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Article

Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines

  • Authors:
    • Hidenori Takahashi
    • Toshiya Kamiyama
    • Nozomi Hirane
    • Nozomi Kobayashi
    • Takeshi Aiyama
    • Akihisa Nagatsu
    • Shingo Shimada
    • Tatsuya Orimo
    • Tatsuhiko Kakisaka
    • Moto Fukai
    • Hideki Yokoo
    • Hirofumi Kamachi
    • Shin‑Ichiro Nishimura
    • Akinobu Taketomi
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery I, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060‑8638, Japan, Graduate School of Life Science and Frontier Research Center for Post‑Genome Science and Technology, Hokkaido University, Sapporo, Hokkaido 001‑0021, Japan
  • Pages: 2757-2769
    |
    Published online on: October 8, 2020
       https://doi.org/10.3892/or.2020.7795
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Abstract

The N‑glycoforms of glycoproteins modify protein function and control a number of biological pathways. The aim of the present study was to investigate the correlation between alterations in N‑glycans and cancer aggressiveness in terms of cancer cell invasion ability. The expression of urokinase‑type plasminogen activator (uPA) and N‑acetylglucosaminyltransferase V (GnT‑V) in liver cancer cell lines was analyzed by western blotting. Cell invasiveness was analyzed by Matrigel invasion assays. uPA and GnT‑V expression in liver cancer cell lines was knocked down by RNA interference. Furthermore, uPA was overexpressed in liver cancer cells using lentiviral vectors, and a mutant strain of HepG2 cells overexpressing uPA deficient in N‑glycans was established. A glycoblotting‑assisted matrix‑assisted laser desorption/ionization‑time‑of‑flight/mass spectrometry‑based quantitative analysis of liver cancer cell lines was performed, in which invasiveness was altered by modifying the expression of uPA and GnT‑V. N‑glycan profiles were found to differ between the highly invasive liver cancer cell line HLE and the less invasive cell line HepG2. The expression of several N‑glycans, including a form with m/z=1892, was changed according to invasiveness controlled by knockdown and overexpression of uPA. The invasiveness of HepG2 cells with mutant uPA did not increase regardless of the level of expression of uPA. Following GnT‑V knockdown and N‑glycan alteration, uPA expression did not change, whereas cell invasiveness decreased. One N‑glycan (m/z=1892) was common among N‑glycans in the comparative analysis between HLE and HepG2, HLE and uPA knockdown HLE, HepG2 and uPA‑overexpressing HepG2, and HLE and GnT‑V knockdown HLE cells and among N‑glycan profiles in human uPA. Therefore, N‑glycosylation is an important factor controlling invasiveness of liver cancer cells, and a specific N‑glycan (m/z=1892) associated with the invasion of liver cancer cells via uPA was identified in the present study.
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View References

1 

Pilobello KT and Mahal LK: Deciphering the glycocode: The complexity and analytical challenge of glycomics. Curr Opin Chem Biol. 11:300–305. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Ohtsubo K and Marth JD: Glycosylation in cellular mechanisms of health and disease. Cell. 126:855–867. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Helenius A and Aebi M: Intracellular functions of N-linked glycans. Science. 291:2364–2369. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Lin G, Zhao R, Wang Y, Han J, Gu Y, Pan Y, Ren C, Ren S and Xu C: Dynamic analysis of N-glycomic and transcriptomic changes in the development of ovarian cancer cell line A2780 to its three cisplatin-resistant variants. Ann Transl Med. 8:2892020. View Article : Google Scholar : PubMed/NCBI

5 

Stephanie H, Jennifer LW, Kamila K, Yoann R and Manfred W: N-glycomic and transcriptomic changes associated with CDX1 mRNA expression in colorectal cancer cell lines. Cells. 8:2732019. View Article : Google Scholar

6 

Yue W, Feifei X, Yun C and Zhixin T: A quantitative N-glycoproteomics study of cell-surface N-glycoprotein markers of MCF-7/ADR cancer stem cells. Anal Bioanal Chem. 412:2423–2432. 2020. View Article : Google Scholar : PubMed/NCBI

7 

Miura Y, Hato M, Shinohara Y, Kuramoto H, Furukawa J, Kurogochi M, Shimaoka H, Tada M, Nakanishi K, Ozaki M, et al: BlotGlycoABCTM, an integrated glycoblotting technique for rapid and large scale clinical glycomics. Mol Cell Proteomics. 7:370–377. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Kamiyama T, Yokoo H, Furukawa J, Kurogochi M, Togashi T, Miura N, Nakanishi K, Kamachi H, Kakisaka T, Tsuruga Y, et al: Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis. Hepatology. 57:2314–2325. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Furukawa J, Tsuda M, Okada K, Kimura T, Piao J, Tanaka S and Shinohara Y: Comprehensive glycomics of a multistep human brain tumor model reveals specific glycosylation patterns related to malignancy. PLoS One. 10:e01283002015. View Article : Google Scholar : PubMed/NCBI

10 

Kang P, Madera M, Alley WR Jr, Goldman R, Mechref Y and Novotny MV: Glycomic alterations in the highly-abundant and lesser-abundant blood serum protein fractions for patients diagnosed with hepatocellular carcinoma. Int J Mass Spectrom. 305:185–198. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Vanhooren V, Liu XE, Franceschi C, Gao CF, Libert C, Contreras R and Chen C: N-glycan profiles as tools in diagnosis of hepatocellular carcinoma and prediction of healthy human ageing. Mech Ageing Dev. 130:92–97. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Kirmiz C, Li B, An HJ, Clowers BH, Chew HK, Lam KS, Ferrige A, Alecio R, Borowsky AD, Sulaimon S, et al: A serum glycomics approach to breast cancer biomarkers. Mol Cell Proteomics. 6:43–55. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Alley WR Jr, Vasseur JA, Goetz JA, Svoboda M, Mann BF, Matei DE, Menning N, Hussein A, Mechref Y and Novotny MV: N-linked glycan structures and their expressions change in the blood sera of ovarian cancer patients. J Proteome Res. 11:2282–2300. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lee SB, Bose S, Ahn SH, Son BH, Ko BS, Kim HJ, Chung IY, Kim J, Lee W, Ko MS, et al: Breast cancer diagnosis by analysis of serum N-glycans using MALDI-TOF mass spectroscopy. PLoS One. 15:e02310042020. View Article : Google Scholar : PubMed/NCBI

15 

Mekkawy AH, Pourgholami MH and Morris DL: Involvement of urokinase-type plasminogen activator system in cancer: An overview. Med Res Rev. 34:918–956. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Zhou Q, Liang LJ, Peng BG and Zhen YY: Expression and clinical significance of coagulate and fibrolysis factors in tissue and plasma from hepatocellular carcinoma patients. Ai Zheng. 25:1433–1438. 2006.(In Chinese). PubMed/NCBI

17 

Itoh T, Hayashi Y, Kanamaru T, Morita Y, Suzuki S, Wang W, Zhou L, Rui JA, Yamamoto M, Kuroda Y and Itoh H: Clinical significance of urokinase-type plasminogen activator activity in hepatocellular carcinoma. J Gastroenterol Hepatol. 15:422–430. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Kamiyama T, Une Y, Uchino J and Hamada J: Hepatocyte growth factor enhances the invasion activity of human hepatocellular carcinoma cell lines. Int J Oncol. 12:655–659. 1998.PubMed/NCBI

19 

Chakraborty AK and Pawelek JM: GnT-V, macrophage and cancer metastasis: A common link. Clin Exp Metastasis. 20:365–373. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Handerson T, Camp R, Harigopal M, Rimm D and Pawelek J: Beta1,6-branched oligosaccharides are increased in lymph node metastases and predict poor outcome in breast carcinoma. Clin Cancer Res. 11:2969–2973. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Handerson T and Pawelek JM: Beta1,6-branched oligosaccharides and coarse vesicles: A common, pervasive phenotype in melanoma and other human cancers. Cancer Res. 63:5363–5369. 2003.PubMed/NCBI

22 

Lee SL, Dickson RB and Lin CY: Activation of hepatocyte growth factor and urokinase/plasminogen activator by matriptase, an epithelial membrane serine protease. J Biol Chem. 275:36720–36725. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Takeuchi T, Harris JL, Huang W, Yan KW, Coughlin SR and Craik CS: Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem. 275:26333–26342. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Ito Y, Akinaga A, Yamanaka K, Nakagawa T, Kondo A, Dickson RB, Lin CY, Miyauchi A, Taniguchi N and Miyoshi E: Co-expression of matriptase and N-acetylglucosaminyltransferase V in thyroid cancer tissues-its possible role in prolonged stability in vivo by aberrant glycosylation. Glycobiology. 16:368–374. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, et al: Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers. Proc Natl Acad Sci USA. 110:2105–2110. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Henkin J, Marcotte P and Yang HC: The plasminogen-plasmin system. Prog Cardiovasc Dis. 34:135–164. 1991. View Article : Google Scholar : PubMed/NCBI

27 

Appella E, Robinson EA, Ullrich SJ, Stoppelli MP, Corti A, Cassani G and Blasi F: The receptor-binding sequence of urokinase. A biological function for the growth-factor module of proteases. J Biol Chem. 262:4437–4440. 1987.PubMed/NCBI

28 

Buko AM, Kentzer EJ, Petros A, Menon G, Zuiderweg ER and Sarin VK: Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator. Proc Natl Acad Sci USA. 88:3992–3996. 1991. View Article : Google Scholar : PubMed/NCBI

29 

Bergwerff AA, Van Oostrum J, Kamerling JP and Vliegenthart JF: The major N-linked carbohydrate chains from human urokinase. The occurrence of 4-O-sulfated, (alpha 2–6)-sialylated or (alpha 1–3)-fucosylated N-acetylgalactosamine(beta 1–4)-N-acetylglucosamine elements. Eur J Biochem. 228:1009–1019. 1995. View Article : Google Scholar : PubMed/NCBI

30 

Kasai S, Arimura H, Nishida M and Suyama T: Primary structure of single-chain pro-urokinase. J Biol Chem. 260:12382–12389. 1985.PubMed/NCBI

31 

McLellan WL, Vetterlein D and Roblin R: The glycoprotein nature of human plasminogen activators. FEBS Lett. 115:181–184. 1980. View Article : Google Scholar : PubMed/NCBI

32 

Sarubbi E, Nolli ML, Robbiati F, Soffientini A, Parenti F and Cassani G: The differential glycosylation of human pro-urokinase from various recombinant mammalian cell lines does not affect activity and binding to PAI-1. Thromb Haemost. 62:927–933. 1989. View Article : Google Scholar : PubMed/NCBI

33 

Li XK, Lijnen HR, Nelles L, Hu MH and Collen D: Biochemical properties of recombinant mutants of nonglycosylated single chain urokinase-type plasminogen activator. Biochim Biophys Acta. 1159:37–43. 1992. View Article : Google Scholar : PubMed/NCBI

34 

Saito H, Nishikawa A, Gu J, Ihara Y, Soejima H, Wada Y, Sekiya C, Niikawa N and Taniguchi N: cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+. Biochem Biophys Res Commun. 198:318–327. 1994. View Article : Google Scholar : PubMed/NCBI

35 

Miyoshi E, Terao M and Kamada Y: Physiological roles of N-acetylglucosaminyltransferase V(GnT-V) in mice. BMB Rep. 45:554–559. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Taniguchi N, Miyoshi E, Ko JH, Ikeda Y and Ihara Y: Implication of N-acetylglucosaminyltransferases III and V in cancer: Gene regulation and signaling mechanism. Biochim Biophys Acta. 1455:287–300. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Cui J, Huang W, Wu B, Jin J, Jing L, Shi WP, Liu ZY, Yuan L, Luo D, Li L, et al: N-glycosylation by N-acetylglucosaminyltransferase V enhances the interaction of CD147/basigin with integrin β1 and promotes HCC metastasis. J Pathol. 245:41–52. 2018. View Article : Google Scholar : PubMed/NCBI

38 

Murata K, Miyoshi E, Kameyama M, Ishikawa O, Kabuto T, Sasaki Y, Hiratsuka M, Ohigashi H, Ishiguro S, Ito S, et al: Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis. Clin Cancer Res. 6:1772–1777. 2000.PubMed/NCBI

39 

Mehta A, Norton P, Liang H, Comunale MA, Wang M, Rodemich-Betesh L, Koszycki A, Noda K, Miyoshi E and Block T: Increased levels of tetra-antennary N-linked glycan but not core fucosylation are associated with hepatocellular carcinoma tissue. Cancer Epidemiol Biomarkers Prev. 21:925–933. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Ito Y, Miyoshi E, Sakon M, Takeda T, Noda K, Tsujimoto M, Ito S, Honda H, Takemura F, Wakasa K, et al: Elevated expression of UDP-N-acetylglucosamine: Alphamannoside beta1,6 N-acetylglucosaminyltransferase is an early event in hepatocarcinogenesis. Int J Cancer. 91:631–637. 2001. View Article : Google Scholar : PubMed/NCBI

41 

Tian H, Miyoshi E, Kawaguchi N, Shaker M, Ito Y, Taniguchi N, Tsujimoto M and Matsuura N: The implication of N-acetylglucosaminyltransferase V expression in gastric cancer. Pathobiology. 75:288–294. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Yamamoto E, Ino K, Miyoshi E, Shibata K, Takahashi N, Kajiyama H, Nawa A, Nomura S, Nagasaka T and Kikkawa F: Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis. Br J Cancer. 97:1538–1544. 2007. View Article : Google Scholar : PubMed/NCBI

43 

Takahashi N, Yamamoto E, Ino K, Miyoshi E, Nagasaka T, Kajiyama H, Shibata K, Nawa A and Kikkawa F: High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary. Oncol Rep. 22:1027–1032. 2009.PubMed/NCBI

44 

Ihara S, Miyoshi E, Ko JH, Murata K, Nakahara S, Honke K, Dickson RB, Lin CY and Taniguchi N: Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1–6 GlcNAc branching. J Biol Chem. 277:16960–16967. 2002. View Article : Google Scholar : PubMed/NCBI

45 

Ihara S, Miyoshi E, Nakahara S, Sakiyama H, Ihara H, Akinaga A, Honke K, Dickson RB, Lin CY and Taniguchi N: Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin. Glycobiology. 14:139–146. 2004. View Article : Google Scholar : PubMed/NCBI

46 

Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N and Yamaguchi Y: Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V. Nat Commun. 9:33802018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Takahashi H, Kamiyama T, Hirane N, Kobayashi N, Aiyama T, Nagatsu A, Shimada S, Orimo T, Kakisaka T, Fukai M, Fukai M, et al: Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines. Oncol Rep 44: 2757-2769, 2020.
APA
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A. ... Taketomi, A. (2020). Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines. Oncology Reports, 44, 2757-2769. https://doi.org/10.3892/or.2020.7795
MLA
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A., Shimada, S., Orimo, T., Kakisaka, T., Fukai, M., Yokoo, H., Kamachi, H., Nishimura, S., Taketomi, A."Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines". Oncology Reports 44.6 (2020): 2757-2769.
Chicago
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A., Shimada, S., Orimo, T., Kakisaka, T., Fukai, M., Yokoo, H., Kamachi, H., Nishimura, S., Taketomi, A."Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines". Oncology Reports 44, no. 6 (2020): 2757-2769. https://doi.org/10.3892/or.2020.7795
Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi H, Kamiyama T, Hirane N, Kobayashi N, Aiyama T, Nagatsu A, Shimada S, Orimo T, Kakisaka T, Fukai M, Fukai M, et al: Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines. Oncol Rep 44: 2757-2769, 2020.
APA
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A. ... Taketomi, A. (2020). Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines. Oncology Reports, 44, 2757-2769. https://doi.org/10.3892/or.2020.7795
MLA
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A., Shimada, S., Orimo, T., Kakisaka, T., Fukai, M., Yokoo, H., Kamachi, H., Nishimura, S., Taketomi, A."Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines". Oncology Reports 44.6 (2020): 2757-2769.
Chicago
Takahashi, H., Kamiyama, T., Hirane, N., Kobayashi, N., Aiyama, T., Nagatsu, A., Shimada, S., Orimo, T., Kakisaka, T., Fukai, M., Yokoo, H., Kamachi, H., Nishimura, S., Taketomi, A."Analysis of the correlation between alterations in N‑glycans and invasiveness in liver cancer cell lines". Oncology Reports 44, no. 6 (2020): 2757-2769. https://doi.org/10.3892/or.2020.7795
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