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Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells

  • Authors:
    • Vincenzo Quagliariello
    • Mario Masarone
    • Emilia Armenia
    • Aldo Giudice
    • Manlio Barbarisi
    • Michele Caraglia
    • Alfonso Barbarisi
    • Marcello Persico
  • View Affiliations / Copyright

    Affiliations: Division of Cardiology, Istituto Nazionale Tumori, IRCCS Fondazione G. Pascale, I-80131 Naples, Italy, Internal Medicine and Hepatology Division, Department of Medicine, University of Medicine of Salerno, Salerno, I-80123 Naples, Italy, Department of Thoracic and Cardio-Respiratory Sciences, University of Campania ‘Luigi Vanvitelli’, I-80138 Naples, Italy, Istituto Nazionale Tumori IRCCS Fondazione Pascale, I-80131 Napoli, Italy, Department of Medical, Surgical, Neurological, Metabolic and Aging Sciences, University of Campania ‘Luigi Vanvitelli’, I-80138 Naples, Italy, Department of Precision Medicine, University of Campania ‘Luigi Vanvitelli’, I-80138 Naples, Italy
    Copyright: © Quagliariello et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1476-1486
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    Published online on: December 13, 2018
       https://doi.org/10.3892/or.2018.6932
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Abstract

Butyric acid (BA) has been reported to induce anticancer effects on hepatocellular carcinoma (HCC) cells both in vitro and in vivo. However, its delivery and release in cancer tissues must be optimized. On the basis of these requirements, we prepared liposomes coated with chitosan and uncoated liposomes and both types were loaded with BA through a thin-film hydration method. The liposomes coated or uncoated with chitosan had a mean hydrodynamic size of 83.5 and 110.3 nm, respectively, with a homogeneous size distribution of the particles. For evaluation of the biological effects of the nanoformulations, the hepatoblastoma (HB) HepG2 cell line was utilized. BA-loaded liposomes coated with chitosan showed a considerable higher cytotoxicity than both uncoated liposomes and free BA, with IC50 values, after 72 h of incubation, of 7.5, 2.5 and 1.6 mM, respectively. Treatment of HepG2 cells for 5 h with the BA-loaded liposomes coated with chitosan at 5 mM lowered the extent of the increase in IL-8, IL-6, TNF-α and TGF-β expression of approximately 64, 58, 85 and 73.8%, respectively, when compared to the untreated cells. The BA-loaded liposomes coated with chitosan had marked capacity to be internalized in human HB cells showing an increased cytotoxic activity when compared with free BA and important anti-inflammatory effects by inhibiting production of cytokines with a central role in liver cell survival.
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Spandidos Publications style
Quagliariello V, Masarone M, Armenia E, Giudice A, Barbarisi M, Caraglia M, Barbarisi A and Persico M: Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncol Rep 41: 1476-1486, 2019.
APA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M. ... Persico, M. (2019). Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncology Reports, 41, 1476-1486. https://doi.org/10.3892/or.2018.6932
MLA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41.3 (2019): 1476-1486.
Chicago
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41, no. 3 (2019): 1476-1486. https://doi.org/10.3892/or.2018.6932
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Spandidos Publications style
Quagliariello V, Masarone M, Armenia E, Giudice A, Barbarisi M, Caraglia M, Barbarisi A and Persico M: Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncol Rep 41: 1476-1486, 2019.
APA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M. ... Persico, M. (2019). Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells. Oncology Reports, 41, 1476-1486. https://doi.org/10.3892/or.2018.6932
MLA
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41.3 (2019): 1476-1486.
Chicago
Quagliariello, V., Masarone, M., Armenia, E., Giudice, A., Barbarisi, M., Caraglia, M., Barbarisi, A., Persico, M."Chitosan-coated liposomes loaded with butyric acid demonstrate anticancer and anti-inflammatory activity in human hepatoma HepG2 cells". Oncology Reports 41, no. 3 (2019): 1476-1486. https://doi.org/10.3892/or.2018.6932
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