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Article

The oncogenic role of NKAP in the growth and invasion of colon cancer cells

  • Authors:
    • Weibin Shu
    • Guoqin Liu
    • Yunpeng Dai
    • Alei Feng
    • Zheng Chen
    • Junqing Han
    • Xinyu Li
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of General Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 2130-2138
    |
    Published online on: September 18, 2019
       https://doi.org/10.3892/or.2019.7322
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Abstract

It has been reported that NF‑κB activating protein (NKAP) is a transcriptional repressor of the Notch signaling pathway and is involved in the proliferation and survival of hematopoietic stem cells. In the present study, we aimed to investigate the effect of NKAP on the progression and metastasis of colon cancer. The results of immunohistochemical staining and western blot analysis showed that NKAP was upregulated in colon cancer tissues, and its expression was associated with colon cancer stages. CCK‑8, colony formation, Transwell, and flow cytometry assays were used to demonstrate that NKAP knockdown significantly suppressed the proliferation and invasion of HCT116 and HT‑29 cells, and also induced apoptosis and autophagy. By contrast, NKAP overexpression markedly promoted the proliferation and invasion of HCT‑15 cells, and inhibited cell apoptosis and autophagy. Moreover, we observed that NKAP knockdown inhibited the epithelial‑mesenchymal transition (EMT) process in HCT116 and HT‑29 cells, while NKAP overexpression promoted EMT in HCT‑15 cells. Furthermore, NKAP knockdown inhibited activation of the Akt/mTOR signaling pathway by downregulating the phosphorylation of Akt and mTOR, as well as their downstream proteins, whereas NKAP overexpression promoted the Akt/mTOR signaling pathway. Additionally, expression of P65 was downregulated by silencing of NKAP and upregulated by NKAP overexpression. These data suggest that NKAP functions as an oncogene in the growth and invasion of colon cancer in vitro.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A and Jemal A: Colorectal cancer statistics, 2017. CA Cancer J Clin. 67:177–193. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Vatandoust S, Price TJ and Karapetis CS: Colorectal cancer: Metastases to a single organ. World J. Gastroenterol. 21:11767–11776. 2015.

4 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Chen D, Li Z, Yang Q, Zhang J, Zhai Z and Shu HB: Identification of a nuclear protein that promotes NF-kappaB activation. Biochem Biophys Res Commun. 310:720–724. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Pajerowski AG, Nguyen C, Aghajanian H, Shapiro MJ and Shapiro VS: NKAP is a transcriptional repressor of notch signaling and is required for T cell development. Immunity. 30:696–707. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Thapa P, Das J, Mcwilliams D, Shapiro M, Sundsbak R, Nelson-Holte M, Tangen S, Anderson J, Desiderio S, Hiebert S, et al: The transcriptional repressor NKAP is required for the development of iNKT cells. Nat Commun. 4:15822013. View Article : Google Scholar : PubMed/NCBI

8 

Hsu FC, Pajerowski AG, Nelson-Holte M, Sundsbak R and Shapiro VS: NKAP is required for T cell maturation and acquisition of functional competency. J Exp Med. 208:1291–1304. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Pajerowski AG, Shapiro MJ, Gwin K, Sundsbak R, Nelson-Holte M, Medina K and Shapiro VS: Adult hematopoietic stem cells require NKAP for maintenance and survival. Blood. 116:2684–2693. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Thapa P, Chen MW, Mcwilliams DC, Belmonte P, Constans M, Sant'Angelo DB and Shapiro VS: NKAP regulates invariant NKT cell proliferation and differentiation into ROR-γt-expressing NKT17 cells. J Immunol. 196:4987–4998. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Burgute BD, Peche VS, Steckelberg AL, Glöckner G, Gaßen B, Gehring NH and Noegel AA: NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins. Nucleic Acids Res. 42:3177–3193. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Li T, Chen L, Cheng J, Dai J, Huang Y, Zhang J, Liu Z, Li A, Li N, Wang H, et al: SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores. Nat Commun. 7:129692016. View Article : Google Scholar : PubMed/NCBI

13 

Wu QB and Sun GP: Expression of COX-2 and HER-2 in colorectal cancer and their correlation. World J Gastroenterol. 21:6206–6214. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Cotter TG: Apoptosis and cancer: The genesis of a research field. Nat Rev Cancer. 9:501–507. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Tang X, Zha L, Li H, Liao G, Huang Z, Peng X and Wang Z: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway. Oncol Rep. 38:2023–2032. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Liu J, Wang H, Yin Y, Li Q and Zhang M: NKAP functions as an oncogene and its expression is induced by CoCl2 treatment in breast cancer via AKT/mTOR signaling pathway. Cancer Manag Res. 10:5091–5100. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Oguma K, Oshima H, Aoki M, Uchio R, Naka K, Nakamura S, Hirao A, Saya H, Taketo MM and Oshima M: Activated macrophages promote Wnt signalling through tumour necrosis factor-alpha in gastric tumour cells. EMBO J. 27:1671–1681. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Amado NG, Predes D, Moreno MM, Carvalho IO, Mendes FA and Abreu JG: Flavonoids and Wnt/β-catenin signaling: Potential role in colorectal cancer therapies. Int J Mol Sci. 15:12094–12106. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Saito T, Masuda N, Miyazaki T, Kanoh K, Suzuki H, Shimura T, Asao T and Kuwano H: Expression of EphA2 and E-cadherin in colorectal cancer: Correlation with cancer metastasis. Oncol Rep. 11:605–611. 2004.PubMed/NCBI

21 

Murray L: The role of E-cadherin in colon cancer drug resistanceUniv Glasgow; 2010

22 

Kwak JM, Min BW, Lee JH, Choi JS, Lee SI, Park SS, Kim J, Um JW, Kim SH and Moon HY: The prognostic significance of E-cadherin and liver intestine-cadherin expression in colorectal cancer. Dis Colon Rectum. 50:1873–1880. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Croce CM and Reed JC: Finally, an apoptosis-targeting therapeutic for cancer. Cancer Res. 76:5914–5920. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Fesik SW: Promoting apoptosis as a strategy for cancer drug discovery. Nat Rev Cancer. 5:876–885. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Huang Q, Li S, Cheng P, Deng M, He X, Wang Z, Yang CH, Zhao XY and Huang J: High expression of anti-apoptotic protein Bcl-2 is a good prognostic factor in colorectal cancer: Result of a meta-analysis. World J Gastroenterol. 23:5018–5033. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Parrish AB, Freel CD and Kornbluth S: Cellular mechanisms controlling caspase activation and function. Cold Spring Harb Perspect Biol. 5:2013. View Article : Google Scholar : PubMed/NCBI

27 

Igney FH and Krammer PH: Death and anti-death: Tumour resistance to apoptosis. Nat Rev Cancer. 2:277–288. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Gui D, Guo Y, Wang F, Liu W, Chen J, Chen Y, Huang J and Wang N: Astragaloside IV, a novel antioxidant, prevents glucose-induced podocyte apoptosis in vitro and in vivo. PLoS One. 7:e398242012. View Article : Google Scholar : PubMed/NCBI

29 

Martinou JC and Youle RJ: Mitochondria in apoptosis: Bcl-2 family members and mitochondrial dynamics. Dev Cell. 21:92–101. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Wu R, Tang S, Wang M, Xu X, Yao C and Wang S: MicroRNA-497 induces apoptosis and suppresses proliferation via the Bcl-2/Bax-caspase9-caspase3 pathway and cyclin D2 protein in HUVECs. PLoS One. 11:e01670522016. View Article : Google Scholar : PubMed/NCBI

31 

He C and Klionsky DJ: Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet. 43:67–93. 2009. View Article : Google Scholar : PubMed/NCBI

32 

White E: Deconvoluting the context-dependent role for autophagy in cancer. Nat Rev Cancer. 12:401–410. 2012. View Article : Google Scholar : PubMed/NCBI

33 

White E: The role for autophagy in cancer. J Clin Invest. 125:42–46. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Burman C and Ktistakis NT: Autophagosome formation in mammalian cells. Semin Immunopathol. 32:397–413. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Tan S, Shi H, Ba M, Lin S, Tang H, Zeng X and Zhang X: miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy. Int J Mol Med. 37:1030–1038. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Jiang QG, Li TY, Liu DN and Zhang HT: PI3K/Akt pathway involving into apoptosis and invasion in human colon cancer cells LoVo. Mol Biol Rep. 41:3359–3367. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Malinowsky K, Nitsche U, Janssen KP, Bader FG, Späth C, Drecoll E, Keller G, Höfler H, Slotta-Huspenina J and Becker KF: Activation of the PI3K/AKT pathway correlates with prognosis in stage II colon cancer. Br J Cancer. 110:2081–2089. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Alao JP: The regulation of Cyclin D1 degradation: Roles in cancer development and the potential for therapeutic invention. Mol Cancer. 6:242007. View Article : Google Scholar : PubMed/NCBI

39 

Jiang BH: PI3K/AKT and mTOR/p70S6K1 signaling pathways in human cancer. Curr Cancer Drug Targets. 13:2332013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shu W, Liu G, Dai Y, Feng A, Chen Z, Han J and Li X: The oncogenic role of NKAP in the growth and invasion of colon cancer cells. Oncol Rep 42: 2130-2138, 2019.
APA
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., & Li, X. (2019). The oncogenic role of NKAP in the growth and invasion of colon cancer cells. Oncology Reports, 42, 2130-2138. https://doi.org/10.3892/or.2019.7322
MLA
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., Li, X."The oncogenic role of NKAP in the growth and invasion of colon cancer cells". Oncology Reports 42.5 (2019): 2130-2138.
Chicago
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., Li, X."The oncogenic role of NKAP in the growth and invasion of colon cancer cells". Oncology Reports 42, no. 5 (2019): 2130-2138. https://doi.org/10.3892/or.2019.7322
Copy and paste a formatted citation
x
Spandidos Publications style
Shu W, Liu G, Dai Y, Feng A, Chen Z, Han J and Li X: The oncogenic role of NKAP in the growth and invasion of colon cancer cells. Oncol Rep 42: 2130-2138, 2019.
APA
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., & Li, X. (2019). The oncogenic role of NKAP in the growth and invasion of colon cancer cells. Oncology Reports, 42, 2130-2138. https://doi.org/10.3892/or.2019.7322
MLA
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., Li, X."The oncogenic role of NKAP in the growth and invasion of colon cancer cells". Oncology Reports 42.5 (2019): 2130-2138.
Chicago
Shu, W., Liu, G., Dai, Y., Feng, A., Chen, Z., Han, J., Li, X."The oncogenic role of NKAP in the growth and invasion of colon cancer cells". Oncology Reports 42, no. 5 (2019): 2130-2138. https://doi.org/10.3892/or.2019.7322
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