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Article Open Access

MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39

  • Authors:
    • Chenxing Li
    • Aiying Wang
    • Yanke Chen
    • Yan Liu
    • Hui Zhang
    • Jun Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Genetics and Cell Biology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Department of Preventive Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, College of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1221-1233
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    Published online on: January 31, 2020
       https://doi.org/10.3892/or.2020.7486
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Abstract

Numerous studies have demonstrated that microRNAs (miRNAs) play a key role in human carcinogenesis and metastasis. For example, miR‑299‑5p has previously been revealed to be dysregulated in several human cancers. However, the biological function of miR‑299‑5p in breast cancer remains unclear. The present study demonstrated that miR‑299‑5p was downregulated in breast cancer tissues and cell lines. The restoration of miR‑299‑5p expression suppressed cell migration and invasion, whereas inhibition of miR‑299‑5p promoted cell migration and invasion. In addition, in vivo studies demonstrated that miR‑299‑5p overexpression was able to inhibit tumour metastasis in nude mice. Mechanistically, through bioinformatics analysis and a dual‑luciferase assay, it was confirmed that miR‑299‑5p directly targets serine/threonine kinase 39 (STK39). Silencing STK39 inhibited cell metastasis and suppressed epithelial‑mesenchymal transition markers and matrix metalloproteinase expression, whereas restoration of STK39 expression was able to reverse miR‑299‑5p‑inhibited cell migration and invasion. Collectively, the results of the present study demonstrated that miR‑299‑5p supresses breast cancer cell migration and invasion by targeting STK39. These findings may provide novel insights into miR‑299‑5p and its potential diagnostic and therapeutic benefits in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li C, Wang A, Chen Y, Liu Y, Zhang H and Zhou J: MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39. Oncol Rep 43: 1221-1233, 2020.
APA
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., & Zhou, J. (2020). MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39. Oncology Reports, 43, 1221-1233. https://doi.org/10.3892/or.2020.7486
MLA
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., Zhou, J."MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39". Oncology Reports 43.4 (2020): 1221-1233.
Chicago
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., Zhou, J."MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39". Oncology Reports 43, no. 4 (2020): 1221-1233. https://doi.org/10.3892/or.2020.7486
Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Wang A, Chen Y, Liu Y, Zhang H and Zhou J: MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39. Oncol Rep 43: 1221-1233, 2020.
APA
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., & Zhou, J. (2020). MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39. Oncology Reports, 43, 1221-1233. https://doi.org/10.3892/or.2020.7486
MLA
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., Zhou, J."MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39". Oncology Reports 43.4 (2020): 1221-1233.
Chicago
Li, C., Wang, A., Chen, Y., Liu, Y., Zhang, H., Zhou, J."MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39". Oncology Reports 43, no. 4 (2020): 1221-1233. https://doi.org/10.3892/or.2020.7486
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