Effect of the natural compound trans‑resveratrol on human MCM4 gene transcription

  • Authors:
    • Fumiaki Uchiumi
    • Chihiro Katsuda
    • Motohiro Akui
    • Monami Kusaka
    • Marie Tanaka
    • Masashi Asai
    • Sei‑Ichi Tanuma
  • View Affiliations

  • Published online on: April 28, 2020     https://doi.org/10.3892/or.2020.7598
  • Pages: 283-292
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

trans‑Resveratrol (Rsv) is a natural compound contained in red wine and grape skins that has various beneficial effects for organisms such as lengthening of their life span. Rsv induces expression of the human TP53 and HELB genes, which are involved in the regulation of DNA maintenance. In the present study, a luciferase expression vector containing 309 bp of the 5' upstream end of the human MCM4 gene was transfected into HeLa S3 cells. A luciferase assay revealed that Rsv treatment increased the minichromosome maintenance 4 (MCM4) gene promoter activity by GC‑box and GGAA (TTCC) motifs. Electro phoretic mobility shift assay revealed that the specific binding factor (complex) contains PU.1 (SPI1). Quantitative reverse transcription‑polymerase chain reaction analysis indicated that MCM4 gene expression was transiently induced by Rsv. Moreover, western blotting revealed that the SP1/PU.1 ratio markedly increased after Rsv treatment, indicating that a balance or profile of these transcription factors may control Rsv‑inducible initiation of transcription. These observations indicated that the beneficial effects of Rsv can be attributed to induction of the chromosomal DNA maintenance factor encoding gene expression.
View Figures
View References

Related Articles

Journal Cover

July 2020
Volume 44 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Uchiumi, F., Katsuda, C., Akui, M., Kusaka, M., Tanaka, M., Asai, M., & Tanuma, S. (2020). Effect of the natural compound trans‑resveratrol on human MCM4 gene transcription. Oncology Reports, 44, 283-292. https://doi.org/10.3892/or.2020.7598
MLA
Uchiumi, F., Katsuda, C., Akui, M., Kusaka, M., Tanaka, M., Asai, M., Tanuma, S."Effect of the natural compound trans‑resveratrol on human MCM4 gene transcription". Oncology Reports 44.1 (2020): 283-292.
Chicago
Uchiumi, F., Katsuda, C., Akui, M., Kusaka, M., Tanaka, M., Asai, M., Tanuma, S."Effect of the natural compound trans‑resveratrol on human MCM4 gene transcription". Oncology Reports 44, no. 1 (2020): 283-292. https://doi.org/10.3892/or.2020.7598