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Article

Lipid droplet storage promotes murine pancreatic tumor growth

  • Authors:
    • Jeremy J. Grachan
    • Martin Kery
    • Amato J. Giaccia
    • Nicholas C. Denko
    • Ioanna Papandreou
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA, Department of Radiation Oncology, Stanford University, Stanford, CA 94305, USA
  • Article Number: 21
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    Published online on: February 9, 2021
       https://doi.org/10.3892/or.2021.7972
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Abstract

Hypoxia Inducible Lipid Droplet Associated (HILPDA) is frequently overexpressed in tumors and promotes neutral lipid storage. The impact of Hilpda on pancreatic ductal adenocarcinoma (PDAC) tumor growth is not known. In order to evaluate Hilpda‑dependent lipid storage mechanisms, expression of Hilpda in murine pancreatic cells (KPC) was genetically manipulated. Lipid droplet (LD) abundance and triglyceride content in vitro were measured, and model tumor growth in nu/nu mice was determined. The results showed that excess lipid supply increased triglyceride storage and LD formation in KPC cells in a HILPDA‑dependent manner. Contrary to published results, inhibition of Adipose Triglyceride Lipase (ATGL) did not ameliorate the triglyceride abundance differences between Hilpda WT and KO cells. Hilpda ablation significantly decreased the growth rate of model tumors in immunocompromised mice. In conclusion, Hilpda is a positive regulator of triglyceride storage and lipid droplet formation in murine pancreatic cancer cells in vitro and lipid accumulation and tumor growth in vivo. Our data suggest that deregulated ATGL is not responsible for the absence of LDs in KO cells in this context.
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Copy and paste a formatted citation
Spandidos Publications style
Grachan JJ, Kery M, Giaccia AJ, Denko NC and Papandreou I: Lipid droplet storage promotes murine pancreatic tumor growth. Oncol Rep 45: 21, 2021.
APA
Grachan, J.J., Kery, M., Giaccia, A.J., Denko, N.C., & Papandreou, I. (2021). Lipid droplet storage promotes murine pancreatic tumor growth. Oncology Reports, 45, 21. https://doi.org/10.3892/or.2021.7972
MLA
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45.4 (2021): 21.
Chicago
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45, no. 4 (2021): 21. https://doi.org/10.3892/or.2021.7972
Copy and paste a formatted citation
x
Spandidos Publications style
Grachan JJ, Kery M, Giaccia AJ, Denko NC and Papandreou I: Lipid droplet storage promotes murine pancreatic tumor growth. Oncol Rep 45: 21, 2021.
APA
Grachan, J.J., Kery, M., Giaccia, A.J., Denko, N.C., & Papandreou, I. (2021). Lipid droplet storage promotes murine pancreatic tumor growth. Oncology Reports, 45, 21. https://doi.org/10.3892/or.2021.7972
MLA
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45.4 (2021): 21.
Chicago
Grachan, J. J., Kery, M., Giaccia, A. J., Denko, N. C., Papandreou, I."Lipid droplet storage promotes murine pancreatic tumor growth". Oncology Reports 45, no. 4 (2021): 21. https://doi.org/10.3892/or.2021.7972
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