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Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11

  • Authors:
    • Daxiang Chen
    • Ruixue Wang
    • Mingjian Long
    • Wei Li
    • Bin Xiao
    • Hao Deng
    • Kongyan Weng
    • Daoyuan Gong
    • Fang Liu
    • Shuhong Luo
    • Wenbo Hao
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Laboratory Medicine, School of Stomatology and Medicine, Foshan University, Foshan, Guangdong 528000, P.R. China, Department of Laboratory Medicine, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510010, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 535-546
    |
    Published online on: December 8, 2020
       https://doi.org/10.3892/or.2020.7885
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Abstract

Orf virus (ORFV) is a favorable oncolytic viral carrier in research, and ORFV strain NZ2 has been revealed to have antitumor effects in animal models mediated by immunoregulation profile. However, the antitumor effects triggered by the ORFV in colorectal cancer (CRC) cells is poorly characterized. The in vivo and in vitro roles of ORFV in CRC were determined using western blotting, colony formation, CCK‑8, wound scratch assay, qPCR, and animal models. Furthermore, cytokine antibody chip assay, flow cytometry, western blotting, and immunohistochemical (IHC) assays were conducted to explore the potential mechanism of ORFV. The present data revealed that ORFV strain NA1/11 infected and inhibited the in vitro growth and migration of CRC cells. By establishing a CRC model in Balb/c mice, it was revealed that ORFV strain NA1/11 significantly inhibited the in vivo growth and migration of CRC cells. A cytokine antibody array was utilized to obtain a more comprehensive profile revealing the differentially expressed cytokines in ORFV infection. Cytokines, such as IL‑7, IL‑13, IL‑15, CD27, CD30, pentraxin 3, and B lymphocyte chemoattractant (BLC), were upregulated. Axl, CXCL16, ANG‑3, MMP10, IFN‑γ R1 and VEGF‑B were downregulated. The results indicated that ORFV played roles in the regulation of key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis, and the cell cycle. Finally, data was presented to validate that ORFV infection induces oncolytic activity by enhancing apoptosis in vivo and in vitro. In conclusion, ORFV could be an oncolytic virus for CRC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Chen D, Wang R, Long M, Li W, Xiao B, Deng H, Weng K, Gong D, Liu F, Luo S, Luo S, et al: Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncol Rep 45: 535-546, 2021.
APA
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H. ... Hao, W. (2021). Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncology Reports, 45, 535-546. https://doi.org/10.3892/or.2020.7885
MLA
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H., Weng, K., Gong, D., Liu, F., Luo, S., Hao, W."Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11". Oncology Reports 45.2 (2021): 535-546.
Chicago
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H., Weng, K., Gong, D., Liu, F., Luo, S., Hao, W."Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11". Oncology Reports 45, no. 2 (2021): 535-546. https://doi.org/10.3892/or.2020.7885
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Wang R, Long M, Li W, Xiao B, Deng H, Weng K, Gong D, Liu F, Luo S, Luo S, et al: Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncol Rep 45: 535-546, 2021.
APA
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H. ... Hao, W. (2021). Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11. Oncology Reports, 45, 535-546. https://doi.org/10.3892/or.2020.7885
MLA
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H., Weng, K., Gong, D., Liu, F., Luo, S., Hao, W."Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11". Oncology Reports 45.2 (2021): 535-546.
Chicago
Chen, D., Wang, R., Long, M., Li, W., Xiao, B., Deng, H., Weng, K., Gong, D., Liu, F., Luo, S., Hao, W."Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11". Oncology Reports 45, no. 2 (2021): 535-546. https://doi.org/10.3892/or.2020.7885
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