Open Access

MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2

  • Authors:
    • Jikui Sun
    • Zhijuan Chen
    • Jinbiao Xiong
    • Qiong Wang
    • Fan Tang
    • Xuebin Zhang
    • Lidong Mo
    • Chen Wang
    • Weijia Fan
    • Jinhuan Wang
  • View Affiliations

  • Published online on: August 19, 2020     https://doi.org/10.3892/or.2020.7741
  • Pages: 2108-2120
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRs), which act as crucial regulators of oncogenes and tumor suppressors, have been confirmed to play a significant role in the initiation and progression of various malignancies, including glioma. The present study analyzed the expression and roles of miR‑422a in glioma, and reverse transcription‑quantitative PCR confirmed that miR‑422a expression was significantly lower in glioblastoma multiforme (GBM) samples and cell lines compared with the low‑grade glioma samples and the H4 cell line, respectively. miR‑422a overexpression suppressed proliferation and invasion, and induced apoptosis in LN229 and U87 cell lines. Luciferase reporter assay, western blotting and RNA immunoprecipitation analysis revealed that ribophorin II (RPN2) is a direct functional target of miR‑422a. Additionally, the overexpression of RPN2 partially reversed the miR‑422a‑mediated inhibitory effect on the malignant phenotype. Mechanistic investigation demonstrated that the upregulation of miR‑422a inhibited β‑catenin/transcription factor 4 transcriptional activity, at least partially through RPN2, as indicated by in vitro and in vivo experiments. Furthermore, RPN2 expression was inversely correlated with miR‑422a expression in GBM specimens and predicted patient survival in the Chinese Glioma Genome Atlas, UALCAN, Gene Expression Profiling Interactive Analysis databases. In conclusion, the present data reveal a new miR‑422a/RPN2/Wnt/β‑catenin signaling axis that plays critical roles in glioma tumorigenesis, and it represents a potential therapeutic target for GBM.
View Figures
View References

Related Articles

Journal Cover

November-2020
Volume 44 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Chen Z, Xiong J, Wang Q, Tang F, Zhang X, Mo L, Wang C, Fan W, Wang J, Wang J, et al: MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncol Rep 44: 2108-2120, 2020
APA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X. ... Wang, J. (2020). MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2. Oncology Reports, 44, 2108-2120. https://doi.org/10.3892/or.2020.7741
MLA
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44.5 (2020): 2108-2120.
Chicago
Sun, J., Chen, Z., Xiong, J., Wang, Q., Tang, F., Zhang, X., Mo, L., Wang, C., Fan, W., Wang, J."MicroRNA‑422a functions as a tumor suppressor in glioma by regulating the Wnt/β‑catenin signaling pathway via RPN2". Oncology Reports 44, no. 5 (2020): 2108-2120. https://doi.org/10.3892/or.2020.7741