Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March-2019 Volume 41 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2019 Volume 41 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts

  • Authors:
    • Martina Niksic Zakelj
    • Ajda Prevc
    • Simona Kranjc
    • Maja Cemazar
    • Vesna Todorovic
    • Monika Savarin
    • Janez Scancar
    • Tina Kosjek
    • Blaz Groselj
    • Primoz Strojan
    • Gregor Sersa
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Oncology, Institute of Oncology Ljubljana, SI‑1000 Ljubljana, Slovenia, Department of Environmental Sciences, Jozef Stefan Institute, SI‑1000 Ljubljana, Slovenia, Department of Radiation Oncology, Institute of Oncology Ljubljana, SI‑1000 Ljubljana, Slovenia, Faculty of Medicine, University of Ljubljana, SI‑1000 Ljubljana, Slovenia
    Copyright: © Zakelj et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1658-1668
    |
    Published online on: January 9, 2019
       https://doi.org/10.3892/or.2019.6960
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Electrochemotherapy is an established local ablative method used for the treatment of different tumor types, including tumors of the head and neck area. Clinical studies have demonstrated a lower response rate of tumors that recur in pre‑irradiated area. The aim of the present study was to explore the response of experimentally induced radioresistant cells and tumors to electrochemotherapy with cisplatin or bleomycin. The radioresistant cells (FaDu‑RR) were established by fractionated irradiation of parental human squamous cell carcinoma cell line, FaDu. We compared the 2 cell lines in response to chemotherapy and electrochemotherapy with cisplatin or bleomycin in vitro and in vivo. Using specific mass spectrometry‑based analytical methods we determined the difference in the uptake of chemotherapeutics in tumors after electrochemotherapy. Additionally, we compared the capacity of the cells to repair DNA double‑strand breaks (DSB) after exposure to the drugs used in electrochemotherapy with the γH2AX foci resolution determined by immunofluorescence microscopy. Our results indicate radio‑ and cisplatin cross‑resistance, confirmed with the lower response rate of radioresistant tumors after electrochemotherapy with cisplatin. On the other hand, the sensitivity to electrochemotherapy with bleomycin was similar in both cell lines and tumors. While the uptake of chemotherapeutics after electrochemotherapy was comparable in both tumor models, there was a difference between the cell lines in capacity to repair DNA DSB‑the radioresistant cells had a lower level of DSB and faster DNA repair rate after exposure to both, cisplatin or bleomycin. Due to the higher complete response rate after electrochemotherapy with bleomycin than with cisplatin, we conclude that the results favor bleomycin‑over cisplatin‑based electrochemotherapy for treatment of radioresistant tumors and/or tumors that regrow after radiotherapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Yarmush ML, Golberg A, Serša G, Kotnik T and Miklavčič D: Electroporation-based technologies for medicine: Principles, applications and challenges. Annu Rev Biomed Eng. 16:295–320. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Mir LM, Orlowski S, Belehradek J Jr, Teissie J, Rols PM, Sersa G, Miklavcic D, Gilbert R and Heller R: Biomedical applications of electric pulses with special emphasis on antitumor electrochemotherapy. Bioelectrochem Bioenerg. 38:203–207. 1995. View Article : Google Scholar

3 

Sersa G, Bosnjak M, Cemazar M and Heller R: Preclinical studies on electrochemotherapy. Handbook of Electroporation. Miklavčič D: Springer International Publishing; Cham, Switzerland: pp. 2153–2169. 2017

4 

Calvet CY, Famin D, André FM and Mir LM: Electrochemotherapy with bleomycin induces hallmarks of immunogenic cell death in murine colon cancer cells. Oncoimmunology. 3:e281312014. View Article : Google Scholar : PubMed/NCBI

5 

Di Gennaro P, Gerlini G, Urso C, Sestini S, Brandani P, Pimpinelli N and Borgognoni L: CD4+FOXP3+ T regulatory cells decrease and CD3+CD8+ T cells recruitment in TILs from melanoma metastases after electrochemotherapy. Clin Exp Metastasis. 33:787–798. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Sersa G, Jarm T, Kotnik T, Coer A, Podkrajsek M, Sentjurc M, Miklavcic D, Kadivec M, Kranjc S, Secerov A, et al: Vascular disrupting action of electroporation and electrochemotherapy with bleomycin in murine sarcoma. Br J Cancer. 98:388–398. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Mali B, Jarm T, Snoj M, Sersa G and Miklavcic D: Antitumor effectiveness of electrochemotherapy: A systematic review and meta-analysis. Eur J Surg Oncol. 39:4–16. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Bertino G, Sersa G, De Terlizzi F, Occhini A, Plaschke CC, Groselj A, Langdon C, Grau JJ, McCaul JA, Heuveling D, et al: European research on electrochemotherapy in head and neck cancer (EURECA) project: Results of the treatment of skin cancer. Eur J Cancer. 63:41–52. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Plaschke CC, Bertino G, McCaul JA, Grau JJ, de Bree R, Sersa G, Occhini A, Groselj A, Langdon C, Heuveling DA, et al: European Research on Electrochemotherapy in Head and Neck Cancer (EURECA) project: Results from the treatment of mucosal cancers. Eur J Cancer. 87:172–181. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Spratt DE, Gordon Spratt EA, Wu S, DeRosa A, Lee NY, Lacouture ME and Barker CA: Efficacy of skin-directed therapy for cutaneous metastases from advanced cancer: A meta-analysis. J Clin Oncol. 32:3144–3155. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Mevio N, Bertino G, Occhini A, Scelsi D, Tagliabue M, Mura F and Benazzo M: Electrochemotherapy for the treatment of recurrent head and neck cancers: Preliminary results. Tumori. 98:308–313. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Groselj A, Kos B, Cemazar M, Urbancic J, Kragelj G, Bosnjak M, Veberic B, Strojan P, Miklavcic D and Sersa G: Coupling treatment planning with navigation system: A new technological approach in treatment of head and neck tumors by electrochemotherapy. Biomed Eng Online. 14 Suppl 3:S22015. View Article : Google Scholar : PubMed/NCBI

13 

Seccia V, Muscatello L, Dallan I, Bajraktari A, Briganti T, Ursino S, Galli L, Falcone A and Sellari-Franceschini S: Electrochemotherapy and its controversial results in patients with head and neck cancer. Anticancer Res. 34:967–972. 2014.PubMed/NCBI

14 

De Virgilio A, Ralli M, Longo L, Mancini P, Attanasio G, Atturo F, De Vincentiis M and Greco A: Electrochemotherapy in head and neck cancer: A review of an emerging cancer treatment (Review). Oncol Lett. 16:3415–3423. 2018.PubMed/NCBI

15 

Landström F, Ivarsson M, Von Sydow AK, Magnuson A, Von Beckerath M and Möller C: Electrochemotherapy-evidence for cell-type selectivity in vitro. Anticancer Res. 35:5813–5820. 2015.PubMed/NCBI

16 

Frandsen SK and Gehl J: A review on differences in effects on normal and malignant cells and tissues to electroporation-based therapies: A focus on calcium electroporation. Technol Cancer Res Treat. 17:1533033818788072018. View Article : Google Scholar

17 

Plaschke CC, Gothelf A, Gehl J and Wessel I: Electrochemotherapy of mucosal head and neck tumors: A systematic review. Acta Oncol. 55:1266–1272. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Mali B, Miklavcic D, Campana LG, Cemazar M, Sersa G, Snoj M and Jarm T: Tumor size and effectiveness of electrochemotherapy. Radiol Oncol. 47:32–41. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Kunte C, Letulé V, Gehl J, Dahlstroem K, Curatolo P, Rotunno P, Muir T, Occhini A, Bertino G, Powell B, et al: Electrochemotherapy in the treatment of metastatic malignant melanoma: A prospective cohort study by InspECT. Br J Dermatol. 176:1475–1485. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Campana LG, Mali B, Sersa G, Valpione S, Giorgi CA, Strojan P, Miklavcic D and Rossi CR: Electrochemotherapy in non-melanoma head and neck cancers: A retrospective analysis of the treated cases. Br J Oral Maxillofac Surg. 52:957–964. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Campana LG, Testori A, Curatolo P, Quaglino P, Mocellin S, Framarini M, Borgognoni L, Ascierto PA, Mozzillo N, Guida M, et al: Treatment efficacy with electrochemotherapy: A multi-institutional prospective observational study on 376 patients with superficial tumors. Eur J Surg Oncol. 42:1914–1923. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Barker HE, Paget JT, Khan AA and Harrington KJ: The tumour microenvironment after radiotherapy: Mechanisms of resistance and recurrence. Nat Rev Cancer. 15:409–425. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Krause M, Dubrovska A, Linge A and Baumann M: Cancer stem cells: Radioresistance, prediction of radiotherapy outcome and specific targets for combined treatments. Adv Drug Deliv Rev. 109:63–73. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Peitzsch C, Kurth I, Kunz-Schughart L, Baumann M and Dubrovska A: Discovery of the cancer stem cell related determinants of radioresistance. Radiother Oncol. 108:378–387. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Stimac M, Dolinsek T, Lampreht U, Cemazar M and Sersa G: Gene electrotransfer of plasmid with tissue specific promoter encoding shRNA against endoglin exerts antitumor efficacy against murine TS/A tumors by vascular targeted effects. PLoS One. 10:e01249132015. View Article : Google Scholar : PubMed/NCBI

26 

Dolinsek T, Prosen L, Cemazar M, Potocnik T and Sersa G: Electrochemotherapy with bleomycin is effective in BRAF mutated melanoma cells and interacts with BRAF inhibitors. Radiol Oncol. 50:274–279. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Prevc A, Bedina Zavec A, Cemazar M, Kloboves-Prevodnik V, Stimac M, Todorovic V, Strojan P and Sersa G: Bystander effect induced by electroporation is possibly mediated by microvesicles and dependent on pulse amplitude, repetition frequency and cell type. J Membr Biol. 249:703–711. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Sersa G, Miklavcic M, Cemazar M, Belehradek J Jr, Jarm T and Mir LM: Electrochemotherapy with CDDP on LPB sarcoma: Comparison of the anti-tumor effectiveness in immunocompetent and immunodefficient mice. Bioelectrochem Bioenerg. 43:279–283. 1997. View Article : Google Scholar

29 

Cemazar M, Miklavcic D and Sersa G: Intrinsic sensitivity of tumor cells to bleomycin as an indicator of tumor response to electrochemotherapy. Jpn J Cancer Res. 89:328–333. 1998. View Article : Google Scholar : PubMed/NCBI

30 

Begg A: Principles and practices of the tumor growth delay assay. Rodent Tumor Models in Experimental Cancer Therapy. Kallman RF: Pergamon Press; New York, NY: pp. 114–121. 1987

31 

Kranjc S, Kranjc M, Scancar J, Jelenc J, Sersa G and Miklavcic D: Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo. Radiol Oncol. 50:39–48. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Cemazar M, Miklavcic D, Scancar J, Dolzan V, Golouh R and Sersa G: Increased platinum accumulation in SA-1 tumour cells after in vivo electrochemotherapy with cisplatin. Br J Cancer. 79:1386–1391. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Kranjc S, Cemazar M, Sersa G, Scancar J and Grabner S: In vitro and in vivo evaluation of electrochemotherapy with trans-platinum analogue trans-[PtCl2(3-Hmpy)2]. Radiol Oncol. 51:295–306. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Martinčič A, Cemazar M, Sersa G, Kovač V, Milačič R and Ščančar J: A novel method for speciation of Pt in human serum incubated with cisplatin, oxaliplatin and carboplatin by conjoint liquid chromatography on monolithic disks with UV and ICP-MS detection. Talanta. 116:141–148. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Kosjek T, Krajnc A, Gornik T, Zigon D, Groselj A, Sersa G and Cemazar M: Identification and quantification of bleomycin in serum and tumor tissue by liquid chromatography coupled to high resolution mass spectrometry. Talanta. 160:164–171. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al: Fiji: An open source platform for biological image analysis. Nat Methods. 9:676–682. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Banáth JP, Klokov D, MacPhail SH, Banuelos CA and Olive PL: Residual gammaH2AX foci as an indication of lethal DNA lesions. BMC Cancer. 10:42010. View Article : Google Scholar : PubMed/NCBI

38 

Landström FJ, Reizenstein J, Adamsson GB, Von Beckerath M and Möller C: Long-term follow-up in patients treated with curative electrochemotherapy for cancer in the oral cavity and oropharynx. Acta Otolaryngol. 135:1070–1078. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Mcdermott N, Meunier A, Lynch TH, Hollywood D and Marignol L: Isogenic radiation resistant cell lines: Development and validation strategies. Int J Radiat Biol. 90:115–126. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Borràs-Fresneda M, Barquinero JF, Gomolka M, Hornhardt S, Rössler U, Armengol G and Barrios L: Differences in DNA repair capacity, cell death and transcriptional response after irradiation between a radiosensitive and a radioresistant cell line. Sci Rep. 6:270432016. View Article : Google Scholar : PubMed/NCBI

41 

Fukuda K, Sakakura C, Miyagawa K, Kuriu Y, Kin S, Nakase Y, Hagiwara A, Mitsufuji S, Okazaki Y, Hayashizaki Y, et al: Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation. Br J Cancer. 91:1543–1550. 2004. View Article : Google Scholar : PubMed/NCBI

42 

Gomez-Casal R, Epperly MW, Wang H, Proia DA, Greenberger JS and Gomez-Casal VL: Radioresistant human lung adenocarcinoma cells that survived multiple fractions of ionizing radiation are sensitive to HSP90 inhibition. Oncotarget. 6:44306–44322. 2015. View Article : Google Scholar : PubMed/NCBI

43 

Kurth I, Hein L, Mäbert K, Peitzsch C, Koi L, Cojoc M, Kunz-Schughart L, Baumann M and Dubrovska A: Cancer stem cell related markers of radioresistance in head and neck squamous cell carcinoma. Oncotarget. 6:34494–34509. 2015. View Article : Google Scholar : PubMed/NCBI

44 

de Llobet LI, Baro M, Figueras A, Modolell I, Da Silva MV, Muñoz P, Navarro A, Mesia R and Balart J: Development and characterization of an isogenic cell line with a radioresistant phenotype. Clin Trans Oncol. 15:189–197. 2013. View Article : Google Scholar

45 

Lynam-Lennon N, Reynolds JV, Pidgeon GP, Lysaght J, Marignol L and Maher SG: Alterations in DNA repair efficiency are involved in the radioresistance of esophageal adenocarcinoma. Radiat Res. 174:703–711. 2010. View Article : Google Scholar : PubMed/NCBI

46 

Xie L, Song X, Yu J, Wei L, Song B, Wang X and Lv L: Fractionated irradiation induced radio-resistant esophageal cancer EC109 cells seem to be more sensitive to chemotherapeutic drugs. J Exp Clin Cancer Res. 28:682009. View Article : Google Scholar : PubMed/NCBI

47 

Mihatsch J, Toulany M, Bareiss PM, Grimm S, Lengerke C, Kehlbach R and Rodemann HP: Selection of radioresistant tumor cells and presence of ALDH1 activity in vitro. Radiother Oncol. 99:300–306. 2011. View Article : Google Scholar : PubMed/NCBI

48 

Eichholtz-Wirth H, Reidel G and Hietel B: Radiation-induced transient cisplatin resistance in murine fibrosarcoma cells associated with elevated metallothionein content. Br J Cancer. 67:1001–1006. 1993. View Article : Google Scholar : PubMed/NCBI

49 

Strojan P, Haigentz M Jr, Bradford CR, Wolf GT, Hartl DM, Langendijk JA, Rinaldo A, Eisbruch A, Mendenhall WM, Forastiere AA, et al: Chemoradiotherapy vs. total laryngectomy for primary treatment of advanced laryngeal squamous cell carcinoma. Oral Oncol. 49:283–286. 2013. View Article : Google Scholar : PubMed/NCBI

50 

Hecht SM: Bleomycin: New perspectives on the mechanism of action. J Nat Prod. 63:158–168. 2000. View Article : Google Scholar : PubMed/NCBI

51 

Wang Q, Cui K, Espin-Garcia O, Cheng D, Qiu X, Chen Z, Moore M, Bristow RG, Xu W, Der S, et al: Resistance to bleomycin in cancer cell lines is characterized by prolonged doubling time, reduced DNA damage and evasion of G2/M arrest and apoptosis. PLoS One. 8:e823632013. View Article : Google Scholar : PubMed/NCBI

52 

Zuckerman JE, Raffin TA, Brown JM, Newman RA, Etiz BB and Sikic BI: In vitro selection and characterization of a bleomycin-resistant subline of B16 melanoma. Cancer Res. 46:1748–1753. 1986.PubMed/NCBI

53 

Gately DP and Howell SB: Cellular accumulation of the anticancer agent cisplatin: A review. Br J Cancer. 67:1171–1176. 1993. View Article : Google Scholar : PubMed/NCBI

54 

Kilari D, Guancial E and Kim ES: Role of copper transporters in platinum resistance. World J Clin Oncol. 7:106–113. 2016. View Article : Google Scholar : PubMed/NCBI

55 

Mesojednik S, Pavlin D, Sersa G, Coer A, Kranjc S, Grosel A, Tevz G and Cemazar M: The effect of the histological properties of tumors on transfection efficiency of electrically assisted gene delivery to solid tumors in mice. Gene Ther. 14:1261–1269. 2007. View Article : Google Scholar : PubMed/NCBI

56 

Fuertes MA, Alonso C and Pérez JM: Biochemical modulation of cisplatin mechanisms of action: Enhancement of antitumor activity and circumvention of drug resistance. Chem Rev. 103:645–662. 2003. View Article : Google Scholar : PubMed/NCBI

57 

Shen DW, Pouliot LM, Hall MD and Gottesman MM: Cisplatin resistance: A cellular self-defense mechanism resulting from multiple epigenetic and genetic changes. Pharmacol Rev. 64:706–721. 2002. View Article : Google Scholar

58 

Galluzzi L, Vitale I, Michels J, Brenner C, Szabadkai G, Harel-Bellan A, Castedo M and Kroemer G: Systems biology of cisplatin resistance: Past, present and future. Cell Death Dis. 5:e12572014. View Article : Google Scholar : PubMed/NCBI

59 

Milas L, Ito H, Hunter N, Jones S and Peters LJ: Retardation of tumor growth in mice caused by radiation-induced injury of tumor bed stroma: Dependency on tumor type. Cancer Res. 46:723–727. 1986.PubMed/NCBI

60 

Falk H, Lambaa S, Johannesen HH, Wooler G, Venzo A and Gehl J: Electrochemotherapy and calcium electroporation inducing a systemic immune response with local and distant remission of tumors in a patient with malignant melanoma-a case report. Acta Oncol. 56:1126–1131. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zakelj MN, Prevc A, Kranjc S, Cemazar M, Todorovic V, Savarin M, Scancar J, Kosjek T, Groselj B, Strojan P, Strojan P, et al: Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts. Oncol Rep 41: 1658-1668, 2019.
APA
Zakelj, M.N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M. ... Sersa, G. (2019). Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts. Oncology Reports, 41, 1658-1668. https://doi.org/10.3892/or.2019.6960
MLA
Zakelj, M. N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M., Scancar, J., Kosjek, T., Groselj, B., Strojan, P., Sersa, G."Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts". Oncology Reports 41.3 (2019): 1658-1668.
Chicago
Zakelj, M. N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M., Scancar, J., Kosjek, T., Groselj, B., Strojan, P., Sersa, G."Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts". Oncology Reports 41, no. 3 (2019): 1658-1668. https://doi.org/10.3892/or.2019.6960
Copy and paste a formatted citation
x
Spandidos Publications style
Zakelj MN, Prevc A, Kranjc S, Cemazar M, Todorovic V, Savarin M, Scancar J, Kosjek T, Groselj B, Strojan P, Strojan P, et al: Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts. Oncol Rep 41: 1658-1668, 2019.
APA
Zakelj, M.N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M. ... Sersa, G. (2019). Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts. Oncology Reports, 41, 1658-1668. https://doi.org/10.3892/or.2019.6960
MLA
Zakelj, M. N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M., Scancar, J., Kosjek, T., Groselj, B., Strojan, P., Sersa, G."Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts". Oncology Reports 41.3 (2019): 1658-1668.
Chicago
Zakelj, M. N., Prevc, A., Kranjc, S., Cemazar, M., Todorovic, V., Savarin, M., Scancar, J., Kosjek, T., Groselj, B., Strojan, P., Sersa, G."Electrochemotherapy of radioresistant head and neck squamous cell carcinoma cells and tumor xenografts". Oncology Reports 41, no. 3 (2019): 1658-1668. https://doi.org/10.3892/or.2019.6960
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team