Open Access

Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma

  • Authors:
    • Qilong Wang
    • Bin Han
    • Wu Huang
    • Chunjian Qi
    • Fang Liu
  • View Affiliations

  • Published online on: February 21, 2020     https://doi.org/10.3892/or.2020.7510
  • Pages: 1035-1044
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioma is the most commonly diagnosed primary intracranial malignant tumor with rapid growth, easy recurrence and thus poor prognosis. In the present study, the role of kinesin‑12 (KIF15) in glioma was revealed. Immunohistochemical staining and western blot analysis were used to detect the protein expression. An MTT assay was performed to evaluate cell proliferation. Flow cytometric analysis was utilized to assess cell apoptosis and the cell cycle. A mouse xenograft model was constructed for in vivo study. The results indicated that KIF15 was significantly upregulated in glioma tumor tissues and positively correlated with pathological staging, recurrence risk and poor prognosis. Silencing of KIF15 could inhibit cell proliferation and stemness of glioma cells, arrest cells in the G2 phase and induce cell apoptosis. The in vivo study verified the inhibitory effect of KIF15 knockdown on tumor growth. The mechanism study demonstrated the regulation of apoptosis‑ and cycle‑related proteins in the KIF15 KD‑induced inhibition of glioma. KIF15 was revealed to function as a tumor promoter in the development and progression of glioma. KIF15 also served as a prognostic indicator for glioma and may be a therapeutic target for glioma therapy.
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April-2020
Volume 43 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Wang Q, Han B, Huang W, Qi C and Liu F: Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma. Oncol Rep 43: 1035-1044, 2020
APA
Wang, Q., Han, B., Huang, W., Qi, C., & Liu, F. (2020). Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma. Oncology Reports, 43, 1035-1044. https://doi.org/10.3892/or.2020.7510
MLA
Wang, Q., Han, B., Huang, W., Qi, C., Liu, F."Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma". Oncology Reports 43.4 (2020): 1035-1044.
Chicago
Wang, Q., Han, B., Huang, W., Qi, C., Liu, F."Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma". Oncology Reports 43, no. 4 (2020): 1035-1044. https://doi.org/10.3892/or.2020.7510