Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March-2021 Volume 45 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2021 Volume 45 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma

  • Authors:
    • Agnieszka Szczygieł
    • Natalia Anger‑Góra
    • Katarzyna Węgierek‑Ciura
    • Jagoda Mierzejewska
    • Joanna Rossowska
    • Tomasz M. Goszczyński
    • Marta Świtalska
    • Elżbieta Pajtasz‑Piasecka
  • View Affiliations / Copyright

    Affiliations: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53‑114 Wroclaw, Poland
    Copyright: © Szczygieł et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 945-962
    |
    Published online on: January 11, 2021
       https://doi.org/10.3892/or.2021.7930
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Chemotherapy with low‑molecular weight compounds, despite elimination of cancer cells, entails adverse effects. To overcome this disadvantage, innovative drug delivery systems are being developed, including conjugation of macromolecular carriers with therapeutics, e.g. a nanoconjugate of hydroxyethyl starch and methotrexate (HES‑MTX). The purpose of the present study was to determine whether HES‑MTX, applied as a chemotherapeutic, is able to modulate the immune response and support the antitumor response generated by dendritic cells (DCs) used subsequently as immunotherapeutic vaccines. Therefore, MTX or HES‑MTX was administered, as sole treatment or combined with DC‑based vaccines, to MC38 colon carcinoma tumor‑bearing mice. Alterations in antitumor immune response were evaluated by multiparameter flow cytometry analyses and functional assays. The results demonstrated that the nanoconjugate possesses greater immunomodulatory potential than MTX as reflected by changes in the landscape of immune cells infiltrating the tumor and increased cytotoxicity of splenic lymphocytes. In contrast to MTX, therapy with HES‑MTX as sole treatment or combined with DC‑based vaccines, contributed to significant tumor growth inhibition. However, only treatment with HES‑MTX and DC‑based vaccines activated the systemic specific antitumor response. In conclusion, due to its immunomodulatory properties, the HES‑MTX nanoconjugate could become a potent anticancer agent used in both chemo‑ and chemoimmunotherapeutic treatment schemes.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Nars MS and Kaneno R: Immunomodulatory effects of low dose chemotherapy and perspectives of its combination with immunotherapy. Int J Cancer. 132:2471–2478. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Golombek SK, May JN, Theek B, Appold L, Drude N, Kiessling F and Lammers T: Tumor targeting via EPR: Strategies to enhance patient responses. Adv Drug Deliv Rev. 130:17–38. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Roma-Rodrigues C, Mendes R, Baptista PV and Fernandes AR: Targeting tumor microenvironment for cancer therapy. Int J Mol Sci. 20:8402019. View Article : Google Scholar

4 

Hanahan D and Coussens LM: Accessories to the crime: Functions of cells recruited to the tumor microenvironment. Cancer Cell. 21:309–322. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Whiteside TL: The tumor microenvironment and its role in promoting tumor growth. Oncogene. 27:5904–5912. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Jahchan NS, Mujal AM, Pollack JL, Binnewies M, Sriram V, Reyno L and Krummel MF: Tuning the tumor myeloid microenvironment to fight cancer. Front Immunol. 10:16112019. View Article : Google Scholar : PubMed/NCBI

7 

Netea-Maier RT, Smit JWA and Netea MG: Metabolic changes in tumor cells and tumor-associated macrophages: A mutual relationship. Cancer Lett. 413:102–109. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Veglia F and Gabrilovich DI: Dendritic cells in cancer: The role revisited. Curr Opin Immunol. 45:43–51. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Szczygieł A and Pajtasz-Piasecka E: Between biology and medicine: Perspectives on the use of dendritic cells in anticancer therapy. Postepy Hig Med Dosw (Online). 71:921–941. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Constantino J, Gomes C, Falcão A, Cruz MT and Neves BM: Antitumor dendritic cell-based vaccines: Lessons from 20 years of clinical trials and future perspectives. Transl Res. 168:74–95. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Sabado RL, Balan S and Bhardwaj N: Dendritic cell-based immunotherapy. Cell Res. 27:74–95. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Palucka K and Banchereau J: Dendritic-cell-based therapeutic cancer vaccines. Immunity. 39:38–48. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Bol KF, Schreibelt G, Gerritsen WR, de Vries IJM and Figdor CG: Dendritic cell-based immunotherapy: State of the art and beyond. Clin Cancer Res. 22:1897–1906. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Goszczyński T, Nevozhay D, Wietrzyk J, Omar MS and Boratyński J: The antileukemic activity of modified fibrinogen-methotrexate conjugate. Biochim Biophys Acta. 1830:2526–2530. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Hao F, Lee RJ, Yang C, Zhong L, Sun Y, Dong S, Cheng Z, Teng L, Meng Q, Lu J, et al: Targeted Co-delivery of siRNA and methotrexate for tumor therapy via mixed micelles. Pharmaceutics. 11:922019. View Article : Google Scholar

16 

Chen Y, Zhang W, Huang Y, Gao F, Sha X, Lou K and Fang X: The therapeutic effect of methotrexate-conjugated Pluronic-based polymeric micelles on the folate receptor-rich tumors treatment. Int J Nanomedicine. 10:4043–4057. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Thomas TP, Huang B, Choi SK, Silpe JE, Kotlyar A, Desai AM, Zong H, Gam J, Joice M and Baker JR Jr: Polyvalent dendrimer-methotrexate as a folate receptor-targeted cancer therapeutic. Mol Pharm. 9:2669–2676. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Li MH, Choi SK, Thomas TP, Desai A, Lee KH, Kotlyar A, Banaszak Holl MM and Baker JR Jr: Dendrimer-based multivalent methotrexates as dual acting nanoconjugates for cancer cell targeting. Eur J Med Chem. 47:560–572. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Figueiró F, de Oliveira CP, Bergamin LS, Rockenbach L, Mendes FB, Jandrey EH, Moritz CE, Pettenuzzo LF, Sévigny J, Guterres SS, et al: Methotrexate up-regulates ecto-5′-nucleotidase/CD73 and reduces the frequency of T lymphocytes in the glioblastoma microenvironment. Purinergic Signal. 12:303–312. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Goszczyński TM, Filip-Psurska B, Kempińska K, Wietrzyk J and Boratyński J: Hydroxyethyl starch as an effective methotrexate carrier in anticancer therapy. Pharmacol Res Perspect. 2:e000472014. View Article : Google Scholar : PubMed/NCBI

21 

Goszczyński T, Boratyński J, Wietrzyk J, Filip-Psurska B and Kempińska K: Patent WO/2013/127885; A conjugate of methotrexate and Hydroxyethyl starch for use in the treatment cancer. 2013.

22 

Koźmiński P, Halik PK, Chesori R and Gniazdowska E: Overview of dual-acting drug methotrexate in different neurological diseases, autoimmune pathologies and cancers. Int J Mol Sci. 21:34832020. View Article : Google Scholar

23 

Abolmaali SS, Tamaddon AM and Dinarvand R: A review of therapeutic challenges and achievements of methotrexate delivery systems for treatment of cancer and rheumatoid arthritis. Cancer Chemother Pharmacol. 71:1115–1130. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Visentin M, Zhao R and Goldman ID: The antifolates. Hematol Oncol Clin North Am. 26629–648. (ix)2012. View Article : Google Scholar : PubMed/NCBI

25 

Brecher ME, Owen HG and Bandarenko N: Alternatives to albumin: Starch replacement for plasma exchange. J Clin Apher. 12:146–153. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Hoffmann S, Caysa H, Kuntsche J, Kreideweiss P, Leimert A, Mueller T and Mäder K: Carbohydrate plasma expanders for passive tumor targeting: In vitro and in vivo studies. Carbohydr Polym. 95:404–413. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Nogueira E, Sárria MP, Azoia NG, Antunes E, Loureiro A, Guimarães D, Noro J, Rollett A, Guebitz G and Cavaco-Paulo A: Internalization of methotrexate conjugates by folate receptor-α. Biochemistry. 57:6780–6786. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Thomas TP, Goonewardena SN, Majoros IJ, Kotlyar A, Cao Z, Leroueil PR and Baker JR: Folate-targeted nanoparticles show efficacy in the treatment of inflammatory arthritis. Arthritis Rheum. 63:2671–2680. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Wang S and Dormidontova EE: Nanoparticle targeting using multivalent ligands: Computer modeling. Soft Matter. 7:4435–4445. 2011. View Article : Google Scholar

30 

Fang J, Nakamura H and Maeda H: The EPR effect: Unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect. Adv Drug Deliv Rev. 63:136–151. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Maeda H: Tumor-selective delivery of macromolecular drugs via the EPR effect: Background and future prospects. Bioconjug Chem. 21:797–802. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Maeda H and Matsumura Y: EPR effect based drug design and clinical outlook for enhanced cancer chemotherapy. Adv Drug Deliv Rev. 63:129–130. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Shurin GV, Tourkova IL, Kaneno R and Shurin MR: Chemotherapeutic agents in noncytotoxic concentrations increase antigen presentation by dendritic cells via an IL-12-dependent mechanism. J Immunol. 183:137–144. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Kaneno R, Shurin GV, Tourkova IL and Shurin MR: Chemomodulation of human dendritic cell function by antineoplastic agents in low noncytotoxic concentrations. J Transl Med. 7:582009. View Article : Google Scholar : PubMed/NCBI

35 

Kaneno R, Shurin GV, Kaneno FM, Naiditch H, Luo J and Shurin MR: Chemotherapeutic agents in low noncytotoxic concentrations increase immunogenicity of human colon cancer cells. Cell Oncol (Dordr). 34:97–106. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Zhang L, Dermawan K, Jin M, Liu R, Zheng H, Xu L, Zhang Y, Cai Y, Chu Y and Xiong S: Differential impairment of regulatory T cells rather than effector T cells by paclitaxel-based chemotherapy. Clin Immunol. 129:219–229. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Sistigu A, Viaud S, Chaput N, Bracci L, Proietti E and Zitvogel L: Immunomodulatory effects of cyclophosphamide and implementations for vaccine design. Semin Immunopathol. 33:369–383. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Liu JY, Wu Y, Zhang XS, Yang JL, Li HL, Mao YQ, Wang Y, Cheng X, Li YQ, Xia JC, et al: Single administration of low dose cyclophosphamide augments the antitumor effect of dendritic cell vaccine. Cancer Immunol Immunother. 56:1597–1604. 2007. View Article : Google Scholar : PubMed/NCBI

39 

Rossowska J, Anger N, Kicielińska J, Pajtasz-Piasecka E, Bielawska-Pohl A, Wojas-Turek J and Duś D: Temporary elimination of IL-10 enhanced the effectiveness of cyclophosphamide and BMDC-based therapy by decrease of the suppressor activity of MDSCs and activation of antitumour immune response. Immunobiology. 220:389–398. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Rossowska J, Pajtasz-Piasecka E, Anger N, Wojas-Turek J, Kicielińska J, Piasecki E and Duś D: Cyclophosphamide and IL-12-transduced DCs enhance the antitumor activity of tumor antigen-stimulated DCs and reduce Tregs and MDSCs number. J Immunother. 37:427–439. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Pajtasz-Piasecka E, Szyda A, Rossowska J, Krawczenko A, Indrová M, Grabarczyk P, Wysocki P, Mackiewicz A and Duś D: Loss of tumorigenicity of murine colon carcinoma MC38/0 cell line after transduction with a retroviral vector carrying murine IL-12 genes. Folia Biol (Praha). 50:7–14. 2004.PubMed/NCBI

42 

Rossowska J, Pajtasz-Piasecka E, Szyda A, Krawczenko A, Zietara N and Dus D: Tumour antigen-loaded mouse dendritic cells maturing in the presence of inflammatory cytokines are potent activators of immune response in vitro but not in vivo. Oncol Rep. 21:1539–1549. 2009.PubMed/NCBI

43 

Thomas E, Jones G, de Souza P, Wardrop C and Wusteman F: Measuring blood volume with fluorescent-labeled hydroxyethyl starch. Crit Care Med. 28:627–631. 2000. View Article : Google Scholar : PubMed/NCBI

44 

Nevozhay D, Budzynska R, Kanska U, Jagiello M, Omar MS, Boratynski J and Opolski A: Antitumor properties and toxicity of dextran-methotrexate conjugates are dependent on the molecular weight of the carrier. Anticancer Res. 26:1135–1143. 2006.PubMed/NCBI

45 

Ciekot J, Goszczyński T and Boratyńskit J: Methods for methotrexate determination in macromolecular conjugates drug carrier. Acta Pol Pharm. 69:1342–1346. 2012.PubMed/NCBI

46 

Rossowska J, Anger N, Szczygieł A, Mierzejewska J and Pajtasz-Piasecka E: Reprogramming the murine colon cancer microenvironment using lentivectors encoding shRNA against IL-10 as a component of a potent DC-based chemoimmunotherapy. J Exp Clin Cancer Res. 37:1262018. View Article : Google Scholar : PubMed/NCBI

47 

Pajtasz-Piasecka E, Rossowska J, Szyda A, Krawczenko A and Dus D: Generation of anti-tumor response by JAWS II mouse dendritic cells transduced with murine interleukin 12 genes. Oncol Rep. 17:1249–1257. 2007.PubMed/NCBI

48 

Ji X, Guo H, Tang Q, Ma D and Xue W: A targeted nanocarrier based on polyspermine for the effective delivery of methotrexate in nasopharyngeal carcinoma. Mater Sci Eng C Mater Biol Appl. 81:48–56. 2017. View Article : Google Scholar : PubMed/NCBI

49 

Goos JACM, Cho A, Carter LM, Dilling TR, Davydova M, Mandleywala K, Puttick S, Gupta A, Price WS, Quinn JF, et al: Delivery of polymeric nanostars for molecular imaging and endoradiotherapy through the enhanced permeability and retention (EPR) effect. Theranostics. 10:567–584. 2020. View Article : Google Scholar : PubMed/NCBI

50 

Kobayashi H, Watanabe R and Choyke PL: Improving conventional enhanced permeability and retention (EPR) effects; what is the appropriate target? Theranostics. 4:81–89. 2013. View Article : Google Scholar : PubMed/NCBI

51 

Pajtasz-Piasecka E and Indrová M: Dendritic cell-based vaccines for the therapy of experimental tumors. Immunotherapy. 2:257–268. 2010. View Article : Google Scholar : PubMed/NCBI

52 

Zong J, Keskinov AA, Shurin GV and Shurin MR: Tumor-derived factors modulating dendritic cell function. Cancer Immunol Immunother. 65:821–833. 2016. View Article : Google Scholar : PubMed/NCBI

53 

Zhong H, Han B, Tourkova IL, Lokshin A, Rosenbloom A, Shurin MR and Shurin GV: Low-dose paclitaxel prior to intratumoral dendritic cell vaccine modulates intratumoral cytokine network and lung cancer growth. Clin Cancer Res. 13:5455–5462. 2007. View Article : Google Scholar : PubMed/NCBI

54 

Rossowska J, Anger N, Szczygieł A, Mierzejewska J and Pajtasz-Piasecka E: Intratumoral lentivector-mediated TGF-β1 gene downregulation as a potent strategy for enhancing the antitumor effect of therapy composed of cyclophosphamide and dendritic cells. Front Immunol. 8:7132017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Szczygieł A, Anger‑Góra N, Węgierek‑Ciura K, Mierzejewska J, Rossowska J, Goszczyński TM, Świtalska M and Pajtasz‑Piasecka E: Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma. Oncol Rep 45: 945-962, 2021.
APA
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T.M. ... Pajtasz‑Piasecka, E. (2021). Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma. Oncology Reports, 45, 945-962. https://doi.org/10.3892/or.2021.7930
MLA
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T. M., Świtalska, M., Pajtasz‑Piasecka, E."Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma". Oncology Reports 45.3 (2021): 945-962.
Chicago
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T. M., Świtalska, M., Pajtasz‑Piasecka, E."Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma". Oncology Reports 45, no. 3 (2021): 945-962. https://doi.org/10.3892/or.2021.7930
Copy and paste a formatted citation
x
Spandidos Publications style
Szczygieł A, Anger‑Góra N, Węgierek‑Ciura K, Mierzejewska J, Rossowska J, Goszczyński TM, Świtalska M and Pajtasz‑Piasecka E: Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma. Oncol Rep 45: 945-962, 2021.
APA
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T.M. ... Pajtasz‑Piasecka, E. (2021). Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma. Oncology Reports, 45, 945-962. https://doi.org/10.3892/or.2021.7930
MLA
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T. M., Świtalska, M., Pajtasz‑Piasecka, E."Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma". Oncology Reports 45.3 (2021): 945-962.
Chicago
Szczygieł, A., Anger‑Góra, N., Węgierek‑Ciura, K., Mierzejewska, J., Rossowska, J., Goszczyński, T. M., Świtalska, M., Pajtasz‑Piasecka, E."Immunomodulatory potential of anticancer therapy composed of methotrexate nanoconjugate and dendritic cell‑based vaccines in murine colon carcinoma". Oncology Reports 45, no. 3 (2021): 945-962. https://doi.org/10.3892/or.2021.7930
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team