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Article

Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells

  • Authors:
    • Bin Zhao
    • Caiyan Gao
    • Daixiu Shi
    • Jie Mao
    • Jun Zhao
    • Lingyun Guo
    • Jiwu Guo
    • Zuoyi Jiao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, P.R. China
  • Pages: 2670-2679
    |
    Published online on: September 20, 2019
       https://doi.org/10.3892/or.2019.7327
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Abstract

As an important regulator of neddylation, neural precursor cell expressed developmentally downregulated 8 (Nedd8)‑conjugating enzyme E2M (UBE2M) mediates cullin neddylation. Upregulation of the neddylation pathway is associated with tumor progression in intrahepatic cholangiocarcinoma (ICC). The present study was designed to assess the effects of Nedd8‑conjugating enzyme UBE2M knockdown on intrahepatic cholangiocarcinoma cells, and to determine the potential underlying mechanisms. UBE2M and associated protein expression levels were determined via immunohistochemistry and western blotting. ICC cells were transfected with short hairpin RNA to knockdown UBE2M expression. Cell Counting Kit‑8 and colony formation assays, and xenograft experiments were used to examine cell viability and colony survival in vitro, and tumor formation in vivo. Survival was evaluated using Kaplan‑Meier analysis and log‑rank tests. Patients with ICC presenting high expression of UBE2M exhibited worse accumulative recurrence and overall survival compared with patients with low expression. Knockdown of UBE2M expression led to a decrease in the viability and clonogenic survival of QBC939 and HUCCT1 cells, and suppressed tumor formation in vivo. UBE2M silencing caused accumulation of cullin‑RING ligase substrates (chromatin‑licensing and DNA replication factor 1 and origin recognition complex subunit 1), inducing DNA damage responses and apoptosis. The present findings suggested that UBE2M serves an important role in ICC progression and may present as a novel target for the treatment of ICC.
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1 

Bedford L, Lowe J, Dick LR, Mayer RJ and Brownell JE: Ubiquitin-like protein conjugation and the ubiquitin-proteasome system as drug targets. Nat Rev Drug Discov. 10:29–46. 2011. View Article : Google Scholar : PubMed/NCBI

2 

O'Connor HF and Huibregtse JM: Enzyme-substrate relationships in the ubiquitin system: Approaches for identifying substrates of ubiquitin ligases. Cell Mol Life Sci. 74:3363–3375. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Petroski MD and Deshaies RJ: Function and regulation of cullin-RING ubiquitin ligases. Nat Rev Mol Cell Biol. 6:9–20. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Deshaies RJ, Emberley ED and Saha A: Control of cullin-ring ubiquitin ligase activity by nedd8. Subcell Biochem. 54:41–56. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Ying J, Zhang M, Qiu X and Lu Y: Targeting the neddylation pathway in cells as a potential therapeutic approach for diseases. Cancer Chemother Pharmacol. 81:797–808. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Xirodimas DP: Novel substrates and functions for the ubiquitin-like molecule NEDD8. Biochem Soc Trans. 36:802–806. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zuo W, Huang F, Chiang YJ, Li M, Du J, Ding Y, Zhang T, Lee HW, Jeong LS, Chen Y, et al: C-Cbl-mediated neddylation antagonizes ubiquitination and degradation of the TGF-β type II receptor. Mol Cell. 49:499–510. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Zhao Y, Morgan MA and Sun Y: Targeting neddylation pathways to inactivate cullin-RING ligases for anticancer therapy. Antioxid Redox Signal. 21:2383–2400. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Watson IR, Irwin MS and Ohh M: NEDD8 pathways in cancer, sine quibus non. Cancer Cell. 19:168–176. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF and Schulman BA: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 33:483–495. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Cukras S, Morffy N, Ohn T and Kee Y: Inactivating UBE2M impacts the DNA damage response and genome integrity involving multiple cullin ligases. PLoS One. 9:e1018442014. View Article : Google Scholar : PubMed/NCBI

12 

Xu B, Deng Y, Bi R, Guo H, Shu C, Shah NK, Chang J, Liu G, Du Y, Wei W and Wang C: A first-in-class inhibitor, MLN4924 (pevonedistat), induces cell-cycle arrest, senescence, and apoptosis in human renal cell carcinoma by suppressing UBE2M-dependent neddylation modification. Cancer Chemother Pharmacol. 81:1083–1093. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Zhou W, Xu J, Tan M, Li H, Li H, Wei W and Sun Y: UBE2M is a stress-inducible dual E2 for neddylation and ubiquitylation that promotes targeted degradation of UBE2F. Mol Cell. 70:1008–1024. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Gao Q, Yu GY, Shi JY, Li LH, Zhang WJ, Wang ZC, Yang LX, Duan M, Zhao H, Wang XY, et al: Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target. Oncotarget. 5:7820–7832. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Altekruse SF, Devesa SS, Dickie LA, McGlynn KA and Kleiner DE: Histological classification of liver and intrahepatic bile duct cancers in SEER registries. J Registry Manag. 38:201–205. 2011.PubMed/NCBI

16 

Bertuccio P, Bosetti C, Levi F, Decarli A, Negri E and La Vecchia C: A comparison of trends in mortality from primary liver cancer and intrahepatic cholangiocarcinoma in Europe. Ann Oncol. 24:1667–1674. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Bridgewater J, Galle PR, Khan SA, Llovet JM, Park JW, Patel T, Pawlik TM and Gores GJ: Guidelines for the diagnosis and management of intrahepatic cholangiocarcinoma. J Hepatol. 60:1268–1289. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Poultsides GA, Zhu AX, Choti MA and Pawlik TM: Intrahepatic cholangiocarcinoma. Surg Clin North Am. 90:817–837. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Andersen JB, Spee B, Blechacz BR, Avital I, Komuta M, Barbour A, Conner EA, Gillen MC, Roskams T, Roberts LR, et al: Genomic and genetic characterization of cholangiocarcinoma identifies therapeutic targets for tyrosine kinase inhibitors. Gastroenterology. 14:1021–1031. 2012. View Article : Google Scholar

21 

Barrett T, Wilhite SE, Ledoux P, Evangelista C, Kim IF, Tomashevsky M, Marshall KA, Phillippy KH, Sherman PM, Holko M, et al: NCBI GEO: Archive for functional genomics data sets-update. Nucleic Acids Res. 41:D991–D995. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Szklarczyk D, Gable AL, Lyon D, Junge A, Wyder S, Huerta-Cepas J, Simonovic M, Doncheva NT, Morris JH, Bork P, et al: STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res. 47:D607–D613. 2019. View Article : Google Scholar : PubMed/NCBI

23 

Li L, Kang J, Zhang W, Cai L, Wang S, Liang Y, Jiang Y, Liu X, Zhang Y, Ruan H, et al: Validation of NEDD8-conjugating enzyme UBC12 as a new therapeutic target in lung cancer. EBioMedicine. 45:81–91. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Brown JS, Lukashchuk N, Sczaniecka-Clift M, Britton S, le Sage C, Calsou P, Calsou P, Beli P, Galanty Y and Jackson SP: Neddylation promotes ubiquitylation and release of ku from DNA-damage sites. Cell Rep. 11:704–714. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Jimeno S, Fernandez-Avila MJ, Cruz-Garcia A, Cepeda-Garcia C, Gomez-Cabello D and Huertas P: Neddylation inhibits CtIP-mediated resection and regulates DNA double strand break repair pathway choice. Nucleic Acids Res. 43:987–999. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Mavros MN, Economopoulos KP, Alexiou VG and Pawlik TM: Treatment and prognosis for patients with intrahepatic cholangiocarcinoma: Systematic review and meta-analysis. JAMA Surg. 149:565–574. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Li L, Wang M, Yu G, Chen P, Li H, Wei D, Zhu J, Xie L, Jia H, Shi J, et al: Overactivated neddylation pathway as a therapeutic target in lung cancer. J Natl Cancer Inst. 106:dju0832014. View Article : Google Scholar : PubMed/NCBI

28 

Wang W, Liu Z, Qu P, Zhou Z, Zeng Y, Fan J, Liu Y, Guo Y and Qiu J: Knockdown of regulator of cullins-1 (ROC1) expression induces bladder cancer cell cycle arrest at the G2 phase and senescence. PLoS One. 8:e627342013. View Article : Google Scholar : PubMed/NCBI

29 

Lan H, Tang Z, Jin H and Sun Y: Neddylation inhibitor MLN4924 suppresses growth and migration of human gastric cancer cells. Sci Rep. 6:242182016. View Article : Google Scholar : PubMed/NCBI

30 

Wen M, Kwon Y, Wang Y, Mao JH and Wei G: Elevated expression of UBE2T exhibits oncogenic properties in human prostate cancer. Oncotarget. 6:25226–25239. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Ueki T, Park JH, Nishidate T, Kijima K, Hirata K, Nakamura Y and Katagiri T: Ubiquitination and downregulation of BRCA1 by ubiquitin-conjugating enzyme E2T overexpression in human breast cancer cells. Cancer Res. 69:8752–8760. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Wu S and Yu L: Targeting cullin-RING ligases for cancer treatment: Rationales, advances and therapeutic implications. Cytotechnology. 68:1–8. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Jia L, Soengas MS and Sun Y: ROC1/RBX1 E3 ubiquitin ligase silencing suppresses tumor cell growth via sequential induction of G2-M arrest, apoptosis, and senescence. Cancer Res. 69:4974–4982. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Zhou L, Zhang W, Sun Y and Jia L: Protein neddylation and its alterations in human cancers for targeted therapy. Cell Signal. 44:92–102. 2018. View Article : Google Scholar : PubMed/NCBI

35 

Zhang W, Liang Y, Li L, Wang X, Yan Z, Dong C, Zeng MS, Zhong Q, Liu XK, Yu J, et al: The nedd8-activating enzyme inhibitor MLN4924 (TAK-924/Pevonedistat) induces apoptosis via c-myc-noxa axis in head and neck squamous cell carcinoma. Cell Prolif. 52:e125362019. View Article : Google Scholar : PubMed/NCBI

36 

Seifi-Alan M, Shams R, Bandehpour M, Mirfakhraie R and Ghafouri-Fard S: Neuropilin-1 expression is associated with lymph node metastasis in breast cancer tissues. Cancer Manag Res. 10:1969–1974. 2018. View Article : Google Scholar : PubMed/NCBI

37 

Hosoya N and Miyagawa K: Targeting DNA damage response in cancer therapy. Cancer Sci. 105:370–388. 2018. View Article : Google Scholar

38 

Affar EB, Germain M, Winstall E, Vodenicharov M, Shah RG, Salvesen GS and Poirier GG: Caspase-3-mediated processing of poly(ADP-ribose) glycohydrolase during apoptosis. J Biol Chem. 276:2935–2942. 2001. View Article : Google Scholar : PubMed/NCBI

39 

Pistritto G, Trisciuoglio D, Ceci C, Garufi A and D'Orazi G: Apoptosis as anticancer mechanism: Function and dysfunction of its modulators and targeted therapeutic strategies. Aging (Albany NY). 8:603–619. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Chin L, Artandi SE, Shen Q, Tam A, Lee SL, Gottlieb GJ, Greider CW and DePinho RA: P53 deficiency rescues the adverse effects of telomere loss and cooperates with telomere dysfunction to accelerate carcinogenesis. Cell. 97:527–538. 1999. View Article : Google Scholar : PubMed/NCBI

41 

Titen SW and Golic KG: Telomere loss provokes multiple pathways to apoptosis and produces genomic instability in Drosophila melanogaster. Genetics. 180:1821–1832. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Kee Y, Huang M, Chang S, Moreau LA, Park E, Smith PG and D'Andrea AD: Inhibition of the nedd8 system sensitizes cells to DNA interstrand cross-linking agents. Mol Cancer Res. 10:369–377. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao B, Gao C, Shi D, Mao J, Zhao J, Guo L, Guo J and Jiao Z: Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells. Oncol Rep 42: 2670-2679, 2019.
APA
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L. ... Jiao, Z. (2019). Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells. Oncology Reports, 42, 2670-2679. https://doi.org/10.3892/or.2019.7327
MLA
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L., Guo, J., Jiao, Z."Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells". Oncology Reports 42.6 (2019): 2670-2679.
Chicago
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L., Guo, J., Jiao, Z."Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells". Oncology Reports 42, no. 6 (2019): 2670-2679. https://doi.org/10.3892/or.2019.7327
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao B, Gao C, Shi D, Mao J, Zhao J, Guo L, Guo J and Jiao Z: Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells. Oncol Rep 42: 2670-2679, 2019.
APA
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L. ... Jiao, Z. (2019). Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells. Oncology Reports, 42, 2670-2679. https://doi.org/10.3892/or.2019.7327
MLA
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L., Guo, J., Jiao, Z."Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells". Oncology Reports 42.6 (2019): 2670-2679.
Chicago
Zhao, B., Gao, C., Shi, D., Mao, J., Zhao, J., Guo, L., Guo, J., Jiao, Z."Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells". Oncology Reports 42, no. 6 (2019): 2670-2679. https://doi.org/10.3892/or.2019.7327
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