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Article

TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway

  • Authors:
    • Wen Liu
    • Xiang Chen
    • Yanqi He
    • Yihao Tian
    • Liu Xu
    • Yanbin Ma
    • Pengchao Hu
    • Ke Su
    • Zhenyu Luo
    • Lei Wei
    • Jingwei Zhang
  • View Affiliations / Copyright

    Affiliations: Hubei Provincial Key Laboratory of Developmentally Originated Disease, Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Human Anatomy and Histology and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Renmin Hospital of Wuhan University, The First College of Clinical Medicine of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Breast and Thyroid Surgery, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei 430071, P.R. China
  • Pages: 2418-2430
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    Published online on: February 21, 2019
       https://doi.org/10.3892/or.2019.7026
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Abstract

Lung cancer is the leading cause of cancer‑associated mortality worldwide. Tumor necrosis factor α (TNF‑α) is an important cytokine in the tumor microenvironment that serves a function in the balance of cell survival and cell death pathways. Our previous studies indicated that hypoxia‑inducible factor 1α (HIF‑1α) acts downstream of TNF‑α in MCF‑7 luminal breast cancer cells. However, whether vasodilator‑stimulated phosphoprotein (VASP) is implicated in the direct regulation of HIF‑1α in response to TNF‑α in lung cancer remains unknown. In vitro studies were performed using A549 and H226 lung carcinoma cells and in vivo studies of tumor xenograft models were performed to investigate the effects of TNF‑α. The results demonstrated that TNF‑α decreased VASP expression by upregulating the expression of HIF‑1α to inhibit A549 cell proliferation and adhesion. Inhibition of transplanted tumor growth was associated with downregulation of VASP expression in nude mice. Bioinformatics analysis indicated that expression levels of VASP or HIF‑1α lead to differential outcomes of overall survival in lung carcinoma. These results suggest that the HIF‑1α/VASP signaling pathway serves an important function in the regulation of TNF‑α‑induced suppression of A549 cell proliferation and xenograft growth. This may improve our understanding of the antitumor effect of TNF‑α.
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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Chen X, He Y, Tian Y, Xu L, Ma Y, Hu P, Su K, Luo Z, Wei L, Wei L, et al: TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway. Oncol Rep 41: 2418-2430, 2019.
APA
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y. ... Zhang, J. (2019). TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway. Oncology Reports, 41, 2418-2430. https://doi.org/10.3892/or.2019.7026
MLA
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y., Hu, P., Su, K., Luo, Z., Wei, L., Zhang, J."TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway". Oncology Reports 41.4 (2019): 2418-2430.
Chicago
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y., Hu, P., Su, K., Luo, Z., Wei, L., Zhang, J."TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway". Oncology Reports 41, no. 4 (2019): 2418-2430. https://doi.org/10.3892/or.2019.7026
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Chen X, He Y, Tian Y, Xu L, Ma Y, Hu P, Su K, Luo Z, Wei L, Wei L, et al: TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway. Oncol Rep 41: 2418-2430, 2019.
APA
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y. ... Zhang, J. (2019). TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway. Oncology Reports, 41, 2418-2430. https://doi.org/10.3892/or.2019.7026
MLA
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y., Hu, P., Su, K., Luo, Z., Wei, L., Zhang, J."TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway". Oncology Reports 41.4 (2019): 2418-2430.
Chicago
Liu, W., Chen, X., He, Y., Tian, Y., Xu, L., Ma, Y., Hu, P., Su, K., Luo, Z., Wei, L., Zhang, J."TNF‑α inhibits xenograft tumor formation by A549 lung cancer cells in nude mice via the HIF‑1α/VASP signaling pathway". Oncology Reports 41, no. 4 (2019): 2418-2430. https://doi.org/10.3892/or.2019.7026
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