Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
November-2019 Volume 42 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2019 Volume 42 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion

  • Authors:
    • Xiao Han
    • Xiaoxue Li
    • Huan Liu
    • Hongshuo Zhang
    • Aoying Li
    • Ming Dong
    • Yuefei Xu
    • Bin Yan
    • Linlin Sui
    • Ying Kong
  • View Affiliations / Copyright

    Affiliations: Core Laboratory Glycobiol and Glycogen, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Clinical Medicine of 2016, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Gynecology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116044, P.R. China
  • Pages: 2065-2074
    |
    Published online on: September 16, 2019
       https://doi.org/10.3892/or.2019.7317
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

O‑linked β‑N‑acetylglucosamine (O‑GlcNAc) modification is a dynamic post‑translational modification process that is involved in many crucial biological processes, including cell cycle regulation, nutrient metabolism and extracellular signaling. This dynamic modification is dependent on the ambient glucose concentration and is catalyzed and removed by O‑GlcNAc transferase (OGT) and O‑GlcNAcase (OGA), respectively. The present study aimed to determine the role of O‑GlcNAcylation during embryo implantation by inhibiting or enhancing its function and expression. The results revealed that the expression of O‑GlcNAc‑modified proteins in the human secretory endometrium was higher than that of the endometrium during the proliferative phase, as determined via western blotting and immunohistochemistry. Additionally, the level of endometrial O‑GlcNAc modification increased gradually from the pre‑receptive to the receptive phase, which was then decreased during the non‑receptive phase. In endometrial cells, RNA interference was utilized to reduce the expression of two key O‑GlcNAc synthesis and decomposition enzymes, OGT and OGA, to indirectly increase or decrease levels of O‑GlcNAc modification. The results revealed that increasing the level of O‑GlcNAc modification enhanced cellular proliferation, migration, invasion and adhesion, thereby promoting embryo implantation. It is hypothesized that O‑GlcNAc modification serves an important role in the regulation of endometrial receptivity and embryo implantation. The results of the present study may have important implications for the understanding of female fertility and may help improve infertility treatments.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Torres CR and Hart GW: Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes. Evidence for O-linked GlcNAc. J Biol Chem. 259:3308–3317. 1984.PubMed/NCBI

2 

Holt GD and Hart GW: The subcellular distribution of terminal N-acetylglucosamine moieties. Localization of a novel protein-saccharide linkage, O-linked GlcNAc. J Biol Chem. 261:8049–8057. 1986.PubMed/NCBI

3 

Paria BC, Huet-Hudson YM and Dey SK: Blastocyst's state of activity determines the ‘window’ of implantation in the receptive mouse uterus. Proc Natl Acad Sci USA. 90:10159–10162. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Yoshinaga K: Uterine receptivity for blastocyst implantation. Ann N Y Acad Sci. 541:424–431. 1988. View Article : Google Scholar : PubMed/NCBI

5 

Psychoyos A: Hormonal control of ovoimplantation. Vitam Horm. 31:201–256. 1973. View Article : Google Scholar : PubMed/NCBI

6 

Lim HJ and Dey SK: HB-EGF: A unique mediator of embryo-uterine interactions during implantation. Exp Cell Res. 315:619–626. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Gu J, Fan J, Xu Y, Xie Y, Gong T and Kong Y: Regulatory function of β1, 4-galac tosyltransferase I expression on Lewis-Y glycan and embryo implantation. Gene. 562:220–225. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Gu J, Sui LL, Cui D, Ma YN, Zhu CY and Kong Y: Effects of LeY glycan expression on embryo implantation. Eur Rev Med Pharmacol Sci. 20:3327–3335. 2016.PubMed/NCBI

9 

Slawson C, Pidala J and Potter R: Increased N-acetyl-beta- glucosaminidase activity in primary breast carcinomas corresponds to a decrease in N-acetylglucosamine containing proteins. Biochim Biophys Acta. 1537:147–157. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Murray MJ and Lessey BA: Embryo implantation and tumor metastasis: Common pathways of invasion and angiogenesis. Semin Reprod Endocrinol. 17:275–290. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Hannan NJ, Paiva P, Dimitriadis E and Salamonsen LA: Models for study of human embryo implantation: Choice of cell lines? Biol Reprod. 82:235–245. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2018. View Article : Google Scholar

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Ma XH, Hu SJ, Yu H, Xu LB and Yang ZM: Differential expression of transcriptional repressor snail gene at implantation site in mouse uterus. Mol Reprod Dev. 73:133–141. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Dey SK, Lim H, Das SK, Reese J, Paria BC, Daikoku T and Wang H: Molecular cues to implantation. Endocr Rev. 25:341–373. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Baldin V, Lukas J, Marcote MJ, Pagano M and Draetta G: Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 7:812–821. 1993. View Article : Google Scholar : PubMed/NCBI

17 

Yin XM: Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10:161–167. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Dennis JW, Nabi IR and Demetriou M: Metabolism, cell surface organization, and disease. Cell. 139:1229–1241. 2009. View Article : Google Scholar : PubMed/NCBI

19 

de Queiroz RM, Carvalho E and Dias WB: O-GlcNAcylation: The sweet side of the cancer. Front Oncol. 4:1322014. View Article : Google Scholar : PubMed/NCBI

20 

Marshall S, Bacote V and Traxinger RR: Discovery of a metabolic pathway mediating glucose-induced desensitization of the glucose transport system. Role of hexosamine biosynthesis in the induction of insulin resistance. J Biol Chem. 266:4706–4712. 1991.PubMed/NCBI

21 

Wells L, Vosseller K and Hart GW: A role for N-acetylglucosamine as a nutrient sensor and mediator of insulin resistance. Cell Mol Life Sci. 60:222–228. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Lee TI and Young RA: Transcriptional regulation and its misregulation in disease. Cell. 152:1237–1251. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Koo CY, Muir KW and Lam EW: FOXM1: From cancer initiation to progression and treatment. Biochim Biophys Acta. 1819:28–37. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Khan Z and Bisen PS: Oncoapoptotic signaling and deregulated target genes in cancers: Special reference to oral cancer. Biochim Biophys Acta. 1836:123–145. 2013.PubMed/NCBI

25 

Krzeslak A, Pomorski L and Lipinska A: Elevation of nucleocytoplasmic beta-N-acetylglucosaminidase (O-GlcNAcase) activity in thyroid cancers. Int J Mol Med. 25:643–648. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Gu Y, Mi W, Ge Y, Liu H, Fan Q, Han C, Yang J, Han F, Lu X and Yu W: GlcNAcylation plays an essential role in breast cancer metastasis. Cancer Res. 70:6344–6351. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Mi W, Gu Y, Han C, Liu H, Fan Q, Zhang X, Cong Q and Yu W: O-GlcNAcylation is a novel regulator of lung and colon cancer malignancy. Biochim Biophys Acta. 1812:514–519. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Shi Y, Tomic J, Wen F, Shaha S, Bahlo A, Harrison R, Dennis JW, Williams R, Gross BJ, Walker S, et al: Aberrant O-GlcNAcylation characterizes chronic lymphocytic leukemia. Leukemia. 24:1588–1598. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Shafi R, Iyer SP, Ellies LG, O'Donnell N, Marek KW, Chui D, Hart GW and Marth JD: The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci USA. 97:5735–5739. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Jang H, Kim TW, Yoon S, Choi SY, Kang TW, Kim SY, Kwon YW, Cho EJ and Youn HD: O-GlcNAc regulates pluripotency and reprogramming by directly acting on core components of the pluripotency network. Cell Stem Cell. 11:62–74. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Lefebvre T, Pinte S, Guérardel C, Deltour S, Martin-Soudant N, Slomianny MC, Michalski JC and Leprince D: The tumor suppressor HIC1 (hypermethylated in cancer 1) is O-GlcNAc glycosylated. Eur J Biochem. 271:3843–3854. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Tai HC, Khidekel N, Ficarro SB, Peters EC and Hsieh-Wilson LC: Parallel identification of O-GlcNAc-modified proteins from cell lysates. J Am Chem Soc. 126:10500–10501. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Schlummer S, Vetter R, Kuder N, Henkel A, Chen YX, Li YM, Kuhlmann J and Waldmann H: Influence of serine O-glycosylation or O-phosphorylation close to the vJun nuclear localisation sequence on nuclear import. Chembiochem. 7:88–97. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Teng CB, Diao HL, Ma XH, Xu LB and Yang ZM: Differential expression and activation of Stat3 during mouse embryo implantation and decidualization. Mol Reprod Dev. 69:1–10. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Cameron AM, Lawless SJ and Pearce EJ: Metabolism and acetylation in innate immune cell function and fate. Semin Immunol. 28:408–416. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Hart GW, Housley MP and Slawson C: Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins. Nature. 446:1017–1022. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Han X, Li X, Liu H, Zhang H, Li A, Dong M, Xu Y, Yan B, Sui L, Kong Y, Kong Y, et al: O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion. Oncol Rep 42: 2065-2074, 2019.
APA
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M. ... Kong, Y. (2019). O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion. Oncology Reports, 42, 2065-2074. https://doi.org/10.3892/or.2019.7317
MLA
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M., Xu, Y., Yan, B., Sui, L., Kong, Y."O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion". Oncology Reports 42.5 (2019): 2065-2074.
Chicago
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M., Xu, Y., Yan, B., Sui, L., Kong, Y."O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion". Oncology Reports 42, no. 5 (2019): 2065-2074. https://doi.org/10.3892/or.2019.7317
Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Li X, Liu H, Zhang H, Li A, Dong M, Xu Y, Yan B, Sui L, Kong Y, Kong Y, et al: O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion. Oncol Rep 42: 2065-2074, 2019.
APA
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M. ... Kong, Y. (2019). O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion. Oncology Reports, 42, 2065-2074. https://doi.org/10.3892/or.2019.7317
MLA
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M., Xu, Y., Yan, B., Sui, L., Kong, Y."O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion". Oncology Reports 42.5 (2019): 2065-2074.
Chicago
Han, X., Li, X., Liu, H., Zhang, H., Li, A., Dong, M., Xu, Y., Yan, B., Sui, L., Kong, Y."O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion". Oncology Reports 42, no. 5 (2019): 2065-2074. https://doi.org/10.3892/or.2019.7317
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team