Open Access

Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells

  • Authors:
    • Vincenzo Quagliariello
    • Mario Masarone
    • Emilia Armenia
    • Aldo Giudice
    • Manlio Barbarisi
    • Michele Caraglia
    • Alfonso Barbarisi
    • Marcello Persico
  • View Affiliations

  • Published online on: December 13, 2018     https://doi.org/10.3892/or.2018.6932
  • Pages: 1476-1486
  • Copyright: © Quagliariello et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Butyric acid (BA) has been reported to induce anticancer effects on hepatocellular carcinoma (HCC) cells both in vitro and in vivo. However, its delivery and release in cancer tissues must be optimized. On the basis of these requirements, we prepared liposomes coated with chitosan and uncoated liposomes and both types were loaded with BA through a thin-film hydration method. The liposomes coated or uncoated with chitosan had a mean hydrodynamic size of 83.5 and 110.3 nm, respectively, with a homogeneous size distribution of the particles. For evaluation of the biological effects of the nanoformulations, the hepatoblastoma (HB) HepG2 cell line was utilized. BA-loaded liposomes coated with chitosan showed a considerable higher cytotoxicity than both uncoated liposomes and free BA, with IC50 values, after 72 h of incubation, of 7.5, 2.5 and 1.6 mM, respectively. Treatment of HepG2 cells for 5 h with the BA-loaded liposomes coated with chitosan at 5 mM lowered the extent of the increase in IL-8, IL-6, TNF-α and TGF-β expression of approximately 64, 58, 85 and 73.8%, respectively, when compared to the untreated cells. The BA-loaded liposomes coated with chitosan had marked capacity to be internalized in human HB cells showing an increased cytotoxic activity when compared with free BA and important anti-inflammatory effects by inhibiting production of cytokines with a central role in liver cell survival.
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March-2019
Volume 41 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Quagliariello V, Masarone M, Armenia E, Giudice A, Barbarisi M, Caraglia M, Barbarisi A and Persico M: Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncol Rep 41: 1476-1486, 2019
APA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M. ... Persico, M. (2019). Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncology Reports, 41, 1476-1486. https://doi.org/10.3892/or.2018.6932
MLA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41.3 (2019): 1476-1486.
Chicago
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41, no. 3 (2019): 1476-1486. https://doi.org/10.3892/or.2018.6932