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Article

Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth

  • Authors:
    • Weirong Yao
    • Yingliang Li
    • Lin Zeng
    • Xiquan Zhang
    • Zhiyong Zhou
    • Meixue Zheng
    • Huiping Wan
  • View Affiliations / Copyright

    Affiliations: Oncology Department, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi 330006, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Graduate School, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 370-376
    |
    Published online on: May 21, 2019
       https://doi.org/10.3892/or.2019.7165
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Abstract

The present study aimed to investigate the antitumor effects of an intratumoral injection of dendritic cells (DCs) overexpressing interleukin-12 (IL‑12) on melanoma. DCs, isolated from mouse spleen, were gene‑modified using an IL‑12 overexpression vector. Melanoma B6 cells were injected into C57BL/6 mice to generate tumors. Thereafter, DCs overexpressing IL‑12 were injected into the tumors, and tumor volume was subsequently measured. Pathological changes in tumor tissue were detected by hematoxylin and eosin staining. The expression of interleukin-4 (IL‑4) and IL‑12 in tumors was measured by enzyme‑linked immunosorbent assay, real‑time PCR and western blotting. DCs were successfully isolated and a lentivirus vector expressing IL‑12 was constructed. After intratumoral injection of phosphate‑buffered saline (control group), tumor cells exhibited malignant growth; whereas tumors injected with DCs (DC group) or DCs + empty vector (DC + vector group) exhibited a small amount of inflammatory cell infiltration and limited areas of tissue necrosis. In contrast, tumors injected with DCs overexpressing IL‑12 (DC + IL‑12 group) displayed severe tissue necrosis, loss of cell structure, and inflammatory cell infiltration. Compared with the control group, the tumor volumes were significantly lower in the DC, the DC + vector and the DC + IL‑12 groups, while the expression of IL‑12 and IL‑4 in the tumors was significantly higher. Importantly, the most marked changes in tumor volume and IL‑12 and IL‑4 expression were in the DC + IL‑12 group, which were significantly greater than those in tumors treated with unmodified DCs. Hence, intratumoral injection of DCs overexpressing IL‑12 exerted strong antitumor effects in melanoma, and biotherapy with DCs overexpressing IL‑12 is a potential treatment strategy for melanoma.
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1 

Thomson CH, Cassell O, Peach H, Holloway S, Garioch J and Moncrieff M: Neuropathic pain and quality of life after wide local excision and sentinel lymph node biopsy for melanoma: A multicentre study. Melanoma Res. 27:121–125. 2017. View Article : Google Scholar : PubMed/NCBI

2 

de Vries IJ, Lesterhuis WJ, Barentsz JO, Verdijk P, van Krieken JH, Boerman OC, Oyen WJ, Bonenkamp JJ, Boezeman JB, Adema GJ, et al: Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy. Nat Biotechnol. 23:1407–1413. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Cheng Y, Ma XL, Wei YQ and Wei XW: Potential roles and targeted therapy of the CXCLs/CXCR2 axis in cancer and inflammatory diseases. Biochim Biophys Acta Rev Cancer. 1871:289–312. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Barry RJ, Tallouzi MO, Bucknall N, Mathers JM, Murray PI, Calvert MJ, Moore DJ and Denniston AK: Anti-tumour necrosis factor biological therapies for the treatment of uveitic macular oedema (UMO) for non-infectious uveitis. Cochrane Database Syst Rev. 12:CD0125772018.PubMed/NCBI

5 

Mandalà M and Rutkowski P: Rational combination of cancer immunotherapy in melanoma. Virchows Arch. 474:433–447. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Summerfield A and McCullough KC: Dendritic cells in innate and adaptive immune responses against influenza virus. Viruses. 1:1022–1034. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Mellman I: Dendritic cells: Master regulators of the immune response. Cancer Immunol Res. 1:145–149. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Liu J, Cao S, Kim S, Chung EY, Homma Y, Guan X, Jimenez V and Ma X: Interleukin-12: An update on its immunological activities, signaling and regulation of gene expression. Curr Immunol Rev. 1:119–137. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Lasek W, Zagożdżon R and Jakobisiak M: Interleukin-12: Still a promising candidate for tumor immunotherapy? Cancer Immunol Immunother. 63:419–435. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Tagawa T, Albanese M, Bouvet M, Moosmann A, Mautner J, Heissmeyer V, Zielinski C, Lutter D, Hoser J, Hastreiter M, et al: Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 213:2065–2080. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Cemazar M, Ambrozic Avgustin J, Pavlin D, Sersa G, Poli A, Krhac Levacic A, Tesic N, Lampreht Tratar U, Rak M and Tozon N: Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours. Vet Comp Oncol. 15:641–654. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Komai-Koma M, Wang E, Kurowska-Stolarska M, Li D, McSharry C and Xu D: Interleukin-33 promoting Th1 lymphocyte differentiation dependents on IL-12. Immunobiol. 221:412–417. 2016. View Article : Google Scholar

13 

Lu X: Impact of IL-12 in cancer. Curr Cancer Drug Target. 17:682–697. 2017. View Article : Google Scholar

14 

Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y and Wu C: Mycobacterium tuberculosis-Specific IL-21+IFN-γ+CD4+ T cells are regulated by IL-12. PLoS One. 11:e01473562016. View Article : Google Scholar : PubMed/NCBI

15 

Feng C, Feng M, Jiao R, Liu D, Jin Y, Zhao X and Xiao R: Effect of Dezocine on IL-12 and IL-10 secretion and lymphocyte activation by culturing dendritic cells from human umbilical cord blood. Europ J Pharmacol. 796:110–114. 2017. View Article : Google Scholar

16 

Thada S, Ponnana M, Sivangala R, Joshi L, Alasandagutti M, Ansari MS, Schumann RR, Valluri V and Gaddam S: Polymorphisms of IFN-γ (+874A/T) and IL-12 (+1188A/C) in tuberculosis patients and their household contacts in Hyderabad, India. Hum Immunol. 77:559–565. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Naito S, von Eschenbach AC, Giavazzi R and Fidler IJ: Growth and metastasis of tumor cells isolated from a human renal cell carcinoma implanted into different organs of nude mice. Cancer Res. 46:4109–4115. 1986.PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Ribas A, Hamid O, Daud A, Hodi FS, Wolchok JD, Kefford R, Joshua AM, Patnaik A, Hwu WJ, Weber JS, et al: Association of pembrolizumab with tumor response and survival among patients with advanced melanoma. JAMA. 315:1600–1609. 2016. View Article : Google Scholar : PubMed/NCBI

20 

See P, Dutertre CA, Chen J, Günther P, McGovern N, Irac SE, Gunawan M, Beyer M, Händler K, Duan K, et al: Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 356:eaag30092017. View Article : Google Scholar : PubMed/NCBI

21 

Bhattacharya P, Thiruppathi M, Elshabrawy HA, Alharshawi K, Kumar P and Prabhakar BS: GM-CSF: An immune modulatory cytokine that can suppress autoimmunity. Cytokine. 75:261–271. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Deng J, Li Y, Pennati A, Yuan S, Wu JH, Waller EK and Galipeau J: GM-CSF and IL-4 fusion cytokine induces B cell-dependent hematopoietic regeneration. Mol Ther. 25:416–426. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Lutz MB, Suri RM, Niimi M, Ogilvie AL, Kukutsch NA, Rössner S, Schuler G and Austyn JM: Immature dendritic cells generated with low doses of GM-CSF in the absence of IL-4 are maturation resistant and prolong allograft survival in vivo. Eur J Immunol. 30:1813–1822. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Fukao T, Tanabe M, Terauchi Y, Ota T, Matsuda S, Asano T, Kadowaki T, Takeuchi T and Koyasu S: PI3K-mediated negative feedback regulation of IL-12 production in DCs. Nat Immunol. 3:875–881. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Kamensek U, Cemazar M, Lampreht Tratar U, Ursic K and Sersa G: Antitumor in situ vaccination effect of TNFα and IL-12 plasmid DNA electrotransfer in a murine melanoma model. Cancer Immunol Immunother. 67:785–795. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Oliveira Brito PK, Goncalves TE, Fernandes FF, Miguel CB, Rodrigues WF, Lazo Chica JE, Roque-Barreira MC and da Silva TA: Systemic effects in naïve mice injected with immunomodulatory lectin ArtinM. PLoS One. 12:e01871512017. View Article : Google Scholar : PubMed/NCBI

27 

Sonabend AM, Velicu S, Ulasov IV, Han Y, Tyler B, Brem H, Matar MM, Fewell JG, Anwer K and Lesniak MS: A safety and efficacy study of local delivery of interleukin-12 transgene by PPC polymer in a model of experimental glioma. Anticancer Drugs. 19:133–142. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Kobiyama K, Temizoz B, Kanuma T, Ozasa K, Momota M, Yamamoto T, Aoshi T, Kuroda E and Ishii KJ: Species-dependent role of type I IFNs and IL-12 in the CTL response induced by humanized CpG complexed with β-glucan. Eur J Immunol. 46:1142–1151. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Yoshimoto T, Yasuda K, Tanaka H, Nakahira M, Imai Y, Fujimori Y and Nakanishi K: Basophils contribute to T(H)2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells. Nat Immunol. 10:706–712. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Yeap WH, Wong KL, Shimasaki N, Teo EC, Quek JK, Yong HX, Diong CP, Bertoletti A, Linn YC and Wong SC: CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes. Sci Rep. 6:343102016. View Article : Google Scholar : PubMed/NCBI

31 

Helmy A, Guilfoyle MR, Carpenter KLH, Pickard JD, Menon DK and Hutchinson PJ: Recombinant human interleukin-1 receptor antagonist promotes M1 microglia biased cytokines and chemokines following human traumatic brain injury. J Cereb Blood Flow Metab. 36:1434–1448. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Lee HL, Park MH, Song JK, Jung YY, Kim Y, Kim KB, Hwang DY, Yoon do Y, Song MJ, Han SB and Hong JT: Tumor growth suppressive effect of IL-4 through p21-mediated activation of STAT6 in IL-4Rα overexpressed melanoma models. Oncotarget. 7:23425–23438. 2016.PubMed/NCBI

33 

Sznol M: Betting on immunotherapy for melanoma. Curr Oncol Rep. 11:397–404. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao W, Li Y, Zeng L, Zhang X, Zhou Z, Zheng M and Wan H: Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth. Oncol Rep 42: 370-376, 2019.
APA
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., & Wan, H. (2019). Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth. Oncology Reports, 42, 370-376. https://doi.org/10.3892/or.2019.7165
MLA
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., Wan, H."Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth". Oncology Reports 42.1 (2019): 370-376.
Chicago
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., Wan, H."Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth". Oncology Reports 42, no. 1 (2019): 370-376. https://doi.org/10.3892/or.2019.7165
Copy and paste a formatted citation
x
Spandidos Publications style
Yao W, Li Y, Zeng L, Zhang X, Zhou Z, Zheng M and Wan H: Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth. Oncol Rep 42: 370-376, 2019.
APA
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., & Wan, H. (2019). Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth. Oncology Reports, 42, 370-376. https://doi.org/10.3892/or.2019.7165
MLA
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., Wan, H."Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth". Oncology Reports 42.1 (2019): 370-376.
Chicago
Yao, W., Li, Y., Zeng, L., Zhang, X., Zhou, Z., Zheng, M., Wan, H."Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth". Oncology Reports 42, no. 1 (2019): 370-376. https://doi.org/10.3892/or.2019.7165
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