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MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer

  • Authors:
    • Cuilan Wang
    • Qing Li
    • Yuan He
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Jinan Maternity and Child Health Care Hospital, Jinan, Shandong 250001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1897-1905
    |
    Published online on: March 20, 2020
       https://doi.org/10.3892/or.2020.7556
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Abstract

MicroRNA (miRNA/miR)‑21‑5p has been proposed as an oncogenic miRNA in human tumors; however, the exact role of miR‑21‑5p has not been fully determined in endometrial cancer. SRY‑box 17 (SOX17) is associated with endometrial cancer development and progression; however, the regulatory mechanisms underlying SOX17 expression in endometrial cancer remain unclear. In the present study, tumor samples were collected from 160 postmenopausal women with endometrial cancer. All tumor samples were examined for miR‑21‑5p expression by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The results demonstrated that miR‑21‑5p expression was associated with shorter overall survival. In addition, overexpression of miR‑21‑5p promoted epithelial to mesenchymal transition (EMT), whereas silencing miR‑21‑5p reversed EMT in endometrial cancer cell lines. Using RT‑qPCR and western blotting, it was revealed that overexpressing miR‑21‑5p significantly inhibited SOX17 protein expression in endometrial cancer cell lines. Furthermore, as determined by luciferase reporter assay, ectopic expression of miR‑21‑5p inhibited the activity of the SOX17 mRNA 3'‑untranslated region (3'UTR), whereas silencing miR‑21‑5p promoted the activity of the SOX17 mRNA 3'UTR in endometrial cancer cell lines. Overexpression of SOX17 promoted mesenchymal to epithelial transition, whereas silencing SOX17 induced EMT in endometrial cancer cell lines. In addition, tumor SOX17 expression was associated with better overall survival. Therefore, it may be concluded that miR‑21‑5p promotes EMT by targeting SOX17 in human endometrial cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Li Q and He Y: MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer. Oncol Rep 43: 1897-1905, 2020.
APA
Wang, C., Li, Q., & He, Y. (2020). MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer. Oncology Reports, 43, 1897-1905. https://doi.org/10.3892/or.2020.7556
MLA
Wang, C., Li, Q., He, Y."MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer". Oncology Reports 43.6 (2020): 1897-1905.
Chicago
Wang, C., Li, Q., He, Y."MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer". Oncology Reports 43, no. 6 (2020): 1897-1905. https://doi.org/10.3892/or.2020.7556
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Li Q and He Y: MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer. Oncol Rep 43: 1897-1905, 2020.
APA
Wang, C., Li, Q., & He, Y. (2020). MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer. Oncology Reports, 43, 1897-1905. https://doi.org/10.3892/or.2020.7556
MLA
Wang, C., Li, Q., He, Y."MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer". Oncology Reports 43.6 (2020): 1897-1905.
Chicago
Wang, C., Li, Q., He, Y."MicroRNA‑21‑5p promotes epithelial to mesenchymal transition by targeting SRY‑box 17 in endometrial cancer". Oncology Reports 43, no. 6 (2020): 1897-1905. https://doi.org/10.3892/or.2020.7556
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