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Article

Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis

  • Authors:
    • Baochang Sun
    • Yuan Dang
    • Fan Zhang
    • Kewen Li
    • Xiaojuan Ouyang
    • Kai Wang
    • Qiaojia Huang
  • View Affiliations / Copyright

    Affiliations: The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, Fujian 350108, P.R. China, Department of Experimental Medicine, Fuzhou General Hospital, Fuzhou, Fujian 350025, P.R. China
  • Pages: 3575-3585
    |
    Published online on: April 17, 2019
       https://doi.org/10.3892/or.2019.7127
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Abstract

The aim of the present study was to investigate the expression, function and underlying molecular mechanism of the long non‑coding (lnc) RNA RP1‑163G9.1 in patients with gastric adenocarcinoma (GA). The expression levels of lncRNA RP1‑163G9.1 were determined in 112 paired clinical GA tissues by reverse transcription‑quantitative polymerase chain reaction analysis. Subsequently, the potential clinical values of lncRNA RP1‑163G9.1 were analyzed with statistical methods. Additionally, the function of lncRNA RP1‑163G9.1 was explored at the cellular level using the Cell Counting Kit‑8 proliferation assay, Transwell experiments, fluorescence in situ hybridization (FISH), colony formation assay and flow cytometry. Furthermore, the function of lncRNA RP1‑163G9.1 was assessed in vivo using subcutaneous tumorigenesis experiments in nude mice. lncRNA RP1‑163G9.1 expression in GA tissues and cells was significantly decreased when compared with that in control gastric tissues (P<0.001) or gastric epithelial cells GES‑1 (P<0.05). This finding was associated with the depth of invasion (P=0.001), lymph node metastasis (P=0.009), tumor size (P=0.037) and immunocytochemistry marker Ki‑67 (P=0.010). FISH detection demonstrated that lncRNA RP1‑163G9.1 was primarily located in the cytoplasm. Notably, overexpression of lncRNA RP1‑163G9.1 significantly decreased cell proliferation (P<0.01), colony formation (P<0.01), invasion (P<0.01) and the number of cells at the S‑phase of the cell cycle (P<0.05); However, it did not exert a significant effect on apoptosis (P>0.05). Furthermore, tumor formation experiments revealed that overexpression of lncRNA RP1‑163G9.1 inhibited cancer cell proliferation in nude mice. The present research indicated that low expression of lncRNA RP1‑163G9.1 may be associated with enhanced tumor proliferation and invasion in GA.
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1 

Wang K, Ruan J, Qian Q, Song H, Bao C, Zhang X, Kong Y, Zhang C, Hu G, Ni J and Cui D: BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer. J Nanobiotechnology. 9:232011. View Article : Google Scholar : PubMed/NCBI

2 

Wu WK, Lee CW, Cho CH, Cho CH, Fan D, Wu K, Yu J and Sung JJ: MicroRNA dysregulation in gastric cancer: A new player enters the game. Oncogene. 29:5761–5771. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Yang L: Incidence and mortality of gastric cancer in China. World J Gastroenterol. 12:17–20. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Nammi D, Srimath-Tirumala-Peddinti RC and Neelapu NR: Identification of drug targets in helicobacter pylori by in silico analysis: Possible therapeutic implications for gastric cancer. Curr Cancer Drug Targets. 16:79–98. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Sierzega M, Kaczor M, Kolodziejczyk P, Kulig J, Sanak M and Richter P: Evaluation of serum microRNA biomarkers for gastric cancer based on blood and tissue pools profiling: The importance of miR-21 and miR-331. Br J Cancer. 117:266–273. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, Tanzer A, Lagarde J, Lin W, Schlesinger F, et al: Landscape of transcription in human cells. Nature. 489:101–108. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Wiberg RA, Halligan DL, Ness RW, Necsulea A, Kaessmann H and Keightley PD: Assessing recent selection and functionality at long noncoding RNA loci in the Mouse genome. Genome Biol Evol. 7:2432–2444. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Chen X, Sun Y, Cai R, Wang G, Shu X and Pang W: Long noncoding RNA: Multiple players in gene expression. BMB Rep. 51:280–289. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Terashima M, Tange S, Ishimura A and Suzuki T: MEG3 long noncoding RNA contributes to the epigenetic regulation of epithelial-mesenchymal transition in lung cancer cell lines. J Biol Chem. 292:82–99. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Ma B, Wang J, Song Y, Gao P, Sun J, Chen X, Yang Y and Wang Z: Upregulated long intergenic noncoding RNA KRT18P55 acts as a novel biomarker for the progression of intestinal-type gastric cancer. Onco Targets Ther. 9:445–453. 2016.PubMed/NCBI

11 

Liu XH, Sun M, Nie FQ, Ge YB, Zhang EB, Yin DD, Kong R, Xia R, Lu KH, Li JH, et al: LncRNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer. 13:922014. View Article : Google Scholar : PubMed/NCBI

12 

Sun Y, Pan J, Zhang N, Wei W, Yu S and Ai L: Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-kappaB pathway. Sci Rep. 7:180792017. View Article : Google Scholar : PubMed/NCBI

13 

Zhang EB, Han L, Yin DD, Kong R, De W and Chen J: c-Myc- induced, long, noncoding H19 affects cell proliferation and predicts a poor prognosis in patients with gastric cancer. Med Oncol. 31:9142014. View Article : Google Scholar : PubMed/NCBI

14 

Zhuang M, Gao W, Xu J, Wang P and Shu Y: The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1. Biochem Biophys Res Commun. 448:315–322. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Wu S, Zheng C, Chen S, Cai X, Shi Y, Lin B and Chen Y: Overexpression of long non-coding RNA HOTAIR predicts a poor prognosis in patients with acute myeloid leukemia. Oncol Lett. 10:2410–2414. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Dang Y, Lan F, Ouyang X, Wang K, Lin Y, Yu Y, Wang L, Wang Y and Huang Q: Expression and clinical significance of long non-coding RNA HNF1A-AS1 in human gastric cancer. World J Surg Oncol. 13:3022015. View Article : Google Scholar : PubMed/NCBI

17 

Bai J, Yong HM, Chen FF, Mei PJ, Liu H, Li C, Pan ZQ, Wu YP and Zheng JN: Cullin1 is a novel marker of poor prognosis and a potential therapeutic target in human breast cancer. Ann Oncol. 24:2016–2022. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Yang B, Wei ZY, Wang BQ, Yang HC, Wang JY and Bu XY: Down-regulation of the long noncoding RNA-HOX transcript antisense intergenic RNA inhibits the occurrence and progression of glioma. J Cell Biochem. 119:2278–2287. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Tong YS, Wang XW, Zhou XL, Liu ZH, Yang TX, Shi WH, Xie HW, Lv J, Wu QQ and Cao XF: Identification of the long non-coding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma. Mol Cancer. 14:32015. View Article : Google Scholar : PubMed/NCBI

20 

Ozes AR, Wang Y, Zong X, Fang F, Pilrose J and Nephew KP: Therapeutic targeting using tumor specific peptides inhibits long non-coding RNA HOTAIR activity in ovarian and breast cancer. Sci Rep. 7:8942017. View Article : Google Scholar : PubMed/NCBI

21 

Guo JQ, Li SJ and Guo GX: Long noncoding RNA AFAP1-AS1 promotes cell proliferation and apoptosis of gastric cancer cells via PTEN/p-AKT pathway. Dig Dis Sci. 62:2004–2010. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Liu G, Xiang T, Wu QF and Wang WX: Long noncoding RNA H19-derived miR-675 enhances proliferation and invasion via RUNX1 in gastric cancer cells. Oncol Res. 23:99–107. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Lee JJ, Kim M and Kim HP: Epigenetic regulation of long noncoding RNA UCA1 by SATB1 in breast cancer. BMB Rep. 49:578–583. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Liu Z, Chen Z, Fan R, Jiang B, Chen X, Chen Q, Nie F, Lu K and Sun M: Over-expressed long noncoding RNA HOXA11-AS promotes cell cycle progression and metastasis in gastric cancer. Mol Cancer. 16:822017. View Article : Google Scholar : PubMed/NCBI

25 

Chen X and Yan GY: Novel human lncRNA-disease association inference based on lncRNA expression profiles. Bioinformatics. 29:2617–2624. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Chen X, Yan CC, Zhang X and You ZH: Long non-coding RNAs and complex diseases: From experimental results to computational models. Brief Bioinform. 18:558–576. 2017.PubMed/NCBI

27 

Zhang E, He X, Yin D, Han L, Qiu M, Xu T, Xia R, Xu L, Yin R and De W: Increased expression of long noncoding RNA TUG1 predicts a poor prognosis of gastric cancer and regulates cell proliferation by epigenetically silencing of p57. Cell Death Dis. 7:e21092016. View Article : Google Scholar : PubMed/NCBI

28 

Atanasovska B, Rensen SS, van der Sijde MR, Marsman G, Kumar V, Jonkers I, Withoff S, Shiri-Sverdlov R, Greve JWM, Faber KN, et al: A liver-specific long noncoding RNA with a role in cell viability is elevated in human nonalcoholic steatohepatitis. Hepatology. 66:794–808. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Xia T, Liao Q, Jiang X, Shao Y, Xiao B, Xi Y and Guo J: Long noncoding RNA associated-competing endogenous RNAs in gastric cancer. Sci Rep. 4:60882014. View Article : Google Scholar : PubMed/NCBI

30 

Hu Y, Wang J, Qian J, Kong X, Tang J, Wang Y, Chen H, Hong J, Zou W, Chen Y, et al: Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. Cancer Res. 74:6890–6902. 2014. View Article : Google Scholar : PubMed/NCBI

31 

He H, Wang N, Yi X, Tang C and Wang D: Long non-coding RNA H19 regulates E2F1 expression by competitively sponging endogenous miR-29a-3p in clear cell renal cell carcinoma. Cell Biosci. 7:652017. View Article : Google Scholar : PubMed/NCBI

32 

Wang H, Yu Y, Fan S and Luo L: Knockdown of long noncoding RNA TUG1 inhibits the proliferation and cellular invasion of osteosarcoma cells by sponging miR-153. Oncol Res. 26:665–673. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun B, Dang Y, Zhang F, Li K, Ouyang X, Wang K and Huang Q: Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis. Oncol Rep 41: 3575-3585, 2019.
APA
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., & Huang, Q. (2019). Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis. Oncology Reports, 41, 3575-3585. https://doi.org/10.3892/or.2019.7127
MLA
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., Huang, Q."Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis". Oncology Reports 41.6 (2019): 3575-3585.
Chicago
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., Huang, Q."Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis". Oncology Reports 41, no. 6 (2019): 3575-3585. https://doi.org/10.3892/or.2019.7127
Copy and paste a formatted citation
x
Spandidos Publications style
Sun B, Dang Y, Zhang F, Li K, Ouyang X, Wang K and Huang Q: Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis. Oncol Rep 41: 3575-3585, 2019.
APA
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., & Huang, Q. (2019). Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis. Oncology Reports, 41, 3575-3585. https://doi.org/10.3892/or.2019.7127
MLA
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., Huang, Q."Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis". Oncology Reports 41.6 (2019): 3575-3585.
Chicago
Sun, B., Dang, Y., Zhang, F., Li, K., Ouyang, X., Wang, K., Huang, Q."Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis". Oncology Reports 41, no. 6 (2019): 3575-3585. https://doi.org/10.3892/or.2019.7127
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