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MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2

  • Authors:
    • Jikui Sun
    • Zhijuan Chen
    • Jinbiao Xiong
    • Qiong Wang
    • Fan Tang
    • Xuebin Zhang
    • Lidong Mo
    • Chen Wang
    • Weijia Fan
    • Jinhuan Wang
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Nankai University, Tianjin 300071, P.R. China, Clinical Medicine School, Tianjin Medical University, Tianjin 300070, P.R. China, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory, Tianjin Neurosurgery Institute, Department of Neurosurgery, Tianjin Huan Hu Hospital, Tianjin 300350, P.R. China, Pathology Department, Tianjin Huan Hu Hospital, Tianjin 300350, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2108-2120
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    Published online on: August 19, 2020
       https://doi.org/10.3892/or.2020.7741
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Abstract

MicroRNAs (miRs), which act as crucial regulators of oncogenes and tumor suppressors, have been confirmed to play a significant role in the initiation and progression of various malignancies, including glioma. The present study analyzed the expression and roles of miR‑422a in glioma, and reverse transcription‑quantitative PCR confirmed that miR‑422a expression was significantly lower in glioblastoma multiforme (GBM) samples and cell lines compared with the low‑grade glioma samples and the H4 cell line, respectively. miR‑422a overexpression suppressed proliferation and invasion, and induced apoptosis in LN229 and U87 cell lines. Luciferase reporter assay, western blotting and RNA immunoprecipitation analysis revealed that ribophorin II (RPN2) is a direct functional target of miR‑422a. Additionally, the overexpression of RPN2 partially reversed the miR‑422a‑mediated inhibitory effect on the malignant phenotype. Mechanistic investigation demonstrated that the upregulation of miR‑422a inhibited β‑catenin/transcription factor 4 transcriptional activity, at least partially through RPN2, as indicated by in vitro and in vivo experiments. Furthermore, RPN2 expression was inversely correlated with miR‑422a expression in GBM specimens and predicted patient survival in the Chinese Glioma Genome Atlas, UALCAN, Gene Expression Profiling Interactive Analysis databases. In conclusion, the present data reveal a new miR‑422a/RPN2/Wnt/β‑catenin signaling axis that plays critical roles in glioma tumorigenesis, and it represents a potential therapeutic target for GBM.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Chen Z, Xiong J, Wang Q, Tang F, Zhang X, Mo L, Wang C, Fan W, Wang J, Wang J, et al: MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncol Rep 44: 2108-2120, 2020.
APA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X. ... Wang, J. (2020). MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncology Reports, 44, 2108-2120. https://doi.org/10.3892/or.2020.7741
MLA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44.5 (2020): 2108-2120.
Chicago
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44, no. 5 (2020): 2108-2120. https://doi.org/10.3892/or.2020.7741
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Chen Z, Xiong J, Wang Q, Tang F, Zhang X, Mo L, Wang C, Fan W, Wang J, Wang J, et al: MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncol Rep 44: 2108-2120, 2020.
APA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X. ... Wang, J. (2020). MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncology Reports, 44, 2108-2120. https://doi.org/10.3892/or.2020.7741
MLA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44.5 (2020): 2108-2120.
Chicago
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44, no. 5 (2020): 2108-2120. https://doi.org/10.3892/or.2020.7741
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