Open Access

miR663a‑TTC22V1 axis inhibits colon cancer metastasis

  • Authors:
    • Wei Tian
    • Yantao Du
    • Yuwan Ma
    • Baozhen Zhang
    • Liankun Gu
    • Jing Zhou
    • Dajun Deng
  • View Affiliations

  • Published online on: January 16, 2019     https://doi.org/10.3892/or.2019.6969
  • Pages: 1718-1728
  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

An increasing number of studies have demonstrated that microRNAs (miRs) may act as oncogenes or anti‑oncogenes in various types of cancer, including colon cancer (CC). However, the clinical and biological significance of miR663a in the prognosis of CC and its underlying molecular mechanisms remain unknown. Using the reverse transcription‑quantitative polymerase chain reaction on CC and surgical margin tissue samples from 172 patients with CC, it was identified that miR663a was significantly downregulated in CC (P<0.001), particularly in metastatic CC (P=0.044). miR663a overexpression inhibited the proliferation and migration/invasion of CC cells in vitro, and also tumor growth and metastasis of CC cells in vivo. Additionally, miR663a target genes were analyzed. Inverse changes in tetratricopeptide repeat domain 22 variant 1 (TTC22V1) in response to alterations in miR663a expression were observed. miR663a decreased the reporter activity of the wild‑type TTC22V1‑3' untranslated region (UTR), but did not decrease that of a 3'UTR mutant. miR663a completely abolished cell migration/invasion induced by TTC22V1 containing the wild‑type 3'UTR sequence, but not that induced by TTC22V1 containing the 3'UTR mutant. An inverse correlation between miR663a and TTC22 mRNA levels was observed in CC tissues. These results suggest that TTC22V1 mRNA is a crucial miR663a target that directly promotes cell migration/invasion. TTC22, which, to the best of our knowledge, has rarely been investigated, is located in the nuclei of epithelial cells in colon stem cell niches at crypt bases, and is significantly downregulated in CC, particularly in non‑metastatic CC. High TTC22V1 expression is a significant poor survival factor for patients with CC. Collectively, the results of the present study suggested that TTC22V1 may be a metastasis‑associated gene and that the miR663a‑TTC22V1 axis inhibited CC metastasis.
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March-2019
Volume 41 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Tian W, Du Y, Ma Y, Zhang B, Gu L, Zhou J and Deng D: miR663a‑TTC22V1 axis inhibits colon cancer metastasis . Oncol Rep 41: 1718-1728, 2019
APA
Tian, W., Du, Y., Ma, Y., Zhang, B., Gu, L., Zhou, J., & Deng, D. (2019). miR663a‑TTC22V1 axis inhibits colon cancer metastasis . Oncology Reports, 41, 1718-1728. https://doi.org/10.3892/or.2019.6969
MLA
Tian, W., Du, Y., Ma, Y., Zhang, B., Gu, L., Zhou, J., Deng, D."miR663a‑TTC22V1 axis inhibits colon cancer metastasis ". Oncology Reports 41.3 (2019): 1718-1728.
Chicago
Tian, W., Du, Y., Ma, Y., Zhang, B., Gu, L., Zhou, J., Deng, D."miR663a‑TTC22V1 axis inhibits colon cancer metastasis ". Oncology Reports 41, no. 3 (2019): 1718-1728. https://doi.org/10.3892/or.2019.6969