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NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis

  • Authors:
    • Xia Zhang
    • Ming‑Xiu Guan
    • Qiu‑Hong Jiang
    • Sai Li
    • Hong‑Yu Zhang
    • Zhi‑Guo Wu
    • Hong‑Liang Cong
    • Xiu‑Hui Qi
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin 301800, P.R. China, Department of Clinical Laboratory, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin 301800, P.R. China, Department of Cardiology, Shenyang Fourth People's Hospital, Shenyang, Liaoning 110031, P.R. China, Department of Cardiovascular Medicine, Tianjin Chest Hospital, Tianjin 300202, P.R. China, Department of Nursing, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin 301800, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 115-125
    |
    Published online on: May 7, 2020
       https://doi.org/10.3892/or.2020.7605
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Abstract

Long non‑coding RNAs (lncRNAs) have been validated to mediate the development of atherosclerosis (AS). In the present study, the molecular mechanisms and functions of lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in the advancement of human aortic endothelial cells (HAECs) were investigated. The levels of lncRNA‑NEAT1 and miR‑638 expression in clinical samples and cells were explored via quantitative reverse transcription polymerase chain reaction. Colony formation and CCK‑8 assays were performed to determine the proliferative capacity of cells, and the apoptotic capacity of cells was analyzed on the basis of apoptotic cell proportion and caspase‑3 activity. Then, the proportion of cells and correlations among phosphoglycerate kinase 1 (PGK1), NEAT1, and miR‑638 were determined through RNA immunoprecipitation and luciferase assays and bioinformatics analysis. Moreover, the expression levels of Ki‑67, proliferating cell nuclear antigen, PGK1, Bax, Bcl‑2, (p)‑mTOR, (p)‑AKT, and β‑catenin were analyzed via western blot analysis. In the serum of patients with AS and HAECs induced by oxidized low‑density lipoprotein (ox‑LDL), the expression level of miR‑638 was decreased, whereas that of NEAT1 was increased. After ox‑LDL therapy, NEAT1 knockdown suppressed HAEC proliferation and stimulated HAEC apoptosis, which could be reversed by the miR‑638 inhibitor. NEAT1 inhibited miR‑638 expression through direct mutual action. The following mechanical investigations revealed that PGK1 was a miR‑638 target, whose expression was increased by NEAT1, a competing endogenous RNA of miR‑638. Additionally, the miR‑638 inhibitor contributed to proliferation and suppressed apoptosis through the activation of the AKT/mTOR signaling pathway in ox‑LDL‑induced HAECs. NEAT1 adjusted the AKT/mTOR signaling pathway via miR‑638 in ox‑LDL‑induced HAECs to accelerate their proliferation and impede their apoptosis. This result revealed that NEAT1 may be valuable in the treatment of AS.
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1 

Laslett LJ, Alagona P Jr, Clark BA III, Drozda JP Jr, Saldivar F, Wilson SR, Poe C and Hart M: The worldwide environment of cardiovascular disease: Prevalence, diagnosis, therapy, and policy issues: A report from the American College of Cardiology. J Am Coll Cardiol. 60 (25 Suppl):S1–S49. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Dharmakidari S, Bhattacharya P and Chaturvedi S: Carotid artery stenosis: Medical therapy, surgery, and stenting. Curr Neurol Neurosci Rep. 17:772017. View Article : Google Scholar : PubMed/NCBI

3 

Libby P, Bornfeldt KE and Tall AR: Atherosclerosis: Successes, surprises, and future challenges. Circ Res. 118:531–534. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Li Y, Yang C, Zhang L and Yang P: MicroRNA-210 induces endothelial cell apoptosis by directly targeting PDK1 in the setting of atherosclerosis. Cell Mol Biol Lett. 22:32017. View Article : Google Scholar : PubMed/NCBI

5 

Chen M, Ren L, Meng Y, Shi L, Chen L, Yu B, Wu Q and Qi G: The protease inhibitor E64d improves ox-LDL-induced endothelial dysfunction in human aortic endothelial cells. Can J Physiol Pharmacol. 96:120–127. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Chen X, Yan CC, Zhang X and You ZH: Long non-coding RNAs and complex diseases: From experimental results to computational models. Brief Bioinform. 18:558–576. 2017.PubMed/NCBI

7 

Liu Y, Zheng L, Wang Q and Hu YW: Emerging roles and mechanisms of long noncoding RNAs in atherosclerosis. Int J Cardiol. 228:570–582. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Pan JX: LncRNA H19 promotes atherosclerosis by regulating MAPK and NF-kB signaling pathway. Eur Rev Med Pharmacol Sci. 21:322–328. 2017.PubMed/NCBI

9 

Shan K, Jiang Q, Wang XQ, Wang YN, Yang H, Yao MD, Liu C, Li XM, Yao J, Liu B, et al: Role of long non-coding RNA-RNCR3 in atherosclerosis-related vascular dysfunction. Cell Death Dis. 7:e22482016. View Article : Google Scholar : PubMed/NCBI

10 

Yu X, Li Z, Zheng H, Chan MT and Wu WK: NEAT1: A novel cancer-related long non-coding RNA. Cell Prolif. 50:2017. View Article : Google Scholar

11 

Huang-Fu N, Cheng JS, Wang Y, Li ZW and Wang SH: Neat1 regulates oxidized low-density lipoprotein-induced inflammation and lipid uptake in macrophages via paraspeckle formation. Mol Med Rep. 17:3092–3098. 2018.PubMed/NCBI

12 

Wang L, Xia JW, Ke ZP and Zhang BH: Blockade of NEAT1 represses inflammation response and lipid uptake via modulating miR-342-3p in human macrophages THP-1 cells. J Cell Physiol. 234:5319–5326. 2019. View Article : Google Scholar : PubMed/NCBI

13 

Rupaimoole R and Slack FJ: MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16:203–222. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Aryal B, Singh AK, Rotllan N, Price N and Fernández-Hernando C: MicroRNAs and lipid metabolism. Curr Opin Lipidol. 28:273–280. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Li M, Wang J and Liu H: Downregulation of miR-638 promotes progression of breast cancer and is associated with prognosis of breast cancer patients. Onco Targets Ther. 11:6871–6877. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Zhang Y, Zhang D, Jiang J and Dong L: Loss of miR-638 promotes invasion and epithelial-mesenchymal transition by targeting SOX2 in hepatocellular carcinoma. Oncol Rep. 37:323–332. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Paraskevopoulou MD and Hatzigeorgiou AG: Analyzing MiRNA-LncRNA Interactions. Methods Mol Biol. 1402:271–286. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, Pienta KJ and Taichman RS: Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression. Cancer Res. 70:471–480. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Weber C and Noels H: Atherosclerosis: Current pathogenesis and therapeutic options. Nat Med. 17:1410–1422. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zhou T, Ding JW, Wang XA and Zheng XX: Long noncoding RNAs and atherosclerosis. Atherosclerosis. 248:51–61. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Zheng DH, Wang X, Lu LN, Chen DL, Chen JM, Lin FM and Xu XB: MiR-638 serves as a tumor suppressor by targeting HOXA9 in glioma. Eur Rev Med Pharmacol Sci. 22:7798–7806. 2018.PubMed/NCBI

22 

Zhao P, Zhang BL, Liu K, Qin B and Li ZH: Overexpression of miR-638 attenuated the effects of hypoxia/reoxygenation treatment on cell viability, cell apoptosis and autophagy by targeting ATG5 in the human cardiomyocytes. Eur Rev Med Pharmacol Sci. 22:8462–8471. 2018.PubMed/NCBI

23 

Nakajima K, Nakano T and Tanaka A: The oxidative modification hypothesis of atherosclerosis: The comparison of atherogenic effects on oxidized LDL and remnant lipoproteins in plasma. Clin Chim Acta. 367:36–47. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Dong Y, Fernandes C, Liu Y, Wu Y, Wu H, Brophy ML, Deng L, Song K, Wen A, Wong S, et al: Role of endoplasmic reticulum stress signalling in diabetic endothelial dysfunction and atherosclerosis. Diab Vasc Dis Res. 14:14–23. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Gimbrone MA Jr and Garcia-cardena G: Endothelial cell dysfunction and the pathobiology of atherosclerosis. Circ Res. 118:620–636. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Hainsworth AH, Oommen AT and Bridges LR: Bridges, Endothelial cells and human cerebral small vessel disease. Brain Pathol. 25:44–50. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Goveia J, Stapor P and Carmeliet P: Principles of targeting endothelial cell metabolism to treat angiogenesis and endothelial cell dysfunction in disease. EMBO Mol Med. 6:1105–1120. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Osorio-Fuentealba C, Contreras-Ferrat AE, Altamirano F, Espinosa A, Li Q, Niu W, Lavandero S, Klip A and Jaimovich E: Electrical stimuli release ATP to increase GLUT4 translocation and glucose uptake via PI3Kγ-Akt-AS160 in skeletal muscle cells. Diabetes. 62:1519–1126. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S and Yamada Y: Pannexin 3 functions as an ER Ca(2+) channel, hemichannel, and gap junction to promote osteoblast differentiation. J Cell Biol. 193:1257–1274. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Cheng A, Wang S, Yang D, Xiao R and Mattson MP: Calmodulin mediates brain-derived neurotrophic factor cell survival signaling upstream of Akt kinase in embryonic neocortical neurons. J Biol Chem. 278:7591–7599. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Lay AJ, Jiang XM, Kisker O, Flynn E, Underwood A, Condron R and Hogg PJ: Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase. Nature. 408:869–873. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Guan MX, Jiang QH, Li S, Zhang HY, Wu ZG, Cong HL and Qi XH: NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis. Oncol Rep 44: 115-125, 2020.
APA
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z. ... Qi, X. (2020). NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis. Oncology Reports, 44, 115-125. https://doi.org/10.3892/or.2020.7605
MLA
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z., Cong, H., Qi, X."NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis". Oncology Reports 44.1 (2020): 115-125.
Chicago
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z., Cong, H., Qi, X."NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis". Oncology Reports 44, no. 1 (2020): 115-125. https://doi.org/10.3892/or.2020.7605
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Guan MX, Jiang QH, Li S, Zhang HY, Wu ZG, Cong HL and Qi XH: NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis. Oncol Rep 44: 115-125, 2020.
APA
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z. ... Qi, X. (2020). NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis. Oncology Reports, 44, 115-125. https://doi.org/10.3892/or.2020.7605
MLA
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z., Cong, H., Qi, X."NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis". Oncology Reports 44.1 (2020): 115-125.
Chicago
Zhang, X., Guan, M., Jiang, Q., Li, S., Zhang, H., Wu, Z., Cong, H., Qi, X."NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR‑638/AKT/mTOR signaling in atherosclerosis". Oncology Reports 44, no. 1 (2020): 115-125. https://doi.org/10.3892/or.2020.7605
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