Downregulation of miR-95 in gastric cancer promotes EMT via regulation of Slug, thereby promoting migration and invasion

Zhang,Wei; Sun,Jing; Chen,Jinyan; Xu,Caiyun; Zhang,Lixia

doi:10.3892/or.2018.6911

Downregulation of miR-95 in gastric cancer promotes EMT via regulation of Slug, thereby promoting migration and invasion

Authors:
- Wei Zhang
- Jing Sun
- Jinyan Chen
- Caiyun Xu
- Lixia Zhang
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Affiliations: Department of Gastrointestinal Surgery, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China, Department of Emergency, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China, Department of Nuclear Medicine, Zhejiang Provincial Hospital of Traditional Chinese Medicine (The First Affiliated Hospital of Zhejiang Chinese Medical University), Hangzhou, Zhejiang 310006, P.R. China
Published online on: December 6, 2018 https://doi.org/10.3892/or.2018.6911
Pages: 1395-1403

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Abstract

miR-95 has been revealed to be aberrantly expressed in multiple types of cancer and to regulate tumor development. Moreover, miR-95 has been revealed to be downregulated in gastric cancer. However, the detailed function of miR-95 in gastric cancer has remained largely unknown. In the present study, we found that miR-95 was downregulated in 63 pairs of gastric cancer tissue samples and adjacent normal tissue samples, as well as gastric cancer cell lines. Additionally, the expression of miR-95 was associated with tumor size, tumor‑node‑metastasis (TNM) stage and lymph node metastasis. Various functional experiments, including Cell Counting Kit-8 (CCK-8), colony formation, wound healing and Transwell assays were used to explore the effect of miR-95 on cell proliferation and migration as well as invasion, respectively. Overexpression of miR-95 significantly suppressed cell proliferation, migration and invasion. Moreover, miR-95 inhibited EMT by directly binding the 3'-untranslated region (3'-UTR) of Slug. Therefore, miR-95 may be used as a novel therapeutic target for suppressing gastric cancer growth and metastasis.

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