Alisol A is potentially therapeutic in human breast cancer cells

Shi,Yanyan; Wang,Mopei; Wang,Pan; Zhang,Ting; Yu,Jingyi; Shi,Lunyong; Li,Mo; Wang,Hao; Zhang,Qingying; Zhao,Hongmei

doi:10.3892/or.2020.7654

Alisol A is potentially therapeutic in human breast cancer cells

Authors:
- Yanyan Shi
- Mopei Wang
- Pan Wang
- Ting Zhang
- Jingyi Yu
- Lunyong Shi
- Mo Li
- Hao Wang
- Qingying Zhang
- Hongmei Zhao
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Affiliations: Peking University Third Hospital, Research Center of Clinical Epidemiology, Beijing 100191, P.R. China, Peking University Third Hospital, Department of Tumor Chemotherapy and Radiation Sickness, Beijing 100191, P.R. China, Peking University Third Hospital, Department of Obstetrics and Gynecology, Beijing 100191, P.R. China, Peking University Health Science Center, Department of Microbiology, Beijing 100191, P.R. China, Peking University Health Science Center, School of Pharmaceutical Sciences, Department of Natural Medicines, Beijing 100191, P.R. China, Sun Yat‑Sen Memorial Hospital of Sun Yat‑Sen University, Department of Obstetrics and Gynecology, Guangzhou, Guangdong 510120, P.R. China, Peking University Third Hospital, Department of General Surgery, Beijing 100191, P.R. China
Published online on: June 22, 2020 https://doi.org/10.3892/or.2020.7654
Pages: 1266-1274

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Abstract

Recent developments in breast cancer therapy have significantly improved patient survival rate; however, recurrence remains a major problem. Systemic treatment of breast cancer with available therapies is not curative. Natural products can be potentially used for treating cancer. Recently, a wide range of pharmacological activities has been reported for Alismatis Rhizoma, a popular traditional Chinese medicine. However, the mechanisms via which its compounds act on breast cancer remain unclear. The present study aimed to investigate the potential of natural therapeutic agents from Alismatis Rhizoma for treating breast cancer. Human breast cancer MDA‑MB‑231 cells were treated with four main protostane triterpenes from Alismatis Rhizoma, including alisol A, alisol A 24‑acetate, alisol B and alisol B 23‑acetate. Among these, alisol A significantly inhibited cell viability. Alisol A induced cell apoptosis, G1 phase cell cycle arrest, autophagy, and intracellular reactive oxygen species (ROS) generation in MDA‑MB‑231 cells. The number of APE1‑/γH2AX‑/LC3‑II positive cells was also significantly higher compared with that of negative control cells. All these results were dose‑dependent. Cleaved caspase‑3, cleaved caspase 9, Bcl‑2, and p‑p38 expression indicated cell apoptosis after alisol A treatment. The changes in cyclin A and cyclin D1 expression was associated with cell cycle arrest upon alisol A treatment. Furthermore, LC3‑II expression upon alisol A treatment was indicative of autophagy. Alisol A treatment can induce autophagy‑dependent apoptosis in human breast cancer cells via induction of ROS and DNA damage. Thus, Alisol A might serve as a new therapeutic agent against breast cancer.

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