Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells

Hou,Xiaoyang; Pang,Yanyu; Li,Xinxin; Yang,Chunsheng; Liu,Wenlou; Jiang,Guan; Liu,Yanqun

doi:10.3892/or.2019.7329

Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells

Authors:
- Xiaoyang Hou
- Yanyu Pang
- Xinxin Li
- Chunsheng Yang
- Wenlou Liu
- Guan Jiang
- Yanqun Liu
View Affiliations

Affiliations: Department of Dermatology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Dermatology, The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China
Published online on: September 20, 2019 https://doi.org/10.3892/or.2019.7329
Pages: 2512-2520
Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

A novel core‑shell type thermo‑nanoparticle (CSTNP) co‑loaded with temozolomide (TMZ) and the fluorescein new indocyanine green dye IR820 (termed IT‑CSTNPs) was designed and combined with a near‑infrared (NIR) laser to realize its photothermal conversion. The IT‑CSTNPs were prepared using a two‑step synthesis method and comprised a thermosensitive shell and a biodegradable core. IR820 and TMZ were entrapped in the shell and the core, respectively. Dynamic light scattering results demonstrated that the average hydrodynamic size of the IT‑CSTNPs was 196.4±3.1 nm with a ζ potential of ‑24.9±1.3 mV. The encapsulation efficiencies of TMZ and IR820 were 6.1 and 16.6%, respectively. Temperature increase curves under NIR laser irradiation indicated that the IT‑CSTNPs exhibited the desired photothermal conversion efﬁciency. The in vitro drug release curves revealed a suitable release capability of IT‑CSTNP under physiological conditions, whereas NIR laser irradiation accelerated the drug release. Inverted fluorescence microscopy and flow cytometry results revealed that the uptake of IT‑CSTNPs by A375 melanoma cells occurred in a concentration‑dependent manner. Confocal laser scanning microscopy results indicated that IT‑CSTNPs entered tumour cells via endocytosis and were located in intercellular lysosomes. In summary, the present study explored the photothermal conversion capability, cellular uptake, and intracellular localization of IT‑CSTNPs.

View Figures

View References

1	Damsky WE and Bosenberg M: Melanocytic nevi and melanoma: Unraveling a complex relationship. Oncogene. 36:5771–5792. 2017. View Article : Google Scholar : PubMed/NCBI
2	Tripp MK, Watson M, Balk SJ, Swetter SM and Gershenwald JE: State of the science on prevention and screening to reduce melanoma incidence and mortality: The time is now. CA Cancer J Clin. 66:460–480. 2016. View Article : Google Scholar : PubMed/NCBI
3	Chiarion-Sileni V, Guida M, Ridolfi L, Romanini A, Del Bianco P, Pigozzo J, Brugnara S, Colucci G, Ridolfi R and De Salvo GL; Italian Melanoma Intergroup (IMI), : Central nervous system failure in melanoma patients: Results of a randomised, multicentre phase 3 study of temozolomide- and dacarbazine-based regimens. Br J Cancer. 104:1816–1821. 2011. View Article : Google Scholar : PubMed/NCBI
4	Kim C, Lee CW, Kovacic L, Shah A, Klasa R and Savage KJ: Long-term survival in patients with metastatic melanoma treated with DTIC or temozolomide. Oncologist. 15:765–771. 2010. View Article : Google Scholar : PubMed/NCBI
5	Jahangirian H, Kalantari K, Izadiyan Z, Rafiee-Moghaddam R, Shameli K and Webster TJ: A review of small molecules and drug delivery applications using gold and iron nanoparticles. Int J Nanomedicine. 14:1633–1657. 2019. View Article : Google Scholar : PubMed/NCBI
6	Jindal AB: The effect of particle shape on cellular interaction and drug delivery applications of micro- and nanoparticles. Int J Pharm. 532:450–465. 2017. View Article : Google Scholar : PubMed/NCBI
7	Hoshyar N, Gray S, Han H and Bao G: The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction. Nanomedicine (Lond). 11:673–692. 2016. View Article : Google Scholar : PubMed/NCBI
8	Samanta D, Meiser JL and Zare RN: Polypyrrole nanoparticles for tunable, pH-sensitive and sustained drug release. Nanoscale. 7:9497–9504. 2015. View Article : Google Scholar : PubMed/NCBI
9	Sheng W, He S, Seare WJ and Almutairi A: Review of the progress toward achieving heat confinement-the holy grail of photothermal therapy. J Biom Opt. 22:809012017. View Article : Google Scholar
10	Zhang X, Du J, Guo Z, Yu J, Gao Q, Yin W, Zhu S, Gu Z and Zhao Y: Efficient near infrared light triggered nitric oxide release nanocomposites for sensitizing mild photothermal therapy. Adv Sci (Weinh). 6:18011222018. View Article : Google Scholar : PubMed/NCBI
11	D'Acunto M: Detection of intracellular gold nanoparticles: An overview. Materials (Basel). 11:E8822018. View Article : Google Scholar : PubMed/NCBI
12	Doughty ACV, Hoover AR, Layton E, Murray CK, Howard EW and Chen WR: Nanomaterial applications in photothermal therapy for cancer. Materials (Basel). 12:E7792019. View Article : Google Scholar : PubMed/NCBI
13	Kim H, Chung K, Lee S, Kim DH and Lee H: Near-infrared light-responsive nanomaterials for cancer theranostics. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 8:23–45. 2016. View Article : Google Scholar : PubMed/NCBI
14	Zhou B, Li Y, Niu G, Lan M, Jia Q and Liang Q: Near-infrared organic dye-based nanoagent for the photothermal therapy of cancer. ACS Appl Mater Interfaces. 8:29899–29905. 2016. View Article : Google Scholar : PubMed/NCBI
15	Chen Y, Chen Q, Zhu Q, Liu J, Li Y, Gao X, Chen D and Zhu X: Small-molecular theranostic assemblies functionalized by doxorubicin-hyaluronic acid-methotrexate prodrug for multiple tumor targeting and imaging-guided combined chemo-photothermal therapy. Mol Pharm. 16:2470–2480. 2019. View Article : Google Scholar : PubMed/NCBI
16	Wu B, Wan B, Lu ST, Deng K, Li XQ, Wu BL, Li YS, Liao RF, Huang SW and Xu HB: Near-infrared light-triggered theranostics for tumor-specific enhanced multimodal imaging and photothermal therapy. Int J Nanomed. 12:4467–4478. 2017. View Article : Google Scholar
17	Hu X, Tian H, Jiang W, Song A, Li Z and Luan Y: Rational design of IR820- and ce6-based versatile micelle for single NIR laser-induced imaging and dual-modal Phototherapy. Small. 14:e18029942018. View Article : Google Scholar : PubMed/NCBI
18	Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, Felix R and Riess H: The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol. 43:33–56. 2002. View Article : Google Scholar : PubMed/NCBI
19	Li W, Peng J, Tan L, Wu J, Shi K, Qu Y, Wei X and Qian Z: Mild photothermal therapy/photodynamic therapy/chemotherapy of breast cancer by Lyp-1 modified Docetaxel/IR820 Co-loaded micelles. Biomaterials. 106:119–133. 2016. View Article : Google Scholar : PubMed/NCBI
20	Mukherjee A, Waters AK, Kalyan P, Achrol AS, Kesari S and Yenugonda VM: Lipid-polymer hybrid nanoparticles as a next-generation drug delivery platform: State of the art, emerging technologies, and perspectives. Int J Nanomedicine. 14:1937–1952. 2019. View Article : Google Scholar : PubMed/NCBI
21	Mandal B, Bhattacharjee H, Mittal N, Sah H, Balabathula P, Thoma LA and Wood GC: Core-shell-type lipid-polymer hybrid nanoparticles as a drug delivery platform. Nanomedicine. 9:474–491. 2013. View Article : Google Scholar : PubMed/NCBI
22	de Matos MBC, Beztsinna N, Heyder C, Fens MHAM, Mastrobattista E, Schiffelers RM, Leneweit G and Kok RJ: Thermosensitive liposomes for triggered release of cytotoxic proteins. Eur J Pharm Biopharm. 132:211–221. 2018. View Article : Google Scholar : PubMed/NCBI
23	Turner DC, Moshkelani D, Shemesh CS, Luc D and Zhang H: Near-infrared image-guided delivery and controlled release using optimized thermosensitive liposomes. Pharm Res. 29:2092–2103. 2012. View Article : Google Scholar : PubMed/NCBI
24	Tong L, Liao Q, Zhao Y, Huang H, Gao A, Zhang W, Gao X, Wei W, Guan M, Chu PK and Wang H: Near-infrared light control of bone regeneration with biodegradable photothermal osteoimplant. Biomaterials. 193:1–11. 2019. View Article : Google Scholar : PubMed/NCBI
25	Albert C, Huang N, Tsapis N, Geiger S, Rosilio V, Mekhloufi G, Chapron D, Robin B, Beladjine M, Nicolas V, et al: Bare and sterically stabilized PLGA nanoparticles for the stabilization of pickering emulsions. Langmuir. 34:13935–13945. 2018. View Article : Google Scholar : PubMed/NCBI
26	Li B, Xu H, Li Z, Yao M, Xie M, Shen H, Shen S, Wang X and Jin Y: Bypassing multidrug resistance in human breast cancer cells with lipid/polymer particle assemblies. Int J Nanomedicine. 7:187–197. 2012.PubMed/NCBI
27	Chen J, Shao R, Zhang XD and Chen C: Applications of nanotechnology for melanoma treatment, diagnosis, and theranostics. Int J Nanomedicine. 8:2677–2688. 2013. View Article : Google Scholar : PubMed/NCBI
28	Silva IP and Long GV: Systemic therapy in advanced melanoma: Integrating targeted therapy and immunotherapy into clinical practice. Curr Opin Oncol. 29:484–492. 2017. View Article : Google Scholar : PubMed/NCBI
29	Yingchoncharoen P, Kalinowski DS and Richardson DR: Lipid-Based drug delivery systems in cancer therapy: What is available and what is yet to come. Pharmacol Rev. 68:701–787. 2016. View Article : Google Scholar : PubMed/NCBI
30	Sah H, Thoma LA, Desu HR, Sah E and Wood GC: Concepts and practices used to develop functional PLGA-based nanoparticulate systems. Int J Nanomedicine. 8:747–765. 2013. View Article : Google Scholar : PubMed/NCBI
31	Ananta JS, Paulmurugan R and Massoud TF: Temozolomide- loaded PLGA nanoparticles to treat glioblastoma cells: A biophysical and cell culture evaluation. Neurol Res. 38:51–59. 2016. View Article : Google Scholar : PubMed/NCBI
32	Cheow WS and Hadinoto K: Factors affecting drug encapsulation and stability of lipid-polymer hybrid nanoparticles. Colloids Surf B Biointerfaces. 85:214–220. 2011. View Article : Google Scholar : PubMed/NCBI
33	Chen WR, Singhal AK, Liu H and Nordquist RE: Antitumor immunity induced by laser immunotherapy and its adoptive transfer. Cancer Res. 61:459–461. 2001.PubMed/NCBI
34	Hu Y, Chi C, Wang S, Wang L, Liang P, Liu F, Shang W, Wang W, Zhang F, Li S, et al: A comparative study of clinical intervention and interventional photothermal therapy for pancreatic cancer. Adv Mater. 29:2017. View Article : Google Scholar
35	Hunyadi A, Csabi J, Martins A, Molnar J, Balazs A and Toth G: Backstabbing P-gp: Side-chain cleaved ecdysteroid 2,3-dioxolanes hyper-sensitize MDR cancer cells to doxorubicin without efflux inhibition. 22:E1992017.PubMed/NCBI
36	Chen HH, Lu IL, Liu TI, Tsai YC, Chiang WH, Lin SC and Chiu HC: Indocyanine green/doxorubicin-encapsulated functionalized nanoparticles for effective combination therapy against human MDR breast cancer. Colloids Surf B Biointerfaces. 177:294–305. 2019. View Article : Google Scholar : PubMed/NCBI