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Article

N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1

  • Authors:
    • Guifang He
    • Mingfeng Li
    • Linna Fang
    • Lingfan Xu
    • Xiaojuan Huang
    • Lulu Zheng
    • Lijie Yang
    • Wenwu Luo
    • Yongping Cai
    • Wei Ma
    • Chaozhao Liang
    • Yu Yin
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Urology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Pathology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
  • Pages: 2265-2274
    |
    Published online on: September 22, 2020
       https://doi.org/10.3892/or.2020.7775
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Abstract

The oncoprotein N‑Myc has a carcinogenic effect in numerous types of cancer, and it can cause castration resistance in prostate cancer (PCa), and leads to the development of small cell neuroendocrine cancer by regulating multiple target genes. Immunohistochemical staining, RT‑qPCR, western blotting, wound healing and CCK‑8 assays were used to detect the expression of N‑Myc and FSCN1 as well as AR and CgA at the human level and cell level. The immunohistochemical results revealed that the protein levels of N‑Myc proto‑oncogene protein (N‑Myc) and fascin (FSCN1) in PCa were significantly higher than that of hyperplastic tissues (P<0.05), and there was a weak correlation between them (P=0.002). In vitro, N‑Myc and FSCN1 were overexpressed in LNCaP and C4‑2 cell lines. The results revealed the promoting effect of N‑Myc and FSCN1 on malignant progression of PCa. In addition, the endogenous FSCN1 was knocked down in the C4‑2 cell line, and the results revealed that the silencing of FSCN1 enhanced the expression of N‑Myc and weakened the expression of the neuroendocrine marker CgA. Therefore, the present findings indicated that N‑Myc may promote the malignant process of PCa by regulating FSCN1 and FSCN1 may have a reverse regulatory effect on N‑Myc.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2019. CA Cancer J Clin. 69:7–34. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer Statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Chen Y, Sawyers CL and Scher HI: Targeting the androgen receptor pathway in prostate cancer. Curr Opin Pharmacol. 8:440–448. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Taylor BS, Schultz N, Hieronymus H, Gopalan A, Xiao Y, Carver BS, Arora VK, Kaushik P, Cerami E, Reva B, et al: Integrative genomic profiling of human prostate cancer. Cancer Cell. 18:11–22. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Zarour L and Alumkal J: Emerging therapies in Castrate-resistant prostate cancer. Curr Urol Rep. 11:152–158. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Wang HT, Yao YH, Li BG, Tang Y, Chang JW and Zhang J: Neuroendocrine prostate cancer (NEPC) progressing from conventional prostatic adenocarcinoma: Factors associated with time to development of NEPC and survival from NEPC diagnosis-a systematic review and pooled analysis. J Clin Oncol. 32:3383–3390. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Lotan TL, Gupta NS, Wang W, Toubaji A, Haffner MC, Chaux A, Hicks JL, Meeker AK, Bieberich CJ, De Marzo AM, et al: ERG gene rearrangements are common in prostatic small cell carcinomas. Mod Pathol. 24:820–828. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Tan HL, Sood A, Rahimi HA, Wang W, Gupta N, Hicks J, Mosier S, Gocke CD, Epstein JI, Netto GJ, et al: Rb loss is characteristic of prostatic small cell neuroendocrine carcinoma. Clin Cancer Res. 20:890–903. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Robinson D, Van Allen EM, Wu YM, Schultz N, Lonigro RJ, Mosquera JM, Montgomery B, Taplin ME, Pritchard CC, Attard G, et al: Integrative clinical genomics of advanced prostate cancer. Cell. 161:1215–1228. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Beltran H, Prandi D, Mosquera JM, Benelli M, Puca L, Cyrta J, Marotz C, Giannopoulou E, Chakravarthi BV, Varambally S, et al: Divergent clonal evolution of castration-resistant neuroendocrine prostate cancer. Nat Med. 22:298–305. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Dang CV: MYC on the path to cancer. Cell. 149:22–35. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Schneiderman J, London WB, Brodeur GM, Castleberry RP, Look AT and Cohn SL: Clinical significance of MYCN amplification and ploidy in favorable-stage neuroblastoma: A report from the Children's Oncology Group. J Clin Oncol. 26:913–918. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Massimino M, Biassoni V, Gandola L, Garrè ML, Gatta G, Giangaspero F, Poggi G and Rutkowski S: Childhood medulloblastoma. Crit Rev Oncol Hematol. 105:35–51. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Northcott PA, Jones DT, Kool M, Robinson GW, Gilbertson RJ, Cho YJ, Pomeroy SL, Korshunov A, Lichter P, Taylor MD and Pfister SM: Medulloblastomics: The end of the beginning. Nat Rev Cancer. 12:818–834. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Bjerke L, Mackay A, Nandhabalan M, Burford A, Jury A, Popov S, Bax DA, Carvalho D, Taylor KR, Vinci M, et al: Histone H3.3. Mutations drive pediatric glioblastoma through upregulation of MYCN. Cancer Discov. 3:512–519. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Swartling FJ, Savov V, Persson AI, Chen J, Hackett CS, Northcott PA, Grimmer MR, Lau J, Chesler L, Perry A, et al: Distinct neural stem cell populations give rise to disparate brain tumors in response to N-MYC. Cancer Cell. 21:601–613. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Fielitz K, Althoff K, De Preter K, Nonnekens J, Ohli J, Elges S, Hartmann W, Klöppel G, Knösel T, Schulte M, et al: Characterization of pancreatic glucagon-producing tumors and pituitary gland tumors in transgenic mice overexpressing MYCN in hGFAP-positive cells. Oncotarget. 7:74415–74426. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Yu Y, Xu L, Chang Y, Zeng T, Chen X, Wang A, Groth J, Foo WC, Liang C, Hu H and Huang J: N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway. Mol Cancer. 18:112019. View Article : Google Scholar : PubMed/NCBI

19 

Hanker LC, Karn T, Holtrich U, Graeser M, Becker S, Reinhard J, Ruckhäberle E, Gevensleben H and Rody A: Prognostic impact of fascin-1 (FSCN1) in epithelial ovarian cancer. Anticancer Res. 33:371–377. 2013.PubMed/NCBI

20 

Hashimoto Y, Skacel M and Adams JC: Roles of fascin in human carcinoma motility and signaling: Prospects for a novel biomarker? Int J Biochem Cell Biol. 37:1787–1804. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA, Min C, Jaskelioff M, Xiao Y, Wu CJ, et al: Proinvasion metastasis drivers in Early-stage melanoma are oncogenes. Cancer Cell. 20:92–103. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Li A, Morton JP, Ma Y, Karim SA, Zhou Y, Faller WJ, Woodham EF, Morris HT, Stevenson RP, Juin A, et al: Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes. Gastroenterology. 146:1386–1396.e17. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Barnawi R, Al-Khaldi S, Majed Sleiman G, Sarkar A, Al-Dhfyan A, Al-Mohanna F, Ghebeh H and Al-Alwan M: Fascin is critical for the maintenance of breast cancer stem cell pool predominantly via the activation of the notch Self-renewal pathway. Stem Cells. 34:2799–2813. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Sun J, He H, Xiong Y, Lu S, Shen J, Cheng A, Chang WC, Hou MF, Lancaster JM, Kim M and Yang S: Fascin protein is critical for transforming growth factor β Protein-induced invasion and filopodia formation in Spindle-shaped tumor cells. J Biol Chem. 286:38865–38875. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Snyder M, Huang XY and Zhang JJ: Signal transducers and activators of transcription 3 (STAT3) directly regulates cytokine-induced fascin expression and is required for breast cancer cell migration. J Biol Chem. 286:38886–38893. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Zhao X, Gao S, Ren H, Sun W, Zhang H, Sun J, Yang S and Hao J: Hypoxia-inducible factor-1 promotes pancreatic ductal adenocarcinoma invasion and metastasis by activating transcription of the actin-bundling protein fascin. Cancer Res. 74:2455–2464. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Sandelin A, Wasserman WW and Lenhard B: ConSite: Web-based prediction of regulatory elements using cross-species comparison. Nucleic Acids Res. 32:W249–W252. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Beltran H, Rickman DS, Park K, Chae SS, Sboner A, MacDonald TY, Wang Y, Sheikh KL, Terry S, Tagawa ST, et al: Molecular characterization of neuroendocrine prostate cancer and identification of new drug targets. Cancer Discov. 1:487–495. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Dardenne E, Beltran H, Benelli M, Gayvert K, Berger A, Puca L, Cyrta J, Sboner A, Noorzad Z, MacDonald T, et al: N-Myc induces an EZH2-mediated transcriptional program driving neuroendocrine prostate cancer. Cancer Cell. 30:563–577. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Lee JK, Phillips JW, Smith BA, Park JW, Stoyanova T, McCaffrey EF, Baertsch R, Sokolov A, Meyerowitz JG, Mathis C, et al: N-myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Cancer Cell. 29:536–547. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Beltran H: The N-myc Oncogene: Maximizing its targets, regulation, and therapeutic potential. Mol Cancer Res. 12:815–822. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Adams JC: Roles of fascin in cell adhesion and motility. Curr Opin Cell Biol. 16:590–596. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Lin S, Huang C, Gunda V, Sun J, Chellappan SP, Li Z, Izumi V, Fang B, Koomen J, Singh PK, et al: Fascin controls metastatic colonization and mitochondrial oxidative phosphorylation by remodeling mitochondrial actin filaments. Cell Rep. 28:2824–2836.e8. 2019. View Article : Google Scholar : PubMed/NCBI

35 

Darnel AD, Behmoaram E, Vollmer RT, Corcos J, Bijian K, Sircar K, Su J, Jiao J, Alaoui-Jamali MA and Bismar TA: Fascin regulates prostate cancer cell invasionand is associated with metastasis and biochemical failurein prostate cancer. Cin Cancer Res. 15:1376–1383. 2009. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
He G, Li M, Fang L, Xu L, Huang X, Zheng L, Yang L, Luo W, Cai Y, Ma W, Ma W, et al: N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1. Oncol Rep 44: 2265-2274, 2020.
APA
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L. ... Yin, Y. (2020). N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1. Oncology Reports, 44, 2265-2274. https://doi.org/10.3892/or.2020.7775
MLA
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L., Yang, L., Luo, W., Cai, Y., Ma, W., Liang, C., Yin, Y."N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1". Oncology Reports 44.5 (2020): 2265-2274.
Chicago
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L., Yang, L., Luo, W., Cai, Y., Ma, W., Liang, C., Yin, Y."N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1". Oncology Reports 44, no. 5 (2020): 2265-2274. https://doi.org/10.3892/or.2020.7775
Copy and paste a formatted citation
x
Spandidos Publications style
He G, Li M, Fang L, Xu L, Huang X, Zheng L, Yang L, Luo W, Cai Y, Ma W, Ma W, et al: N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1. Oncol Rep 44: 2265-2274, 2020.
APA
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L. ... Yin, Y. (2020). N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1. Oncology Reports, 44, 2265-2274. https://doi.org/10.3892/or.2020.7775
MLA
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L., Yang, L., Luo, W., Cai, Y., Ma, W., Liang, C., Yin, Y."N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1". Oncology Reports 44.5 (2020): 2265-2274.
Chicago
He, G., Li, M., Fang, L., Xu, L., Huang, X., Zheng, L., Yang, L., Luo, W., Cai, Y., Ma, W., Liang, C., Yin, Y."N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1". Oncology Reports 44, no. 5 (2020): 2265-2274. https://doi.org/10.3892/or.2020.7775
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