RAD18 promotes colorectal cancer metastasis by activating the epithelial‑mesenchymal transition pathway

Li,Peng; He,Chao; Gao,Aidi; Yan,Xueqi; Xia,Xiaochun; Zhou,Jundong; Wu,Jinchang

doi:10.3892/or.2020.7590

RAD18 promotes colorectal cancer metastasis by activating the epithelial‑mesenchymal transition pathway

Authors:
- Peng Li
- Chao He
- Aidi Gao
- Xueqi Yan
- Xiaochun Xia
- Jundong Zhou
- Jinchang Wu
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Affiliations: Department of Radiation Oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China, Suzhou Cancer Center Core Laboratory, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215001, P.R. China, Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University, Nantong, Jiangsu 226361, P.R. China
Published online on: April 21, 2020 https://doi.org/10.3892/or.2020.7590
Pages: 213-223
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

RAD18 is an E3 ubiquitin‑protein ligase that has a role in carcinogenesis and tumor progression owing to its involvement in error‑prone replication. Despite its significance, the function of RAD18 has not been fully examined in colorectal cancer (CRC). In the present research, by collecting clinical samples and conducting immunohistochemical staining, we found that RAD18 expression was significantly increased in the CRC tissue compared with that noted in the adjacent non‑cancerous normal tissues and that high expression of RAD18 was associated with lymph node metastasis and poor prognosis in CRC patients. In vitro, as determined by cell transfection, scratch, and Transwell experiments, it was also demonstrated that RAD18 increased the invasiveness and migration capacity of CRC cells (HCT116, DLD‑1, SW480). The signaling pathway was analyzed by western blotting and the clinical data were analyzed by immunohistochemical staining and RT‑PCR, indicating that the process of epithelial‑mesenchymal transition (EMT) may be involved in RAD18‑mediated migration and invasion of CRC cells. All of the above data indicate that RAD18 is a novel prognostic biomarker that may become a potential therapeutic target for CRC in the future.

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