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Article

Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death

  • Authors:
    • Yang Yang
    • Chun‑Li Yang
    • Zhen‑Jiang Zhao
    • Xiao‑Xiao Zuo
    • Tian‑Song Liang
    • Ya Yang
    • Sheng‑Lin Ma
    • Dao‑Ke Yang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China, Gynecology and Obstetrics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Emergency Department, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450053, P.R. China, Center for Translational Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 3100-3110
    |
    Published online on: March 22, 2019
       https://doi.org/10.3892/or.2019.7085
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Abstract

The pleiotropic effects of hyperthermia on cancer cells have been well documented, and microwave hyperthermia (MWHT) has been widely applied for multifarious cancer treatment. However, the mechanisms underlying the anticancer effect of MWHT combined with gemcitabine (GEM) remain poorly understood. The aim of the present study was to investigate the role of autophagy in the thermo‑chemotherapy of human squamous cell lung carcinoma cells. It was observed that MWHT combined with GEM potently suppressed the viability of NCI‑H2170 and NCI‑H1703 cells, and induced G0/G1 cell cycle arrest. Notably, MWHT with GEM induced autophagy, as indicated by the formation of autophagic vacuoles, downregulation of p62 and upregulation of light chain 3‑II. It was further demonstrated that the autophagy was due to the production of reactive oxygen species (ROS), whereas N‑acetyl cysteine, an ROS scavenger, attenuated the level of autophagy. However, when the autophagy inhibitor 3‑methyladenine was used, there was no significant change in the production of ROS. Furthermore, it was observed that MWHT combined with GEM downregulated the protein expression levels of phosphoinositide 3‑kinase (PI3K), phosphorylated (p)‑PI3K, protein kinase B (AKT), p‑AKT, mammalian target of rapamycin (mTOR), p‑mTOR, phosphorylated S6 (pS6) and p70 S6 kinase, which are associated with autophagy. In addition, the results demonstrated that ROS served as an upstream mediator of PI3K/AKT/mTOR signaling. In light of these findings, the present study provides original insights into the molecular mechanisms underlying the cell death induced by MWHT combined with GEM, and this may be a promising approach for the treatment of human squamous cell lung carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Yang CL, Zhao ZJ, Zuo XX, Liang TS, Yang Y, Ma SL and Yang DK: Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death. Oncol Rep 41: 3100-3110, 2019.
APA
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y. ... Yang, D. (2019). Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death. Oncology Reports, 41, 3100-3110. https://doi.org/10.3892/or.2019.7085
MLA
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y., Ma, S., Yang, D."Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death". Oncology Reports 41.5 (2019): 3100-3110.
Chicago
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y., Ma, S., Yang, D."Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death". Oncology Reports 41, no. 5 (2019): 3100-3110. https://doi.org/10.3892/or.2019.7085
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Yang CL, Zhao ZJ, Zuo XX, Liang TS, Yang Y, Ma SL and Yang DK: Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death. Oncol Rep 41: 3100-3110, 2019.
APA
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y. ... Yang, D. (2019). Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death. Oncology Reports, 41, 3100-3110. https://doi.org/10.3892/or.2019.7085
MLA
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y., Ma, S., Yang, D."Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death". Oncology Reports 41.5 (2019): 3100-3110.
Chicago
Yang, Y., Yang, C., Zhao, Z., Zuo, X., Liang, T., Yang, Y., Ma, S., Yang, D."Microwave hyperthermia enhances the sensitivity of lung cancer cells to gemcitabine through reactive oxygen species‑induced autophagic death". Oncology Reports 41, no. 5 (2019): 3100-3110. https://doi.org/10.3892/or.2019.7085
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