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Article Open Access

CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer

  • Authors:
    • Xin Wang
    • Guowei Cheng
    • Tao Zhang
    • Lei Deng
    • Kunpeng Xu
    • Xin Xu
    • Wenqing Wang
    • Zongmei Zhou
    • Qinfu Feng
    • Dongfu Chen
    • Nan Bi
    • Luhua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China, Department of Radiotherapy, Huanxing Tumor Hospital, Beijing 100023, P.R. China, Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital and Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, Guangdong 518116, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 75-86
    |
    Published online on: October 30, 2019
       https://doi.org/10.3892/or.2019.7395
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Abstract

Esophageal squamous cell carcinoma (ESCC) is a common type of esophageal cancer and is prevalent worldwide. Understanding the mechanisms underlying its formation and the search for more effective therapeutic strategies are critical due to the occurrence of chemotherapeutic drug resistance. The aim of the present study was to determine the functional relevance and therapeutic potential of carbohydrate sulfotransferase 15 (CHST15) in ESCC. CHST15 levels were measured in different ESCC cell lines and evaluated in ESCC tissues using tissue chip immunohistochemistry. Cell growth and apoptosis assays, 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assays, and clonogenic assays were conducted using TE‑1 cells and lenti‑shCHST15 virus constructs were used to investigate the function of CHST15 in cell proliferation and apoptosis. mRNA microarray analysis was performed to determine the underlying mechanism of CHST15 regulation in TE‑1 cell proliferation and apoptosis. The results showed that knockdown of CHST15 inhibited TE‑1 cell growth and proliferation, but induced cell apoptosis. CHST15 was more frequently detected in ESCC tissue compared with that in normal esophageal tissue. Microarray data analysis indicated that the inhibition of cell proliferation and activation of cell apoptosis in CHST15‑knockdown cells may be caused by altered CHST15/ILKAP/CCND1 and CHST15/RABL6/PMAIP1 signaling axes, respectively.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Cheng G, Zhang T, Deng L, Xu K, Xu X, Wang W, Zhou Z, Feng Q, Chen D, Chen D, et al: CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer. Oncol Rep 43: 75-86, 2020.
APA
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X. ... Wang, L. (2020). CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer. Oncology Reports, 43, 75-86. https://doi.org/10.3892/or.2019.7395
MLA
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X., Wang, W., Zhou, Z., Feng, Q., Chen, D., Bi, N., Wang, L."CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer". Oncology Reports 43.1 (2020): 75-86.
Chicago
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X., Wang, W., Zhou, Z., Feng, Q., Chen, D., Bi, N., Wang, L."CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer". Oncology Reports 43, no. 1 (2020): 75-86. https://doi.org/10.3892/or.2019.7395
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Cheng G, Zhang T, Deng L, Xu K, Xu X, Wang W, Zhou Z, Feng Q, Chen D, Chen D, et al: CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer. Oncol Rep 43: 75-86, 2020.
APA
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X. ... Wang, L. (2020). CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer. Oncology Reports, 43, 75-86. https://doi.org/10.3892/or.2019.7395
MLA
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X., Wang, W., Zhou, Z., Feng, Q., Chen, D., Bi, N., Wang, L."CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer". Oncology Reports 43.1 (2020): 75-86.
Chicago
Wang, X., Cheng, G., Zhang, T., Deng, L., Xu, K., Xu, X., Wang, W., Zhou, Z., Feng, Q., Chen, D., Bi, N., Wang, L."CHST15 promotes the proliferation of TE‑1 cells via multiple pathways in esophageal cancer". Oncology Reports 43, no. 1 (2020): 75-86. https://doi.org/10.3892/or.2019.7395
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