Open Access

Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial‑mesenchymal transition

  • Authors:
    • Guifeng Xu
    • Ling Ou
    • Ying Liu
    • Xiao Wang
    • Kaisheng Liu
    • Jieling Li
    • Junjun Li
    • Shaoqi Wang
    • Dane Huang
    • Kai Zheng
    • Shaoxiang Wang
  • View Affiliations

  • Published online on: May 7, 2020     https://doi.org/10.3892/or.2020.7606
  • Pages: 29-42
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Matrix metalloproteinases (MMPs) are involved in the cleavage of several components of the extracellular matrix and serve important roles in tumor growth, metastasis and invasion. Previous studies have focused on the expression of one or several MMPs in esophageal squamous cell carcinoma (ESCC); however, in the present study, the transcriptomics of all 23 MMPs were systematically investigated with a focus on the prognostic value of the combination of MMPs. In this study, 8 overlapping differentially expressed genes of the MMP family were identified based on data obtained from Gene Expression Omnibus and The Cancer Genome Atlas. The prognostic value of these MMPs were investigated; the receiver operating characteristic curves, survival curves and nomograms showed that the combination of 6 selected MMPs possessed a good predictive ability, which was more accurate than the prediction model based on Tumor‑Node‑Metastasis stage. Gene set enrichment analysis and gene co‑expression analysis were performed to investigate the potential mechanism of action of MMPs in ESCC. The MMP family was associated with several signaling pathways, such as epithelial‑mesenchymal transition (EMT), Notch, TGF‑β, mTOR and P53. Cell Counting Kit‑8, colony formation, wound healing assays and western blotting were used to determine the effect of BB‑94, a pan‑MMP inhibitor, on proliferation and migration of ESCC cells. BB‑94 treatment decreased ESCC cell growth, migration and EMT. Therefore, MMPs may serve both as diagnostic and prognostic biomarkers of ESCC, and MMP inhibition may be a promising preventive and therapeutic strategy for patients with ESCC.
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July 2020
Volume 44 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Xu, G., Ou, L., Liu, Y., Wang, X., Liu, K., Li, J. ... Wang, S. (2020). Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial‑mesenchymal transition. Oncology Reports, 44, 29-42. https://doi.org/10.3892/or.2020.7606
MLA
Xu, G., Ou, L., Liu, Y., Wang, X., Liu, K., Li, J., Li, J., Wang, S., Huang, D., Zheng, K., Wang, S."Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial‑mesenchymal transition". Oncology Reports 44.1 (2020): 29-42.
Chicago
Xu, G., Ou, L., Liu, Y., Wang, X., Liu, K., Li, J., Li, J., Wang, S., Huang, D., Zheng, K., Wang, S."Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial‑mesenchymal transition". Oncology Reports 44, no. 1 (2020): 29-42. https://doi.org/10.3892/or.2020.7606