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High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma

  • Authors:
    • Xiaoguang Li
    • Fengshuo Zhu
    • Zhonglong Liu
    • Xiao Tang
    • Yu Han
    • Junjian Jiang
    • Chunyue Ma
    • Yue He
  • View Affiliations / Copyright

    Affiliations: Department of Oral Maxillofacial‑Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Disease; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai 200011, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1182-1192
    |
    Published online on: January 18, 2021
       https://doi.org/10.3892/or.2021.7940
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Abstract

Dysregulation of Rab proteins has been observed in various types of cancer. Ectopic expression of Rab31, a member of the Rab protein family, is involved in cancer development and progression. However, the specific role and potential molecular mechanism underlying the functions of Rab31 remain largely unknown. Therefore, the current study aimed to investigate the functions of Rab31 in the development of cancer. Human oral squamous cell carcinoma (OSCC) samples were examined to determine the expression profile of Rab31 and its association with the clinicopathological characteristics of patients with OSCC. Knockdown of Rab31 expression with short hairpin RNA was performed to analyze the functions of Rab31 in vitro and in vivo. The expression of Rab31 was significantly elevated in human OSCC samples compared with that in normal oral mucosal epithelial tissues, and high expression levels were associated with high pathological grades. Furthermore, positive expression of Rab31 was associated with a poor prognosis in patients with OSCC. In addition, knockdown of Rab31 expression suppressed OSCC cell proliferation and induced apoptosis compared with those in the control‑transfected cells, which may have been caused by downregulated cyclin D1 and survivin expression and upregulated B‑cell lymphoma 2 expression. The invasive ability of OSCC cells was also abrogated by Rab31 silencing compared with that in the control‑transfected cells, which was associated with downregulated N‑cadherin and matrix metalloproteinase‑9 expression levels and upregulated levels of E‑cadherin expression. Furthermore, silencing Rab31 in OSCC cell lines, when compared with the control‑transfected cells, significantly reduced tumor growth and inhibited the expression of survivin, Ki‑67 and N‑cadherin in vivo. By contrast, the expression levels of E‑cadherin were increased. Taken together, the results of the present study supported important roles for Rab31 in regulating OSCC cell proliferation, apoptosis and invasion and may facilitate the identification of a new therapeutic target for the treatment of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Zhu F, Liu Z, Tang X, Han Y, Jiang J, Ma C and He Y: High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma. Oncol Rep 45: 1182-1192, 2021.
APA
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J. ... He, Y. (2021). High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma. Oncology Reports, 45, 1182-1192. https://doi.org/10.3892/or.2021.7940
MLA
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J., Ma, C., He, Y."High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma". Oncology Reports 45.3 (2021): 1182-1192.
Chicago
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J., Ma, C., He, Y."High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma". Oncology Reports 45, no. 3 (2021): 1182-1192. https://doi.org/10.3892/or.2021.7940
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Zhu F, Liu Z, Tang X, Han Y, Jiang J, Ma C and He Y: High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma. Oncol Rep 45: 1182-1192, 2021.
APA
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J. ... He, Y. (2021). High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma. Oncology Reports, 45, 1182-1192. https://doi.org/10.3892/or.2021.7940
MLA
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J., Ma, C., He, Y."High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma". Oncology Reports 45.3 (2021): 1182-1192.
Chicago
Li, X., Zhu, F., Liu, Z., Tang, X., Han, Y., Jiang, J., Ma, C., He, Y."High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma". Oncology Reports 45, no. 3 (2021): 1182-1192. https://doi.org/10.3892/or.2021.7940
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