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Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells

  • Authors:
    • Yuxi Chen
    • Hongjun Chen
    • Hui Xie
    • Shaohui Yuan
    • Chuanbo Gao
    • Lei Yu
    • Zhenggang Bi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Teaching Experiment Center of Biotechnology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Orthopedic Surgery, The Fifth Hospital of Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2803-2817
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    Published online on: March 1, 2019
       https://doi.org/10.3892/or.2019.7040
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Abstract

Osteosarcoma (OS) is the predominant form of primary bone malignancy in children and adolescents. Although the combination of chemotherapy and modified surgical therapy leads to marked improvements in the survival rate, the therapeutic outcomes remain unsatisfactory. Therefore, the identification of novel drugs with higher efficacy and fewer side‑effects is urgently required. Proteasome inhibitors have been approved by the Food and Drug Administration (FDA) for the treatment of certain cancers, although none of them are directed against OS. Non‑covalent proteasome inhibitors, such as PI‑1840, are superior to covalent ones in numerous respects in view of their chemical structure; however, to date, no studies have been published on the effects of non‑covalent proteasome inhibitors on OS cells. In the present study, the antineoplastic effects of PI‑1840 were systematically evaluated in the OS cell lines, MG‑63 and U2‑OS. Cell viability and morphological changes were assessed by Cell Counting Kit‑8 (CCK‑8) and live/dead assays. The cell cycle was analyzed using flow cytometry (FCM) and western blot analysis (assessing the levels of the proteins p21, p27, and the tyrosine kinase, WEE1). The extent of cell apoptosis and autophagy were assessed by FCM, western blot analysis [of the apoptosis‑associated proteins, microtubule‑associated protein 1 light chain 3 α (LC3) and Beclin1], and mRFP‑GFP‑LC3 adenovirus transfection assay. Transwell and wound healing assays, and western blot analysis of the matrix metalloproteinases (MMPs)2 and 9 were performed to preliminarily evaluate the migration and invasion capability of the cells. In the present study, our results revealed that PI‑1840 inhibited the proliferation of OS cells and induced apoptosis, partly due to attenuation of the nuclear factor‑κB (NF‑κB) pathway. In addition, PI‑1840‑induced autophagy was detected, and inhibiting the autophagy of the OS cells led to an increase in the survival rate of the U2‑OS cells rather than of the MG‑63 cells. Furthermore, PI‑1840 attenuated the migration and invasion capabilities of the OS cells. In conclusion, the present study revealed PI‑1840 to be a promising drug for the treatment of OS.
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View References

1 

Bielack SS, Kempf-Bielack B, Delling G, Exner GU, Flege S, Helmke K, Kotz R, Salzer-Kuntschik M, Werner M, Winkelmann W, et al: Prognostic factors in high-grade osteosarcoma of the extremities or trunk: An analysis of 1,702 patients treated on neoadjuvant cooperative osteosarcoma study group protocols. J Clin Oncol. 20:776–790. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Harrison DJ, Geller DS, Gill JD, Lewis VO and Gorlick R: Current and future therapeutic approaches for osteosarcoma. Expert Rev Anticancer Ther. 18:39–50. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Isakoff MS, Bielack SS, Meltzer P and Gorlick R: Osteosarcoma: Current treatment and a collaborative pathway to success. J Clin Oncol. 33:3029–3035. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Smith MA, Seibel NL, Altekruse SF, Ries LA, Melbert DL, O'Leary M, Smith FO and Reaman GH: Outcomes for children and adolescents with cancer: Challenges for the twenty-first century. J Clin Oncol. 28:2625–2634. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Yamamoto N and Tsuchiya H: Chemotherapy for osteosarcoma-where does it come from? What is it? Where is it going? Expert Opin Pharmacother. 14:2183–2193. 2013.PubMed/NCBI

6 

Chen L and Madura K: Increased proteasome activity, ubiquitin-conjugating enzymes, and eEF1A translation factor detected in breast cancer tissue. Cancer Res. 65:5599–5606. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Cenci S, Oliva L, Cerruti F, Milan E, Bianchi G, Raule M, Mezghrani A, Pasqualetto E, Sitia R and Cascio P: Pivotal advance: Protein synthesis modulates responsiveness of differentiating and malignant plasma cells to proteasome inhibitors. J Leukoc Biol. 92:921–931. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Voutsadakis IA: Proteasome expression and activity in cancer and cancer stem cells. Tumor Biol. 39:10104283176922482017. View Article : Google Scholar

9 

Teicher BA and Tomaszewski JE: Proteasome inhibitors. Biochem Pharmacol. 96:1–9. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Moreau P, Masszi T, Grzasko N, Bahlis NJ, Hansson M, Pour L, Sandhu I, Ganly P, Baker BW, Jackson SR, et al: Oral ixazomib, lenalidomide, and dexamethasone for multiple myeloma. N Engl J Med. 374:1621–1634. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Palumbo A, Chanan-Khan A, Weisel K, Nooka AK, Masszi T, Beksac M, Spicka I, Hungria V, Munder M, Mateos MV, et al: Daratumumab, bortezomib, and dexamethasone for multiple myeloma. N Engl J Med. 375:754–766. 2016. View Article : Google Scholar : PubMed/NCBI

12 

McBride A, Klaus JO and Stockerl-Goldstein K: Carfilzomib: A second-generation proteasome inhibitor for the treatment of multiple myeloma. Am J Heal Pharm. 72:353–360. 2015. View Article : Google Scholar

13 

Lou Z, Ren T, Peng X, Sun Y, Jiao G, Lu Q, Zhang S, Lu X and Guo W: Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro. J Int Med Res. 41:1505–1519. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Liu R, Fu C, Sun J, Wang X, Geng S, Wang X, Zou J, Bi Z and Yang C: A new perspective for osteosarcoma therapy: Proteasome inhibition by MLN9708/2238 successfully induces apoptosis and cell cycle arrest and attenuates the invasion ability of osteosarcoma cells in vitro. Cell Physiol Biochem. 41:451–465. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Singh J, Petter RC, Baillie TA and Whitty A: The resurgence of covalent drugs. Nat Rev Drug Discov. 10:307–317. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Ozcan S, Kazi A, Marsilio F, Fang B, Guida WC, Koomen J, Lawrence HR and Sebti SM: Oxadiazole-isopropylamides as potent and noncovalent proteasome inhibitors. J Med Chem. 56:3783–3805. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Kazi A, Ozcan S, Tecleab A, Sun Y, Lawrence HR and Sebti SM: Discovery of PI-1840, a novel noncovalent and rapidly reversible proteasome inhibitor with anti-tumor activity. J Biol Chem. 289:11906–11915. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Zou J, Zhang Y, Sun J, Wang X, Tu H, Geng S, Liu R, Chen Y and Bi Z: Deoxyelephantopin induces reactive oxygen species-mediated apoptosis and autophagy in human osteosarcoma cells. Cell Physiol Biochem. 42:1812–1821. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Gu JJ, Hernandez-Ilizaliturri FJ, Kaufman GP, Czuczman NM, Mavis C, Skitzki JJ and Czuczman MS: The novel proteasome inhibitor carfilzomib induces cell cycle arrest, apoptosis and potentiates the anti-tumour activity of chemotherapy in rituximab-resistant lymphoma. Br J Haematol. 162:657–669. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Choi YH and Yoo YH: Taxol-induced growth arrest and apoptosis is associated with the upregulation of the Cdk inhibitor, p21WAF1/CIP1, in human breast cancer cells. Oncol Rep. 28:2163–2169. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Di W, Khan M, Rasul A, Sun M, Sui Y, Zhong L, Yang L, Zhu Q, Feng L and Ma T: Isoalantolactone inhibits constitutive NF-κB activation and induces reactive oxygen species-mediated apoptosis in osteosarcoma U2OS cells through mitochondrial dysfunction. Oncol Rep. 32:1585–1593. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Yu GY, Wang X, Zheng SS, Gao XM, Jia QA, Zhu WW, Lu L, Jia HL, Chen JH, Dong QZ, et al: RA190, a proteasome subunit ADRM1 inhibitor, suppresses intrahepatic cholangiocarcinoma by inducing NF-κB-mediated cell apoptosis. Cell Physiol Biochem. 47:1152–1166. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Zhang CL, Wang C, Yan WJ, Gao R, Li YH and Zhou XH: Knockdown of TNFAIP1 inhibits growth and induces apoptosis in osteosarcoma cells through inhibition of the nuclear factor-κB pathway. Oncol Rep. 32:1149–1155. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Murphy KM, Ranganathan V, Farnsworth ML, Kavallaris M and Lock RB: Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells. Cell Death Differ. 7:102–111. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Zhu Y, Li M, Wang X, Jin H, Liu S, Xu J and Chen Q: Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release. Cell Res. 22:127–141. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Djiadeu P, Farmakovski N, Azzouz D, Kotra LP, Sweezey N and Palaniyar N: Surfactant protein D regulates caspase-8-mediated cascade of the intrinsic pathway of apoptosis while promoting bleb formation. Mol Immunol. 92:190–198. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Aral K, Aral CA and Kapila Y: The role of caspase-8, caspase-9, and apoptosis inducing factor in periodontal disease. J Periodontol. 90:288–294. 2019. View Article : Google Scholar : PubMed/NCBI

28 

Chauhan D, Tian Z, Zhou B, Kuhn D, Orlowski R, Raje N, Richardson P and Anderson KC: In vitro and in vivo selective antitumor activity of a novel orally bioavailable proteasome inhibitor MLN9708 against multiple myeloma cells. Clin Cancer Res. 17:5311–5321. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Bao X, Ren T, Huang Y, Ren C, Yang K, Zhang H and Guo W: Bortezomib induces apoptosis and suppresses cell growth and metastasis by inactivation of Stat3 signaling in chondrosarcoma. Int J Oncol. 50:477–486. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Yin X, Feng C, Han L, Ma Y, Jiao Y, Wang J, Jia L, Jing F, Gao X, Zhang Y, et al: Diallyl disulfide inhibits the metastasis of type II esophageal-gastric junction adenocarcinoma cells via NF-κB and PI3K/AKT signaling pathways in vitro. Oncol Rep. 39:784–794. 2018.PubMed/NCBI

31 

Liao D, Zhong L, Duan T, Zhang RH, Wang X, Wang G, Hu K, Lv X and Kang T: Aspirin suppresses the growth and metastasis of osteosarcoma through the NF-κB pathway. Clin Cancer Res. 21:5349–5359. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Jiang Q, Pan Y, Cheng Y and Li H, Liu D and Li H: Lunasin suppresses the migration and invasion of breast cancer cells by inhibiting matrix metalloproteinase-2/-9 via the FAK/Akt/ERK and NF-κB signaling pathways. Oncol Rep. 36:253–262. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Zhang H: Targeting autophagy in lymphomas: A double-edged sword? Int J Hematol. 107:502–512. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Mehrpour M, Esclatine A, Beau I and Codogno P: Autophagy in health and disease. 1. Regulation and significance of autophagy: An overview. Am J Physiol Cell Physiol. 298:C776–C785. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Gewirtz DA: The four faces of autophagy: Implications for cancer therapy. Cancer Res. 74:647–651. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Karsli-Uzunbas G, Guo JY, Price S, Teng X, Laddha SV, Khor S, Kalaany NY, Jacks T, Chan CS, Rabinowitz JD and White E: Autophagy is required for glucose homeostasis and lung tumor maintenance. Cancer Discov. 4:914–927. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Bronietzki AW, Schuster M and Schmitz I: Autophagy in T-cell development, activation and differentiation. Immunol Cell Biol. 93:25–34. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Wu J, Gao F, Xu T, Deng X, Wang C, Yang X, Hu Z, Long Y, He X, Liang G, et al: miR-503 suppresses the proliferation and metastasis of esophageal squamous cell carcinoma by triggering autophagy via PKA/mTOR signaling. Int J Oncol. Mar 16–2018.(Epub ahead of print). View Article : Google Scholar

39 

Zhao H, Jia H, Han Q and Zhang J: Homeobox containing 1 inhibits liver cancer progression by promoting autophagy as well as inhibiting stemness and immune escape. Oncol Rep. 40:1657–1665. 2018.PubMed/NCBI

40 

White E, Mehnert JM and Chan CS: Autophagy, metabolism, and cancer. Clin Cancer Res. 21:5037–5046. 2015. View Article : Google Scholar : PubMed/NCBI

41 

Zhu J, Cai Y, Xu K, Ren X, Sun J, Lu S, Chen J and Xu P: Beclin1 overexpression suppresses tumor cell proliferation and survival via an autophagy-dependent pathway in human synovial sarcoma cells. Oncol Rep. 40:1927–1936. 2018.PubMed/NCBI

42 

Su J, Liu F, Xia M, Xu Y, Li X, Kang J, Li Y and Sun L: p62 participates in the inhibition of NF-κB signaling and apoptosis induced by sulfasalazine in human glioma U251 cells. Oncol Rep. 34:235–243. 2015. View Article : Google Scholar : PubMed/NCBI

43 

Qian HR, Shi ZQ, Zhu HP, Gu LH, Wang XF and Yang Y: Interplay between apoptosis and autophagy in colorectal cancer. Oncotarget. 8:62759–62768. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Chen H, Xie H, Yuan S, Gao C, Yu L and Bi Z: Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells. Oncol Rep 41: 2803-2817, 2019.
APA
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., & Bi, Z. (2019). Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells. Oncology Reports, 41, 2803-2817. https://doi.org/10.3892/or.2019.7040
MLA
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., Bi, Z."Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells". Oncology Reports 41.5 (2019): 2803-2817.
Chicago
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., Bi, Z."Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells". Oncology Reports 41, no. 5 (2019): 2803-2817. https://doi.org/10.3892/or.2019.7040
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Chen H, Xie H, Yuan S, Gao C, Yu L and Bi Z: Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells. Oncol Rep 41: 2803-2817, 2019.
APA
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., & Bi, Z. (2019). Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells. Oncology Reports, 41, 2803-2817. https://doi.org/10.3892/or.2019.7040
MLA
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., Bi, Z."Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells". Oncology Reports 41.5 (2019): 2803-2817.
Chicago
Chen, Y., Chen, H., Xie, H., Yuan, S., Gao, C., Yu, L., Bi, Z."Non‑covalent proteasome inhibitor PI‑1840 induces apoptosis and autophagy in osteosarcoma cells". Oncology Reports 41, no. 5 (2019): 2803-2817. https://doi.org/10.3892/or.2019.7040
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