LINC00665 functions as a competitive endogenous RNA to regulate AGTR1 expression by sponging miR‑34a‑5p in glioma

Dai,Yongyue; Zhang,Yucheng; Hao,Maolin; Zhu,Renwu

doi:10.3892/or.2021.7949

LINC00665 functions as a competitive endogenous RNA to regulate AGTR1 expression by sponging miR‑34a‑5p in glioma

Authors:
- Yongyue Dai
- Yucheng Zhang
- Maolin Hao
- Renwu Zhu
View Affiliations

Affiliations: Department of Pathophysiology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of General Surgery, Wenzhou Hospital Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang 325000, P.R. China
Published online on: January 22, 2021 https://doi.org/10.3892/or.2021.7949
Pages: 1202-1212
Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Glioma is the most aggressive tumor of the central nervous system. Long non‑coding RNAs (lncRNAs) may be involved in modulating tumor generation. The present study analyzed an lncRNA microarray of glioma and selected long intergenic non‑protein coding RNA 665 (LINC00665) as the research object. The mode of expression and biological function of LINC00665 in glioma were assessed using lncRNA microarray and RT‑qPCR analyses. Gain‑of‑function assays and/or loss‑of‑function assays were implemented to explore the role of LINC00665 in the progression of glioma. Dual‑luciferase reporter and RNA immunoprecipitation assays explored the downstream molecular mechanism of LINC00665. The function of the molecular pathway in progression of glioma was analyzed using rescue assays. High expression of LINC00665 was marked in glioma tissues and cells, which correlated with an unsatisfactory prognosis. Upregulation of LINC00665 significantly promoted the proliferation and invasion of glioma cells. LINC00665 acted as a competing endogenous RNA by sponging miR‑34a‑5p to upregulate angiotensin II receptor type 1 (AGTR1). LINC00665 promoted the progression of glioma by acting as a competitive endogenous RNA to competitively bind to miR‑34a‑5p and mediate AGTR1 expression.

View Figures

View References

1	Deng MY, Sill M, Sturm D, Stichel D, Witt H, Ecker J, Wittmann A, Schittenhelm J, Ebinger M, Schuhmann MU, et al: Diffuse glioneuronal tumour with oligodendroglioma-like features and nuclear clusters (DGONC)-a molecularly-defined glioneuronal CNS tumour class displaying recurrent monosomy 14. Neuropathol Appl Neurobiol. 46:422–430. 2019. View Article : Google Scholar
2	Xi J, Sun Q, Ma L and Kang J: Long non-coding RNAs in glioma progression. Cancer Lett. 419:203–209. 2018. View Article : Google Scholar : PubMed/NCBI
3	Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI
4	Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A and Bray F: Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int J Cancer. 144:1941–1953. 2019. View Article : Google Scholar : PubMed/NCBI
5	Saxena S and Jha S: Role of NOD-like receptors in glioma angiogenesis: Insights into future therapeutic interventions. Cytokine Growth Factor Rev. 34:15–26. 2017. View Article : Google Scholar : PubMed/NCBI
6	Rynkeviciene R, Simiene J, Strainiene E, Stankevicius V, Usinskiene J, Miseikyte Kaubriene E, Meskinyte I, Cicenas J and Suziedelis K: Non-coding RNAs in glioma. Cancers (Basel). 11:172018. View Article : Google Scholar
7	Wang Q, Li Q, Zhou P, Deng D, Xue L, Shao N, Peng Y and Zhi F: Upregulation of the long non-coding RNA SNHG1 predicts poor prognosis, promotes cell proliferation and invasion, and reduces apoptosis in glioma. Biomed Pharmacother. 91:906–911. 2017. View Article : Google Scholar : PubMed/NCBI
8	Gao Y, Yu H, Liu Y, Liu X, Zheng J, Ma J, Gong W, Chen J, Zhao L, Tian Y and Xue Y: Long non-coding RNA HOXA-AS2 regulates malignant glioma behaviors and vasculogenic mimicry formation via the MiR-373/EGFR axis. Cell Physiol Biochem. 45:131–147. 2018. View Article : Google Scholar : PubMed/NCBI
9	Lorenzen JM and Thum T: Long noncoding RNAs in kidney and cardiovascular diseases. Nat Rev Nephrol. 12:360–373. 2016. View Article : Google Scholar : PubMed/NCBI
10	Sun W, Yang Y, Xu C and Guo J: Regulatory mechanisms of long noncoding RNAs on gene expression in cancers. Cancer Genet. 216-217:105–110. 2017. View Article : Google Scholar : PubMed/NCBI
11	Dastmalchi N, Safaralizadeh R and Nargesi MM: LncRNAs: Potential novel prognostic and diagnostic biomarkers in colorectal cancer. Curr Med Chem. 27:5067–5077. 2020. View Article : Google Scholar : PubMed/NCBI
12	Lu Q, Gong W, Wang J, Ji K, Sun X, Xu C, Du L, Wang Y and Liu Q: Analysis of changes to lncRNAs and their target mRNAs in murine jejunum after radiation treatment. J Cell Mol Med. 22:6357–6367. 2018. View Article : Google Scholar : PubMed/NCBI
13	Sallam T, Jones M, Thomas BJ, Wu X, Gilliland T, Qian K, Eskin A, Casero D, Zhang Z, Sandhu J, et al: Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat Med. 24:304–312. 2018. View Article : Google Scholar : PubMed/NCBI
14	Li R and Fox AH: SPArking interest in the long noncoding RNA world: A new class of 5′SnoRNA-stabilized LncRNA that influences alternative splicing. Mol Cell. 64:435–437. 2016. View Article : Google Scholar : PubMed/NCBI
15	Xu ZM, Huang F and Huang WQ: Angiogenic lncRNAs: A potential therapeutic target for ischaemic heart disease. Life Sci. 211:157–171. 2018. View Article : Google Scholar : PubMed/NCBI
16	Tripathi MK, Doxtater K, Keramatnia F, Zacheaus C, Yallapu MM, Jaggi M and Chauhan SC: Role of lncRNAs in ovarian cancer: Defining new biomarkers for therapeutic purposes. Drug Discov Today. 23:1635–1643. 2018. View Article : Google Scholar : PubMed/NCBI
17	Chandra Gupta S and Nandan Tripathi Y: Potential of long non-coding RNAs in cancer patients: From biomarkers to therapeutic targets. Int J Cancer. 140:1955–1967. 2017. View Article : Google Scholar : PubMed/NCBI
18	Tamang S, Acharya V, Roy D, Sharma R, Aryaa A, Sharma U, Khandelwal A, Prakash H, Vasquez KM and Jain A: SNHG12: An LncRNA as a potential therapeutic target and biomarker for human cancer. Front Oncol. 9:9012019. View Article : Google Scholar : PubMed/NCBI
19	Chen J, Wang R, Zhang K and Chen LB: Long non-coding RNAs in non-small cell lung cancer as biomarkers and therapeutic targets. J Cell Mol Med. 18:2425–2436. 2014. View Article : Google Scholar : PubMed/NCBI
20	Yang B, Bai Q, Chen H, Su K and Gao C: LINC00665 induces gastric cancer progression through activating Wnt signaling pathway. J Cell Biochem. 121:2268–2276. 2020. View Article : Google Scholar : PubMed/NCBI
21	Qi H, Xiao Z and Wang Y: Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis. Onco Targets Ther. 12:6981–6990. 2019. View Article : Google Scholar : PubMed/NCBI
22	Liu X, Lu X, Zhen F, Jin S, Yu T, Zhu Q, Wang W, Xu K, Yao J and Guo R: LINC00665 induces acquired resistance to gefitinib through recruiting EZH2 and activating PI3K/AKT pathway in NSCLC. Mol Ther Nucleic Acids. 16:155–161. 2019. View Article : Google Scholar : PubMed/NCBI
23	Cong Z, Diao Y, Xu Y, Li X, Jiang Z, Shao C, Ji S, Shen Y, De W and Qiang Y: Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98. Cell Death Dis. 10:842019. View Article : Google Scholar : PubMed/NCBI
24	Shan Y and Li P: Long intergenic non-protein coding RNA 665 regulates viability, apoptosis, and autophagy via the MiR-186-5p/MAP4K3 axis in hepatocellular carcinoma. Yonsei Med J. 60:842–853. 2019. View Article : Google Scholar : PubMed/NCBI
25	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
26	Cui CL, Li YN, Cui XY and Wu X: lncRNA XIST promotes the progression of laryngeal squamous cell carcinoma by sponging miR-144 to regulate IRS1 expression. Oncol Rep. 43:525–535. 2020.PubMed/NCBI
27	Bao W, Cao F, Ni S, Yang J, Li H, Su Z and Zhao B: lncRNA FLVCR1-AS1 regulates cell proliferation, migration and invasion by sponging miR-485-5p in human cholangiocarcinoma. Oncol Lett. 18:2240–2247. 2019.PubMed/NCBI
28	Wang Y, Zeng X, Wang N, Zhao W, Zhang X, Teng S, Zhang Y and Lu Z: Long noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcoma. Mol Cancer. 17:892018. View Article : Google Scholar : PubMed/NCBI
29	Miao H, Wang L, Zhan H, Dai J, Chang Y, Wu F, Liu T, Liu Z, Gao C, Li L and Song X: A long noncoding RNA distributed in both nucleus and cytoplasm operates in the PYCARD-regulated apoptosis by coordinating the epigenetic and translational regulation. PLoS Genet. 15:e10081442019. View Article : Google Scholar : PubMed/NCBI
30	Xu H, Zhao G, Zhang Y, Jiang H, Wang W, Zhao D, Yu H and Qi L: Long non-coding RNA PAXIP1-AS1 facilitates cell invasion and angiogenesis of glioma by recruiting transcription factor ETS1 to upregulate KIF14 expression. J Exp Clin Cancer Res. 38:4862019. View Article : Google Scholar : PubMed/NCBI
31	Liu Q, Yu W, Zhu S, Cheng K, Xu H, Lv Y, Long X, Ma L, Huang J, Sun S and Wang K: Long noncoding RNA GAS5 regulates the proliferation, migration, and invasion of glioma cells by negatively regulating miR-18a-5p. J Cell Physiol. 234:757–768. 2018. View Article : Google Scholar : PubMed/NCBI
32	Li J, Guo W, Xue W, Xu P, Deng Z, Zhang D, Zheng S and Qiu X: Long noncoding RNA AURKAPS1 potentiates malignant hepatocellular carcinoma progression by regulating miR-142, miR-155 and miR-182. Sci Rep. 9:196452019. View Article : Google Scholar : PubMed/NCBI
33	Wang Y, Jiang F, Xiong Y, Cheng X, Qiu Z and Song R: LncRNA TTN-AS1 sponges miR-376a-3p to promote colorectal cancer progression via upregulating KLF15. Life Sci. 244:1169362020. View Article : Google Scholar : PubMed/NCBI
34	Gao J, Li N, Dong Y, Li S, Xu L, Li X, Li Y, Li Z, Ng SS, Sung JJ, et al: miR-34a-5p suppresses colorectal cancer metastasis and predicts recurrence in patients with stage II/III colorectal cancer. Oncogene. 34:4142–4152. 2015. View Article : Google Scholar : PubMed/NCBI
35	Ma S, Fu T, Zhao S and Gao M: MicroRNA-34a-5p suppresses tumorigenesis and progression of glioma and potentiates Temozolomide-induced cytotoxicity for glioma cells by targeting HMGA2. Eur J Pharmacol. 852:42–50. 2019. View Article : Google Scholar : PubMed/NCBI
36	Xu H, Zhang Y, Qi L, Ding L, Jiang H and Yu H: NFIX circular RNA promotes glioma progression by regulating miR-34a-5p via notch signaling pathway. Front Mol Neurosci. 11:2252018. View Article : Google Scholar : PubMed/NCBI
37	Di Bari M, Bevilacqua V, De Jaco A, Laneve P, Piovesana R, Trobiani L, Talora C, Caffarelli E and Tata AM: Mir-34a-5p mediates cross-talk between M2 muscarinic receptors and notch-1/EGFR pathways in U87MG glioblastoma cells: Implication in cell proliferation. Int J Mol Sci. 19:16312018. View Article : Google Scholar
38	Ma Y, Xia Z, Ye C, Lu C, Zhou S, Pan J, Liu C, Zhang J, Liu T, Hu T, et al: AGTR1 promotes lymph node metastasis in breast cancer by upregulating CXCR4/SDF-1α and inducing cell migration and invasion. Aging (Albany NY). 11:3969–3992. 2019. View Article : Google Scholar : PubMed/NCBI