: A panel of isogenic human cancer cells suggests a therapeutic approach for cancers with inactivated p53. Proc Natl Acad Sci USA. 106:3964–3969. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Woods DB and Vousden KH: Regulation of p53 function. Exp Cell Res. 264:56–66. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Gasco M, Shami S and Crook T: The p53 pathway in breast cancer. Breast Cancer Res. 4:70–76. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Choi HK, Yang JW, Roh SH, Han CY and Kang KW: Induction of multidrug resistance associated protein 2 in tamoxifen-resistant breast cancer cells. Endocr Relat Cancer. 14:293–303. 2007. View Article : Google Scholar : PubMed/NCBI

17 

You D, Jung SP, Jeong Y, Bae SY, Lee JE and Kim S: Fibronectin expression is upregulated by PI-3K/Akt activation in tamoxifen-resistant breast cancer cells. BMB Rep. 50:615–620. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Gyorffy B, Lanczky A, Eklund AC, Denkert C, Budczies J, Li Q and Szallasi Z: An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat. 123:725–731. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Wang Y, Klijn JG, Zhang Y, Sieuwerts AM, Look MP, Yang F, Talantov D, Timmermans M, Meijer-van Gelder ME, et al: Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer. Lancet. 365:671–679. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Abbas T, White D, Hui L, Yoshida K, Foster DA and Bargonetti J: Inhibition of human p53 basal transcription by down-regulation of protein kinase Cdelta. J Biol Chem. 279:9970–9977. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Kim S, Han J, Kim NY, Lee SK, Cho DH, Choi MY, Kim JS, Kim JH, Choe JH, Nam SJ, et al: Effect of berberine on p53 expression by TPA in breast cancer cells. Oncol Rep. 27:210–215. 2012.PubMed/NCBI

23 

DeSantis C, Ma J, Bryan L and Jemal A: Breast cancer statistics, 2013. CA Cancer J Clin. 64:52–62. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Musgrove EA and Sutherland RL: Biological determinants of endocrine resistance in breast cancer. Nat Rev Cancer. 9:631–643. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Pohlmann PR, Mayer IA and Mernaugh R: Resistance to trastuzumab in breast cancer. Clin Cancer Res. 15:7479–7491. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Ding L, Zhang Z, Shang D, Cheng J, Yuan H, Wu Y, Song X and Jiang H: α-Smooth muscle actin-positive myofibroblasts, in association with epithelial-mesenchymal transition and lymphogenesis, is a critical prognostic parameter in patients with oral tongue squamous cell carcinoma. J Oral Pathol Med. 43:335–343. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Han J, Kim S, Yang JH, Nam SJ and Lee JE: TPA-induced p21 expression augments G2/M arrest through a p53-independent mechanism in human breast cancer cells. Oncol Rep. 27:517–522. 2012.PubMed/NCBI

28 

Chen R, Zhang Z, Xue Z, Wang L, Fu M, Lu Y, Bai L, Zhang D and Fan Z: Focal adhesion kinase (FAK) siRNA inhibits human hypertrophic scar by suppressing integrin alpha, TGF-β and α-SMA. Cell Biol Int. 38:803–808. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Li S, Butler P, Wang Y, Hu Y, Han DC, Usami S, Guan JL and Chien S: The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells. Proc Natl Acad Sci U S A. 99:3546–3551. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Wade M, Li YC and Wahl GM: MDM2, MDMX and p53 in oncogenesis and cancer therapy. Nat Rev Cancer. 13:83–96. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Enge M, Bao W, Hedstrom E, Jackson SP, Moumen A and Selivanova G: MDM2-dependent downregulation of p21 and hnRNP K provides a switch between apoptosis and growth arrest induced by pharmacologically activated p53. Cancer Cell. 15:171–183. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Levine AJ: p53, the cellular gatekeeper for growth and division. Cell. 88:323–331. 1997. View Article : Google Scholar : PubMed/NCBI

33 

Menendez D, Inga A and Resnick MA: The expanding universe of p53 targets. Nat Rev Cancer. 9:724–737. 2009. View Article : Google Scholar : PubMed/NCBI

34 

You D, Jung SP, Jeong Y, Bae SY and Kim S: Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncol Rep. 38:2551–2557. 2017. View Article : Google Scholar : PubMed/NCBI

35 

Jeong Y, You D, Kang HG, Yu J, Kim SW, Nam SJ, Lee JE and Kim S: Berberine suppresses fibronectin expression through inhibition of c-jun phosphorylation in breast cancer cells. J Breast Cancer. 21:21–27. 2018. View Article : Google Scholar : PubMed/NCBI

36 

Wu WS, Heinrichs S, Xu D, Garrison SP, Zambetti GP, Adams JM and Look AT: Slug antagonizes p53-mediated apoptosis of hematopoietic progenitors by repressing puma. Cell. 123:641–653. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim S, You D, Jeong Y, Yu J, Kim SW, Nam SJ and Lee JE: TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells. Oncol Rep 41: 1075-1082, 2019.
APA
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S.W., Nam, S.J., & Lee, J.E. (2019). TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells. Oncology Reports, 41, 1075-1082. https://doi.org/10.3892/or.2018.6910
MLA
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S. W., Nam, S. J., Lee, J. E."TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells". Oncology Reports 41.2 (2019): 1075-1082.
Chicago
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S. W., Nam, S. J., Lee, J. E."TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells". Oncology Reports 41, no. 2 (2019): 1075-1082. https://doi.org/10.3892/or.2018.6910
Copy and paste a formatted citation
x
Spandidos Publications style
Kim S, You D, Jeong Y, Yu J, Kim SW, Nam SJ and Lee JE: TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells. Oncol Rep 41: 1075-1082, 2019.
APA
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S.W., Nam, S.J., & Lee, J.E. (2019). TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells. Oncology Reports, 41, 1075-1082. https://doi.org/10.3892/or.2018.6910
MLA
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S. W., Nam, S. J., Lee, J. E."TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells". Oncology Reports 41.2 (2019): 1075-1082.
Chicago
Kim, S., You, D., Jeong, Y., Yu, J., Kim, S. W., Nam, S. J., Lee, J. E."TP53 upregulates α‑smooth muscle actin expression in tamoxifen‑resistant breast cancer cells". Oncology Reports 41, no. 2 (2019): 1075-1082. https://doi.org/10.3892/or.2018.6910
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