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Article Open Access

Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer

  • Authors:
    • Cheng Chen
    • Hongjin Wu
    • Deshengyue Kong
    • Yu Xu
    • Zunyue Zhang
    • Fengrong Chen
    • Lei Zou
    • Ziwei Li
    • Jin Shui
    • Huayou Luo
    • Shi‑He Liu
    • Juehua Yu
    • Kunhua Wang
    • F. Charles Brunicardi
  • View Affiliations / Copyright

    Affiliations: The NHC Key Laboratory of Drug Addiction Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China, Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China, Shanghai International Travel Healthcare Center, Shanghai 200000, P.R. China, Department of Surgery, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USA
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2569-2580
    |
    Published online on: October 15, 2020
       https://doi.org/10.3892/or.2020.7810
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Abstract

Statins, a class of commonly prescribed cholesterol‑lowering medications, have been revealed to influence the risk of multiple types of cancer. However, the antitumor effects of statins on pancreatic cancer and their differential efficacy among a variety of statins are not currently well‑defined. The aim of the present study was therefore to identify and compare the genes and related biological pathways that were affected by each individual statin on pancreatic cancer. Two human pancreatic cancer cell lines, MiaPaCa2 and PANC1, were exposed to three statins, lovastatin, fluvastatin and simvastatin. The inhibitory effect of statins on pancreatic cancer cell proliferation was first validated. Next, RNA‑seq analysis was used to determine the gene expression alterations in either low (2 µM) or high (20 µM) statin concentration‑treated cancer cells. Marked differences in gene transcription profiles of both pancreatic cancer cell lines exposed to high concentration statins were observed. Notably, the high concentration statins significantly suppressed core‑gene CCNA2‑associated cell cycle and DNA replication pathways and upregulated genes involved in ribosome and autophagy pathways. However, the low concentration statin‑induced gene expression alterations were only detected in MiaPaCa2 cells. In conclusion, a marked difference in the intra and inter cell‑type performance of pancreatic cancer cells exposed to a variety of statins at low or high concentrations was reported herein, which may provide insights for the potential clinical use of statins in future pancreatic cancer therapeutics.
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Copy and paste a formatted citation
Spandidos Publications style
Chen C, Wu H, Kong D, Xu Y, Zhang Z, Chen F, Zou L, Li Z, Shui J, Luo H, Luo H, et al: Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer. Oncol Rep 44: 2569-2580, 2020.
APA
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F. ... Brunicardi, F.C. (2020). Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer. Oncology Reports, 44, 2569-2580. https://doi.org/10.3892/or.2020.7810
MLA
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F., Zou, L., Li, Z., Shui, J., Luo, H., Liu, S., Yu, J., Wang, K., Brunicardi, F. C."Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer". Oncology Reports 44.6 (2020): 2569-2580.
Chicago
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F., Zou, L., Li, Z., Shui, J., Luo, H., Liu, S., Yu, J., Wang, K., Brunicardi, F. C."Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer". Oncology Reports 44, no. 6 (2020): 2569-2580. https://doi.org/10.3892/or.2020.7810
Copy and paste a formatted citation
x
Spandidos Publications style
Chen C, Wu H, Kong D, Xu Y, Zhang Z, Chen F, Zou L, Li Z, Shui J, Luo H, Luo H, et al: Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer. Oncol Rep 44: 2569-2580, 2020.
APA
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F. ... Brunicardi, F.C. (2020). Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer. Oncology Reports, 44, 2569-2580. https://doi.org/10.3892/or.2020.7810
MLA
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F., Zou, L., Li, Z., Shui, J., Luo, H., Liu, S., Yu, J., Wang, K., Brunicardi, F. C."Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer". Oncology Reports 44.6 (2020): 2569-2580.
Chicago
Chen, C., Wu, H., Kong, D., Xu, Y., Zhang, Z., Chen, F., Zou, L., Li, Z., Shui, J., Luo, H., Liu, S., Yu, J., Wang, K., Brunicardi, F. C."Transcriptome sequencing analysis reveals unique and shared antitumor effects of three statins in pancreatic cancer". Oncology Reports 44, no. 6 (2020): 2569-2580. https://doi.org/10.3892/or.2020.7810
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