Efficient primary culture model of patient‑derived tumor cells from colorectal cancer using a Rho‑associated protein kinase inhibitor and feeder cells

  • Authors:
    • Hye Kyung Hong
    • Dae Hee Pyo
    • Tae Won Kim
    • Nak Hyeon Yun
    • Yeo Song Lee
    • Su Jeong Song
    • Woo Yong Lee
    • Yong Beom Cho
  • View Affiliations

  • Published online on: August 20, 2019     https://doi.org/10.3892/or.2019.7285
  • Pages: 2029-2038
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In vitro culture of patient‑derived tumor cells offers many advantages in the development of novel therapies for colorectal cancer. Although various culture systems have been developed, the long‑term expansion of patient‑derived tumor cells remains challenging. The present results suggested that tumor cells isolated from colorectal cancer patient‑derived xenografts can be efficiently immortalized in conditioned medium from irradiated feeder cells containing Y‑27632, a rho‑associated coiled‑coil containing protein kinase (ROCK) inhibitor. Patient‑derived tumor cells proliferated rapidly, reaching 90‑95% confluence in ~6 days. Short tandem repeat analysis suggested that these tumor tissues and cultured cells presented 13 identical short tandem repeat loci, including Amelogenin, Penta E, Penta D, D2S1338 and D19S433. Their epithelial phenotype was confirmed by staining for epithelial cell adhesion molecule and cytokeratin 20, whereas vimentin was used as a mesenchymal marker. When cells were transferred to 3D cultures, they continued to proliferate, forming well‑defined tumor spheroids. Expression levels of human telomerase reverse transcriptase and C‑Myc mRNA were increased in cultured cells. Finally, immortalized cells were used for the screening of 65 anticancer drugs approved by the Food and Drug Administration, allowing the identification of gene‑drug associations. In the present study, primary culture models of colorectal cancer were efficiently established using a ROCK inhibitor and feeder cells, and this approach could be used for personalized treatment strategies for patients with colorectal cancer.
View Figures
View References

Related Articles

Journal Cover

November-2019
Volume 42 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hong HK, Pyo DH, Kim TW, Yun NH, Lee YS, Song SJ, Lee WY and Cho YB: Efficient primary culture model of patient‑derived tumor cells from colorectal cancer using a Rho‑associated protein kinase inhibitor and feeder cells. Oncol Rep 42: 2029-2038, 2019
APA
Hong, H.K., Pyo, D.H., Kim, T.W., Yun, N.H., Lee, Y.S., Song, S.J. ... Cho, Y.B. (2019). Efficient primary culture model of patient‑derived tumor cells from colorectal cancer using a Rho‑associated protein kinase inhibitor and feeder cells. Oncology Reports, 42, 2029-2038. https://doi.org/10.3892/or.2019.7285
MLA
Hong, H. K., Pyo, D. H., Kim, T. W., Yun, N. H., Lee, Y. S., Song, S. J., Lee, W. Y., Cho, Y. B."Efficient primary culture model of patient‑derived tumor cells from colorectal cancer using a Rho‑associated protein kinase inhibitor and feeder cells". Oncology Reports 42.5 (2019): 2029-2038.
Chicago
Hong, H. K., Pyo, D. H., Kim, T. W., Yun, N. H., Lee, Y. S., Song, S. J., Lee, W. Y., Cho, Y. B."Efficient primary culture model of patient‑derived tumor cells from colorectal cancer using a Rho‑associated protein kinase inhibitor and feeder cells". Oncology Reports 42, no. 5 (2019): 2029-2038. https://doi.org/10.3892/or.2019.7285